DataSheet_1_Extracellular vesicles from Listeria monocytogenes-infected dendritic cells alert the innate immune response.pdf [Dataset]

Supplementary Figure 1. Isolated EVs present typical size and topology. Supplementary Figure 2. Protein profiling from total cell lysates and their derived EVs from WT and KO-HDAC6 BMDCs. Supplementary Figure 3. Enrichment in acetylated and ubiquitinated DC proteins upon Lm infection. Supplementary...

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Autores: Izquierdo-Serrano, Raúl, Fernández-Delgado, Irene, Moreno-Gonzalo, Olga, Martín-Gayo, Enrique, Calzada-Fraile, Diego, Ramírez-Huesca, Marta, Jorge, Inmaculada, Camafeita, Emilio, Abián, Joaquín, Vicente-Manzanares, Miguel, Veiga, Esteban, Vázquez, Jesús, Sánchez-Madrid, Francisco
Tipo de recurso: conjunto de datos
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/311748
Acceso en línea:http://hdl.handle.net/10261/311748
Access Level:acceso abierto
Palabra clave:Listeria monocytogenes infection
Dendritic cells
Extracellular vesicles
Immune alert
HDAC6
id ES_4e67f0e01105fc8024ddb69f082f73b6
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network_acronym_str ES
network_name_str España
repository_id_str
spelling DataSheet_1_Extracellular vesicles from Listeria monocytogenes-infected dendritic cells alert the innate immune response.pdf [Dataset]Izquierdo-Serrano, RaúlFernández-Delgado, IreneMoreno-Gonzalo, OlgaMartín-Gayo, EnriqueCalzada-Fraile, DiegoRamírez-Huesca, MartaJorge, InmaculadaCamafeita, EmilioAbián, JoaquínVicente-Manzanares, MiguelVeiga, EstebanVázquez, JesúsSánchez-Madrid, FranciscoListeria monocytogenes infectionDendritic cellsExtracellular vesiclesImmune alertHDAC6Supplementary Figure 1. Isolated EVs present typical size and topology. Supplementary Figure 2. Protein profiling from total cell lysates and their derived EVs from WT and KO-HDAC6 BMDCs. Supplementary Figure 3. Enrichment in acetylated and ubiquitinated DC proteins upon Lm infection. Supplementary Figure 4. Ubiquitination in K-48 and K-63 state in T lymphoblast total cell lysates and their derived EVs. Supplementary Figure 5. Pore filtration methods restrain Lm and do not induce strong antipathogenic responses. Supplementary Figure 6. IFN-β is detected following Lm infection. Table S1. List of antibodies used for Western-blot and Flow Cytometry and the used dilution. Table S2. List of primers, with their corresponding sequence, used for qPCR. Table S3: Protein quantification in total cell lysates Table S4: IPA analysis of total cell lysates: canonical pathways and diseases and functions category Table S5: Protein quantification in EVs Table S6: IPA analysis of EVs: diseases and functions category Table S7: Ubiquitinated and acetylated peptides in total cell lysates and EVs Table S8: Enrichment analysis of ubiquitinated and acetylated proteinsPeer reviewedFigshareConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/311748reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésIzquierdo-Serrano, Raúl; Fernández-Delgado, Irene; Moreno-Gonzalo, Olga; Martín-Gayo, Enrique; Calzada-Fraile, Diego; Ramírez-Huesca, Marta; Jorge, Inmaculada; Camafeita, Emilio; Abián, Joaquín; Vicente-Manzanares, Miguel; Veiga, Esteban; Vázquez, Jesús; Sánchez-Madrid, Francisco. DataSheet_1_Extracellular vesicles from Listeria monocytogenes-infected dendritic cells alert the innate immune response.pdf [Dataset]. http://dx.doi.org/10.3389/fimmu.2022.946358. http://hdl.handle.net/10261/288044https://doi.org/10.3389/fimmu.2022.946358.s001Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3117482026-05-22T06:33:51Z
dc.title.none.fl_str_mv DataSheet_1_Extracellular vesicles from Listeria monocytogenes-infected dendritic cells alert the innate immune response.pdf [Dataset]
title DataSheet_1_Extracellular vesicles from Listeria monocytogenes-infected dendritic cells alert the innate immune response.pdf [Dataset]
spellingShingle DataSheet_1_Extracellular vesicles from Listeria monocytogenes-infected dendritic cells alert the innate immune response.pdf [Dataset]
Izquierdo-Serrano, Raúl
Listeria monocytogenes infection
Dendritic cells
