Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres
To create a chronic glaucoma animal model by a single intracameral injection of biodegradable poly lactic-co-glycolic acid (PLGA) microspheres (Ms) co-loaded with dexamethasone and fibronectin (MsDexaFibro). MsDexaFibro were prepared by a water-in-oil-in-water emulsion method including dexamethasone...
| Autores: | , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:269470 |
| Acceso en línea: | https://ddd.uab.cat/record/269470 https://dx.doi.org/urn:doi:10.1080/10717544.2022.2096712 |
| Access Level: | acceso abierto |
| Palabra clave: | Glaucoma animal model PLGA microspheres Multiloaded Intraocular pressure Neurodegeneration |
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Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheresAragón-Navas, Alba|||0000-0002-7466-8914Rodrigo Sanjuan, María Jesús|||0000-0002-4009-3075Garcia-Herranz, David|||0000-0003-2092-5927Martínez-Rincón, Teresa|||0000-0002-8891-0417Subías, Manuel|||0000-0002-2344-016XMendez-Martínez, Silvia|||0000-0001-6072-1545Ruberte Paris, Jesús|||0000-0003-1540-1432Pampalona Sala, Judit|||0000-0001-9454-0734Bravo-Osuna, Irene|||0000-0003-3133-7872Pablo, Luis E.|||0000-0003-2389-8282Garcia-Martin, Elena|||0000-0001-6258-2489Herrero-Vanrell, Rocío|||0000-0001-9764-7975Glaucoma animal modelPLGA microspheresMultiloadedIntraocular pressureNeurodegenerationTo create a chronic glaucoma animal model by a single intracameral injection of biodegradable poly lactic-co-glycolic acid (PLGA) microspheres (Ms) co-loaded with dexamethasone and fibronectin (MsDexaFibro). MsDexaFibro were prepared by a water-in-oil-in-water emulsion method including dexamethasone in the organic phase and fibronectin in the inner aqueous phase. To create the chronic glaucoma model, an interventionist and longitudinal animal study was performed using forty-five Long Evans rats (4-week-old). Rats received a single intracameral injection of MsDexafibro suspension (10%w/v) in the right eye. Ophthalmological parameters such as clinical signs, intraocular pressure (IOP), neuro-retinal functionality by electroretinography (ERG), retinal structural analysis by optical coherence tomography (OCT), and histology were evaluated up to six months. According to the results obtained, the model proposed was able to induce IOP increasing in both eyes over the study, higher in the injected eyes up to 6 weeks (p < 0.05), while preserving the ocular surface. OCT quantified progressive neuro-retinal degeneration (mainly in the retinal nerve fiber layer) in both eyes but higher in the injected eye. Ganglion cell functionality decreased in injected eyes, thus smaller amplitudes in PhNR were detected by ERG. In conclusion, a new chronic glaucoma animal model was created by a single injection of MsDexaFibro very similar to open-angle glaucoma occurring in humans. This model would impact in different fields such as ophthalmology, allowing long period of study of this pathology; pharmacology, evaluating the neuroprotective activity of active compounds; and pharmaceutical technology, allowing the correct evaluation of the efficacy of long-term sustained ocular drug delivery systems. 22022-01-0120222022-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/269470https://dx.doi.org/urn:doi:10.1080/10717544.2022.2096712reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengInstituto de Salud Carlos III https://doi.org/10.13039/501100004587 MAT2017-83858-C2-2Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 MAT2017-83858-C2-1Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 PRE2018-083951open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2694702026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres |
| title |
Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres |
| spellingShingle |
Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres Aragón-Navas, Alba|||0000-0002-7466-8914 Glaucoma animal model PLGA microspheres Multiloaded Intraocular pressure Neurodegeneration |
| title_short |
Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres |
| title_full |
Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres |
| title_fullStr |
Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres |
| title_full_unstemmed |
Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres |
| title_sort |
Mimicking chronic glaucoma over 6 months with a single intracameral injection of dexamethasone/fibronectin-loaded PLGA microspheres |
| dc.creator.none.fl_str_mv |
