In vitro permeability of saponins and sapogenins from seed extracts by the parallel artificial membrane permeability assay: Effect of in vitro gastrointestinal digestion

The permeability of saponins and sapogenins from fenugreek and quinoa extracts, as well as dioscin and diosgenin, was evaluated by the parallel artificial membrane permeability assay (PAMPA). The effect of the digestion process on permeability was determined, with previous development of a gastroint...

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Detalles Bibliográficos
Autores: Navarro del Hierro, Joaquín, Piazzini, Vieri, Reglero, Guillermo, Martín, Diana, Bergonzi, Maria Camilla
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/220147
Acceso en línea:http://hdl.handle.net/10261/220147
Access Level:acceso abierto
Palabra clave:PAMPA
Gastrointestinal digestion
Saponins
Fenugreek
Quinoa
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spelling In vitro permeability of saponins and sapogenins from seed extracts by the parallel artificial membrane permeability assay: Effect of in vitro gastrointestinal digestionNavarro del Hierro, JoaquínPiazzini, VieriReglero, GuillermoMartín, DianaBergonzi, Maria CamillaPAMPAGastrointestinal digestionSaponinsFenugreekQuinoaThe permeability of saponins and sapogenins from fenugreek and quinoa extracts, as well as dioscin and diosgenin, was evaluated by the parallel artificial membrane permeability assay (PAMPA). The effect of the digestion process on permeability was determined, with previous development of a gastrointestinal process coupled to PAMPA. Saponins from both seeds displayed a moderate-to-poor permeability (>1 × 10–6 cm/s), although the digestion enhanced their permeability values in the order of 10–5 cm/s (p < 0.001). Sapogenins exhibited a similar permeability to that of saponins, although the digestion enhanced the permeability of sapogenins from quinoa (1.14 ± 0.47 × 10–5 cm/s) but not from fenugreek (2.33 ± 0.99 × 10–6 cm/s). An overall positive impact of coexisting lipids on the permeability was evidenced. PAMPA is shown as a useful, rapid, and easy tool for assessing the permeability of bioactive compounds from complex matrices, with the previous gastrointestinal process being a relevant step.This work was supported by the Ministerio de Economía y Competitividad, Spain (AGL2016-76736-C3-1-R) and the Community of Madrid, Spain (ALIBIRD2020-CM, S2018/BAA-4343). J.N.d.H. thanks the Ministerio de Educacion, Cultura y Deporte for funding his research with a FPU predoctoral contract (FPU 15/04236).Peer reviewedAmerican Chemical SocietyMinisterio de Economía y Competitividad (España)Comunidad de MadridMinisterio de Educación, Cultura y Deporte (España)Martín, Diana [0000-0002-1082-9280]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/220147reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-76736-C3-1-RS2018/BAA-4343/ALIBIRD2020-CMhttps://doi.org/10.1021/acs.jafc.9b07182Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2201472026-05-22T06:33:51Z
dc.title.none.fl_str_mv In vitro permeability of saponins and sapogenins from seed extracts by the parallel artificial membrane permeability assay: Effect of in vitro gastrointestinal digestion
title In vitro permeability of saponins and sapogenins from seed extracts by the parallel artificial membrane permeability assay: Effect of in vitro gastrointestinal digestion
spellingShingle In vitro permeability of saponins and sapogenins from seed extracts by the parallel artificial membrane permeability assay: Effect of in vitro gastrointestinal digestion
Navarro del Hierro, Joaquín
PAMPA
Gastrointestinal digestion
Saponins
Fenugreek
Quinoa
title_short In vitro permeability of saponins and sapogenins from seed extracts by the parallel artificial membrane permeability assay: Effect of in vitro gastrointestinal digestion
title_full In vitro permeability of saponins and sapogenins from seed extracts by the parallel artificial membrane permeability assay: Effect of in vitro gastrointestinal digestion
title_fullStr In vitro permeability of saponins and sapogenins from seed extracts by the parallel artificial membrane permeability assay: Effect of in vitro gastrointestinal digestion
title_full_unstemmed In vitro permeability of saponins and sapogenins from seed extracts by the parallel artificial membrane permeability assay: Effect of in vitro gastrointestinal digestion
title_sort In vitro permeability of saponins and sapogenins from seed extracts by the parallel artificial membrane permeability assay: Effect of in vitro gastrointestinal digestion
dc.creator.none.fl_str_mv Navarro del Hierro, Joaquín
Piazzini, Vieri
Reglero, Guillermo
Martín, Diana
Bergonzi, Maria Camilla
author Navarro del Hierro, Joaquín
author_facet Navarro del Hierro, Joaquín
Piazzini, Vieri
Reglero, Guillermo
Martín, Diana
Bergonzi, Maria Camilla
author_role author
author2 Piazzini, Vieri
Reglero, Guillermo
Martín, Diana
Bergonzi, Maria Camilla
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Comunidad de Madrid
Ministerio de Educación, Cultura y Deporte (España)
Martín, Diana [0000-0002-1082-9280]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv PAMPA
Gastrointestinal digestion
Saponins
Fenugreek
Quinoa
topic PAMPA
Gastrointestinal digestion
Saponins
Fenugreek
Quinoa
description The permeability of saponins and sapogenins from fenugreek and quinoa extracts, as well as dioscin and diosgenin, was evaluated by the parallel artificial membrane permeability assay (PAMPA). The effect of the digestion process on permeability was determined, with previous development of a gastrointestinal process coupled to PAMPA. Saponins from both seeds displayed a moderate-to-poor permeability (>1 × 10–6 cm/s), although the digestion enhanced their permeability values in the order of 10–5 cm/s (p < 0.001). Sapogenins exhibited a similar permeability to that of saponins, although the digestion enhanced the permeability of sapogenins from quinoa (1.14 ± 0.47 × 10–5 cm/s) but not from fenugreek (2.33 ± 0.99 × 10–6 cm/s). An overall positive impact of coexisting lipids on the permeability was evidenced. PAMPA is shown as a useful, rapid, and easy tool for assessing the permeability of bioactive compounds from complex matrices, with the previous gastrointestinal process being a relevant step.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/220147
url http://hdl.handle.net/10261/220147
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-76736-C3-1-R
S2018/BAA-4343/ALIBIRD2020-CM
https://doi.org/10.1021/acs.jafc.9b07182
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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