Extracellular vesicles
Immune alert
HDAC6
title_short DataSheet_1_Extracellular vesicles from Listeria monocytogenes-infected dendritic cells alert the innate immune response.pdf [Dataset]
title_full DataSheet_1_Extracellular vesicles from Listeria monocytogenes-infected dendritic cells alert the innate immune response.pdf [Dataset]
title_fullStr DataSheet_1_Extracellular vesicles from Listeria monocytogenes-infected dendritic cells alert the innate immune response.pdf [Dataset]
title_full_unstemmed DataSheet_1_Extracellular vesicles from Listeria monocytogenes-infected dendritic cells alert the innate immune response.pdf [Dataset]
title_sort DataSheet_1_Extracellular vesicles from Listeria monocytogenes-infected dendritic cells alert the innate immune response.pdf [Dataset]
dc.creator.none.fl_str_mv Izquierdo-Serrano, Raúl
Fernández-Delgado, Irene
Moreno-Gonzalo, Olga
Martín-Gayo, Enrique
Calzada-Fraile, Diego
Ramírez-Huesca, Marta
Jorge, Inmaculada
Camafeita, Emilio
Abián, Joaquín
Vicente-Manzanares, Miguel
Veiga, Esteban
Vázquez, Jesús
Sánchez-Madrid, Francisco
author Izquierdo-Serrano, Raúl
author_facet Izquierdo-Serrano, Raúl
Fernández-Delgado, Irene
Moreno-Gonzalo, Olga
Martín-Gayo, Enrique
Calzada-Fraile, Diego
Ramírez-Huesca, Marta
Jorge, Inmaculada
Camafeita, Emilio
Abián, Joaquín
Vicente-Manzanares, Miguel
Veiga, Esteban
Vázquez, Jesús
Sánchez-Madrid, Francisco
author_role author
author2 Fernández-Delgado, Irene
Moreno-Gonzalo, Olga
Martín-Gayo, Enrique
Calzada-Fraile, Diego
Ramírez-Huesca, Marta
Jorge, Inmaculada
Camafeita, Emilio
Abián, Joaquín
Vicente-Manzanares, Miguel
Veiga, Esteban
Vázquez, Jesús
Sánchez-Madrid, Francisco
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Listeria monocytogenes infection
Dendritic cells
Extracellular vesicles
Immune alert
HDAC6
topic Listeria monocytogenes infection
Dendritic cells
Extracellular vesicles
Immune alert
HDAC6
description Supplementary Figure 1. Isolated EVs present typical size and topology. Supplementary Figure 2. Protein profiling from total cell lysates and their derived EVs from WT and KO-HDAC6 BMDCs. Supplementary Figure 3. Enrichment in acetylated and ubiquitinated DC proteins upon Lm infection. Supplementary Figure 4. Ubiquitination in K-48 and K-63 state in T lymphoblast total cell lysates and their derived EVs. Supplementary Figure 5. Pore filtration methods restrain Lm and do not induce strong antipathogenic responses. Supplementary Figure 6. IFN-β is detected following Lm infection. Table S1. List of antibodies used for Western-blot and Flow Cytometry and the used dilution. Table S2. List of primers, with their corresponding sequence, used for qPCR. Table S3: Protein quantification in total cell lysates Table S4: IPA analysis of total cell lysates: canonical pathways and diseases and functions category Table S5: Protein quantification in EVs Table S6: IPA analysis of EVs: diseases and functions category Table S7: Ubiquitinated and acetylated peptides in total cell lysates and EVs Table S8: Enrichment analysis of ubiquitinated and acetylated proteins
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
http://purl.org/coar/resource_type/c_ddb1
Publisher's version
info:eu-repo/semantics/publishedVersion
format dataset
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/311748
url http://hdl.handle.net/10261/311748
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Izquierdo-Serrano, Raúl; Fernández-Delgado, Irene; Moreno-Gonzalo, Olga; Martín-Gayo, Enrique; Calzada-Fraile, Diego; Ramírez-Huesca, Marta; Jorge, Inmaculada; Camafeita, Emilio; Abián, Joaquín; Vicente-Manzanares, Miguel; Veiga, Esteban; Vázquez, Jesús; Sánchez-Madrid, Francisco. DataSheet_1_Extracellular vesicles from Listeria monocytogenes-infected dendritic cells alert the innate immune response.pdf [Dataset]. http://dx.doi.org/10.3389/fimmu.2022.946358. http://hdl.handle.net/10261/288044
https://doi.org/10.3389/fimmu.2022.946358.s001
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Figshare
publisher.none.fl_str_mv Figshare
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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