Aragón-Navas, Alba|||0000-0002-7466-8914 Rodrigo Sanjuan, María Jesús|||0000-0002-4009-3075 Garcia-Herranz, David|||0000-0003-2092-5927 Martínez-Rincón, Teresa|||0000-0002-8891-0417 Subías, Manuel|||0000-0002-2344-016X Mendez-Martínez, Silvia|||0000-0001-6072-1545 Ruberte Paris, Jesús|||0000-0003-1540-1432 Pampalona Sala, Judit|||0000-0001-9454-0734 Bravo-Osuna, Irene|||0000-0003-3133-7872 Pablo, Luis E.|||0000-0003-2389-8282 Garcia-Martin, Elena|||0000-0001-6258-2489 Herrero-Vanrell, Rocío|||0000-0001-9764-7975 |
| author |
Aragón-Navas, Alba|||0000-0002-7466-8914 |
| author_facet |
Aragón-Navas, Alba|||0000-0002-7466-8914 Rodrigo Sanjuan, María Jesús|||0000-0002-4009-3075 Garcia-Herranz, David|||0000-0003-2092-5927 Martínez-Rincón, Teresa|||0000-0002-8891-0417 Subías, Manuel|||0000-0002-2344-016X Mendez-Martínez, Silvia|||0000-0001-6072-1545 Ruberte Paris, Jesús|||0000-0003-1540-1432 Pampalona Sala, Judit|||0000-0001-9454-0734 Bravo-Osuna, Irene|||0000-0003-3133-7872 Pablo, Luis E.|||0000-0003-2389-8282 Garcia-Martin, Elena|||0000-0001-6258-2489 Herrero-Vanrell, Rocío|||0000-0001-9764-7975 |
| author_role |
author |
| author2 |
Rodrigo Sanjuan, María Jesús|||0000-0002-4009-3075 Garcia-Herranz, David|||0000-0003-2092-5927 Martínez-Rincón, Teresa|||0000-0002-8891-0417 Subías, Manuel|||0000-0002-2344-016X Mendez-Martínez, Silvia|||0000-0001-6072-1545 Ruberte Paris, Jesús|||0000-0003-1540-1432 Pampalona Sala, Judit|||0000-0001-9454-0734 Bravo-Osuna, Irene|||0000-0003-3133-7872 Pablo, Luis E.|||0000-0003-2389-8282 Garcia-Martin, Elena|||0000-0001-6258-2489 Herrero-Vanrell, Rocío|||0000-0001-9764-7975 |
| author2_role |
author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Glaucoma animal model PLGA microspheres Multiloaded Intraocular pressure Neurodegeneration |
| topic |
Glaucoma animal model PLGA microspheres Multiloaded Intraocular pressure Neurodegeneration |
| description |
To create a chronic glaucoma animal model by a single intracameral injection of biodegradable poly lactic-co-glycolic acid (PLGA) microspheres (Ms) co-loaded with dexamethasone and fibronectin (MsDexaFibro). MsDexaFibro were prepared by a water-in-oil-in-water emulsion method including dexamethasone in the organic phase and fibronectin in the inner aqueous phase. To create the chronic glaucoma model, an interventionist and longitudinal animal study was performed using forty-five Long Evans rats (4-week-old). Rats received a single intracameral injection of MsDexafibro suspension (10%w/v) in the right eye. Ophthalmological parameters such as clinical signs, intraocular pressure (IOP), neuro-retinal functionality by electroretinography (ERG), retinal structural analysis by optical coherence tomography (OCT), and histology were evaluated up to six months. According to the results obtained, the model proposed was able to induce IOP increasing in both eyes over the study, higher in the injected eyes up to 6 weeks (p < 0.05), while preserving the ocular surface. OCT quantified progressive neuro-retinal degeneration (mainly in the retinal nerve fiber layer) in both eyes but higher in the injected eye. Ganglion cell functionality decreased in injected eyes, thus smaller amplitudes in PhNR were detected by ERG. In conclusion, a new chronic glaucoma animal model was created by a single injection of MsDexaFibro very similar to open-angle glaucoma occurring in humans. This model would impact in different fields such as ophthalmology, allowing long period of study of this pathology; pharmacology, evaluating the neuroprotective activity of active compounds; and pharmaceutical technology, allowing the correct evaluation of the efficacy of long-term sustained ocular drug delivery systems. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2 2022-01-01 2022 2022-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/269470 https://dx.doi.org/urn:doi:10.1080/10717544.2022.2096712 |
| url |
https://ddd.uab.cat/record/269470 https://dx.doi.org/urn:doi:10.1080/10717544.2022.2096712 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 MAT2017-83858-C2-2 Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 MAT2017-83858-C2-1 Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 PRE2018-083951 |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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application/pdf |
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reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona |
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Universitat Autònoma de Barcelona |
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Dipòsit Digital de Documents de la UAB |
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Dipòsit Digital de Documents de la UAB |
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1869407746138308608 |
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15.300724 |