Oxidative Stress and DNA Damage in Human Gastric Carcinoma: 8-Oxo-7 ' 8-dihydro-2 '-deoxyguanosine (8-oxo-dG) as a Possible Tumor Marker

We characterized the oxidative stress (OS) status by the levels of reduced/oxidized glutathione (GSH/GSSG), malondialdehyde (MDA) and the mutagenic base 8-oxo-7'8-dihydro-2'-deoxyguanosine (8-oxo-dG) in human gastric carcinoma (HGC) samples and compared the results with normal tissue from...

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Autores: Borrego, S, Vazquez, A, Dasi, F, Cerda, C, Iradi, A, Tormos, C, Sanchez, JM, Bagan, L, Boix, J, Zaragoza, C, Camps, J, Saez, G
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Institución:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
Repositorio:r-FISABIO. Repositorio Institucional de Producción Científica
OAI Identifier:oai:fisabio.fundanetsuite.com:p10671
Acceso en línea:https://fisabio.portalinvestigacion.com/publicaciones/10671
Access Level:acceso abierto
Palabra clave:8-oxo-dG
DNA repair enzymes
gastric cancer
oxidative stress
tumor marker
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spelling Oxidative Stress and DNA Damage in Human Gastric Carcinoma: 8-Oxo-7 ' 8-dihydro-2 '-deoxyguanosine (8-oxo-dG) as a Possible Tumor MarkerBorrego, SVazquez, ADasi, FCerda, CIradi, ATormos, CSanchez, JMBagan, LBoix, JZaragoza, CCamps, JSaez, G8-oxo-dGDNA repair enzymesgastric canceroxidative stresstumor markerWe characterized the oxidative stress (OS) status by the levels of reduced/oxidized glutathione (GSH/GSSG), malondialdehyde (MDA) and the mutagenic base 8-oxo-7'8-dihydro-2'-deoxyguanosine (8-oxo-dG) in human gastric carcinoma (HGC) samples and compared the results with normal tissue from the same patients. We also analyzed 8-oxo-dG in peripheral mononuclear cells (PMNC) and urine from healthy control subjects and in affected patients in the basal state and one, three, six, nine and twelve months after tumor resection. The levels of DNA repair enzyme mRNA expression (hOGG1, RAD51, MUYTH and MTH1) were determined in tumor specimens and compared with normal mucosa. Tumor specimens exhibited increased levels of MDA and 8-oxo-dG compared with normal gastric tissue. GSH levels were also increased, while GSSG levels remained stable. DNA repair enzyme mRNA expression was induced in the tumor tissues. Levels of 8-oxo-dG were significantly elevated in both urine and PMNC of gastric cancer patients compared with healthy controls. After gastrectomy, the levels of the damaged base in urine and PMNC decreased progressively to values close to those found in the healthy population. The high levels of 8-oxo-dG in urine may be related to the increased induction of DNA repair activity in tumor tissue, and the changes observed after tumor resection support its potential use as a tumor marker.MDPI2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fisabio.portalinvestigacion.com/publicaciones/10671INTERNATIONAL JOURNAL OF MOLECULAR SCIENCESISSN: 16616596ISSNe: 14220067reponame:r-FISABIO. Repositorio Institucional de Producción Científicainstname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)Inglésinfo:eu-repo/semantics/openAccessoai:fisabio.fundanetsuite.com:p106712026-06-11T12:45:17Z
dc.title.none.fl_str_mv Oxidative Stress and DNA Damage in Human Gastric Carcinoma: 8-Oxo-7 ' 8-dihydro-2 '-deoxyguanosine (8-oxo-dG) as a Possible Tumor Marker
title Oxidative Stress and DNA Damage in Human Gastric Carcinoma: 8-Oxo-7 ' 8-dihydro-2 '-deoxyguanosine (8-oxo-dG) as a Possible Tumor Marker
spellingShingle Oxidative Stress and DNA Damage in Human Gastric Carcinoma: 8-Oxo-7 ' 8-dihydro-2 '-deoxyguanosine (8-oxo-dG) as a Possible Tumor Marker
