PEGylated and poloxamer-modified chitosan nanoparticles incorporating a lysine-based surfactant for pH-triggered doxorubicin release

The growing demand for efficient chemotherapy in many cancers requires novel approaches in target-delivery technologies. Nanomaterials with pH-responsive behavior appear to have potential ability to selectively release the encapsulated molecules by sensing the acidic tumor microenvironment or the lo...

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Autores: Scheeren, Laís E., Nogueira, Daniele R., Macedo, Letícia B., Vinardell Martínez-Hidalgo, Ma. Pilar, Mitjans Arnal, Montserrat, Infante Martínez-Pardo, Ma. Rosa, Rolim, Clarice M. B.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/96622
Acceso en línea:https://hdl.handle.net/2445/96622
Access Level:acceso abierto
Palabra clave:Medicaments antineoplàstics
Quitosan
Nanopartícules
Agents tensioactius
Sistemes d'alliberament de medicaments
Antineoplastic agents
Chitosan
Nanoparticles
Surface active agents
Drug delivery systems
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spelling PEGylated and poloxamer-modified chitosan nanoparticles incorporating a lysine-based surfactant for pH-triggered doxorubicin releaseScheeren, Laís E.Nogueira, Daniele R.Macedo, Letícia B.Vinardell Martínez-Hidalgo, Ma. PilarMitjans Arnal, MontserratInfante Martínez-Pardo, Ma. RosaRolim, Clarice M. B.Medicaments antineoplàsticsQuitosanNanopartículesAgents tensioactiusSistemes d'alliberament de medicamentsAntineoplastic agentsChitosanNanoparticlesSurface active agentsDrug delivery systemsThe growing demand for efficient chemotherapy in many cancers requires novel approaches in target-delivery technologies. Nanomaterials with pH-responsive behavior appear to have potential ability to selectively release the encapsulated molecules by sensing the acidic tumor microenvironment or the low pH found in endosomes. Likewise, polyethylene glycol (PEG)- and poloxamer-modified nanocarriers have been gaining attention regarding their potential to improve the effectiveness of cancer therapy. In this context, DOX-loaded pH-responsive nanoparticles (NPs) modified with PEG or poloxamer were prepared and the effects of these modifiers were evaluated on the overall characteristics of these nanostructures. Chitosan and tripolyphosphate were selected to form NPs by the interaction of oppositely charged compounds. A pH-sensitive lysine-based amphiphile (77KS) was used as a bioactive adjuvant. The strong dependence of 77KS ionization with pH makes this compound an interesting candidate to be used for the design of pH-sensitive devices. The physicochemical characterization of all NPs has been performed, and it was shown that the presence of 77KS clearly promotes a pH-triggered DOX release. Accelerated and continuous release patterns of DOX from CS-NPs under acidic conditions were observed regardless of the presence of PEG or poloxamer. Moreover, photodegradation studies have indicated that the lyophilization of NPs improved DOX stability under UVA radiation. Finally, cytotoxicity experiments have shown the ability of DOX-loaded CS-NPs to kill HeLa tumor cells. Hence, the overall results suggest that these pH-responsive CS-NPs are highly potent delivery systems to target tumor and intracellular environments, rendering them promising DOX carrier systems for cancer therapy.Elsevier B.V.2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/96622Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: http://dx.doi.org/10.1016/j.colsurfb.2015.11.049Colloids and Surfaces B-Biointerfaces, 2015, vol. 138, p. 117-127http://dx.doi.org/10.1016/j.colsurfb.2015.11.049cc-by-nc-nd (c) Elsevier B.V., 2015http://creativecommons.org/licenses/by-nc-nd/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/966222026-05-27T06:46:51Z
dc.title.none.fl_str_mv PEGylated and poloxamer-modified chitosan nanoparticles incorporating a lysine-based surfactant for pH-triggered doxorubicin release
title PEGylated and poloxamer-modified chitosan nanoparticles incorporating a lysine-based surfactant for pH-triggered doxorubicin release
spellingShingle PEGylated and poloxamer-modified chitosan nanoparticles incorporating a lysine-based surfactant for pH-triggered doxorubicin release
Scheeren, Laís E.
