Nucleosome dynamics and analysis in breast cancer cells
Genome-wide analysis of the nucleosome positioning and histone H1 isoform content of the T47D breast cancer cell line has found a number of observations, namely that with a gentle digestion of microccocal nuclease (MNase), a nucleosome is visible just upstream of the transcription start site, in the...
| Author: | |
|---|---|
| Format: | doctoral thesis |
| Status: | Published version |
| Publication Date: | 2014 |
| Country: | España |
| Institution: | CBUC, CESCA |
| Repository: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/328416 |
| Online Access: | http://hdl.handle.net/10803/328416 |
| Access Level: | Open access |
| Keyword: | Epigenètica Histona Nucleosoma Programari Genoma ADN Càncer de pit Progesterona Bioinformàtica Genòmica Hormona Regulació genètica Transcripcio Genomics Histone Epigenetics DNA Breast cancer Progesterone 575 |
| Summary: | Genome-wide analysis of the nucleosome positioning and histone H1 isoform content of the T47D breast cancer cell line has found a number of observations, namely that with a gentle digestion of microccocal nuclease (MNase), a nucleosome is visible just upstream of the transcription start site, in the region known as the “nucleosome-free region” (NFR). H1 isoforms bind to chromatin mainly in a redundant manner, but H1.2 and H1.3 show some specificity while H1.5 increases its binding dramatically after a progesterone stimulus. In the course of these studies, a general-purpose software package was developed for the manipulation and analysis of bigWig files, a data format for storing continuous signal data assigned to genome coordinates |
|---|