Borrego, S
8-oxo-dG
DNA repair enzymes
gastric cancer
oxidative stress
tumor marker
title_short Oxidative Stress and DNA Damage in Human Gastric Carcinoma: 8-Oxo-7 ' 8-dihydro-2 '-deoxyguanosine (8-oxo-dG) as a Possible Tumor Marker
title_full Oxidative Stress and DNA Damage in Human Gastric Carcinoma: 8-Oxo-7 ' 8-dihydro-2 '-deoxyguanosine (8-oxo-dG) as a Possible Tumor Marker
title_fullStr Oxidative Stress and DNA Damage in Human Gastric Carcinoma: 8-Oxo-7 ' 8-dihydro-2 '-deoxyguanosine (8-oxo-dG) as a Possible Tumor Marker
title_full_unstemmed Oxidative Stress and DNA Damage in Human Gastric Carcinoma: 8-Oxo-7 ' 8-dihydro-2 '-deoxyguanosine (8-oxo-dG) as a Possible Tumor Marker
title_sort Oxidative Stress and DNA Damage in Human Gastric Carcinoma: 8-Oxo-7 ' 8-dihydro-2 '-deoxyguanosine (8-oxo-dG) as a Possible Tumor Marker
dc.creator.none.fl_str_mv Borrego, S
Vazquez, A
Dasi, F
Cerda, C
Iradi, A
Tormos, C
Sanchez, JM
Bagan, L
Boix, J
Zaragoza, C
Camps, J
Saez, G
author Borrego, S
author_facet Borrego, S
Vazquez, A
Dasi, F
Cerda, C
Iradi, A
Tormos, C
Sanchez, JM
Bagan, L
Boix, J
Zaragoza, C
Camps, J
Saez, G
author_role author
author2 Vazquez, A
Dasi, F
Cerda, C
Iradi, A
Tormos, C
Sanchez, JM
Bagan, L
Boix, J
Zaragoza, C
Camps, J
Saez, G
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv 8-oxo-dG
DNA repair enzymes
gastric cancer
oxidative stress
tumor marker
topic 8-oxo-dG
DNA repair enzymes
gastric cancer
oxidative stress
tumor marker
description We characterized the oxidative stress (OS) status by the levels of reduced/oxidized glutathione (GSH/GSSG), malondialdehyde (MDA) and the mutagenic base 8-oxo-7'8-dihydro-2'-deoxyguanosine (8-oxo-dG) in human gastric carcinoma (HGC) samples and compared the results with normal tissue from the same patients. We also analyzed 8-oxo-dG in peripheral mononuclear cells (PMNC) and urine from healthy control subjects and in affected patients in the basal state and one, three, six, nine and twelve months after tumor resection. The levels of DNA repair enzyme mRNA expression (hOGG1, RAD51, MUYTH and MTH1) were determined in tumor specimens and compared with normal mucosa. Tumor specimens exhibited increased levels of MDA and 8-oxo-dG compared with normal gastric tissue. GSH levels were also increased, while GSSG levels remained stable. DNA repair enzyme mRNA expression was induced in the tumor tissues. Levels of 8-oxo-dG were significantly elevated in both urine and PMNC of gastric cancer patients compared with healthy controls. After gastrectomy, the levels of the damaged base in urine and PMNC decreased progressively to values close to those found in the healthy population. The high levels of 8-oxo-dG in urine may be related to the increased induction of DNA repair activity in tumor tissue, and the changes observed after tumor resection support its potential use as a tumor marker.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fisabio.portalinvestigacion.com/publicaciones/10671
url https://fisabio.portalinvestigacion.com/publicaciones/10671
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN: 16616596
ISSNe: 14220067
reponame:r-FISABIO. Repositorio Institucional de Producción Científica
instname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
instname_str Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
reponame_str r-FISABIO. Repositorio Institucional de Producción Científica
collection r-FISABIO. Repositorio Institucional de Producción Científica
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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