Medicaments antineoplàstics
Quitosan
Nanopartícules
Agents tensioactius
Sistemes d'alliberament de medicaments
Antineoplastic agents
Chitosan
Nanoparticles
Surface active agents
Drug delivery systems
title_short PEGylated and poloxamer-modified chitosan nanoparticles incorporating a lysine-based surfactant for pH-triggered doxorubicin release
title_full PEGylated and poloxamer-modified chitosan nanoparticles incorporating a lysine-based surfactant for pH-triggered doxorubicin release
title_fullStr PEGylated and poloxamer-modified chitosan nanoparticles incorporating a lysine-based surfactant for pH-triggered doxorubicin release
title_full_unstemmed PEGylated and poloxamer-modified chitosan nanoparticles incorporating a lysine-based surfactant for pH-triggered doxorubicin release
title_sort PEGylated and poloxamer-modified chitosan nanoparticles incorporating a lysine-based surfactant for pH-triggered doxorubicin release
dc.creator.none.fl_str_mv Scheeren, Laís E.
Nogueira, Daniele R.
Macedo, Letícia B.
Vinardell Martínez-Hidalgo, Ma. Pilar
Mitjans Arnal, Montserrat
Infante Martínez-Pardo, Ma. Rosa
Rolim, Clarice M. B.
author Scheeren, Laís E.
author_facet Scheeren, Laís E.
Nogueira, Daniele R.
Macedo, Letícia B.
Vinardell Martínez-Hidalgo, Ma. Pilar
Mitjans Arnal, Montserrat
Infante Martínez-Pardo, Ma. Rosa
Rolim, Clarice M. B.
author_role author
author2 Nogueira, Daniele R.
Macedo, Letícia B.
Vinardell Martínez-Hidalgo, Ma. Pilar
Mitjans Arnal, Montserrat
Infante Martínez-Pardo, Ma. Rosa
Rolim, Clarice M. B.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Medicaments antineoplàstics
Quitosan
Nanopartícules
Agents tensioactius
Sistemes d'alliberament de medicaments
Antineoplastic agents
Chitosan
Nanoparticles
Surface active agents
Drug delivery systems
topic Medicaments antineoplàstics
Quitosan
Nanopartícules
Agents tensioactius
Sistemes d'alliberament de medicaments
Antineoplastic agents
Chitosan
Nanoparticles
Surface active agents
Drug delivery systems
description The growing demand for efficient chemotherapy in many cancers requires novel approaches in target-delivery technologies. Nanomaterials with pH-responsive behavior appear to have potential ability to selectively release the encapsulated molecules by sensing the acidic tumor microenvironment or the low pH found in endosomes. Likewise, polyethylene glycol (PEG)- and poloxamer-modified nanocarriers have been gaining attention regarding their potential to improve the effectiveness of cancer therapy. In this context, DOX-loaded pH-responsive nanoparticles (NPs) modified with PEG or poloxamer were prepared and the effects of these modifiers were evaluated on the overall characteristics of these nanostructures. Chitosan and tripolyphosphate were selected to form NPs by the interaction of oppositely charged compounds. A pH-sensitive lysine-based amphiphile (77KS) was used as a bioactive adjuvant. The strong dependence of 77KS ionization with pH makes this compound an interesting candidate to be used for the design of pH-sensitive devices. The physicochemical characterization of all NPs has been performed, and it was shown that the presence of 77KS clearly promotes a pH-triggered DOX release. Accelerated and continuous release patterns of DOX from CS-NPs under acidic conditions were observed regardless of the presence of PEG or poloxamer. Moreover, photodegradation studies have indicated that the lyophilization of NPs improved DOX stability under UVA radiation. Finally, cytotoxicity experiments have shown the ability of DOX-loaded CS-NPs to kill HeLa tumor cells. Hence, the overall results suggest that these pH-responsive CS-NPs are highly potent delivery systems to target tumor and intracellular environments, rendering them promising DOX carrier systems for cancer therapy.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/96622
url https://hdl.handle.net/2445/96622
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: http://dx.doi.org/10.1016/j.colsurfb.2015.11.049
Colloids and Surfaces B-Biointerfaces, 2015, vol. 138, p. 117-127
http://dx.doi.org/10.1016/j.colsurfb.2015.11.049
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Elsevier B.V., 2015
http://creativecommons.org/licenses/by-nc-nd/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Elsevier B.V., 2015
http://creativecommons.org/licenses/by-nc-nd/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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