Sinorhizobium fredii HH103 RirA is required for oxidative stress resistance and efficient symbiosis with Soybean

Members of Rhizobiaceae contain a homologue of the iron-responsive regulatory protein RirA. In different bacteria, RirA acts as a repressor of iron uptake systems under iron-replete conditions and contributes to ameliorate cell damage during oxidative stress. In Rhizobium leguminosarum and Sinorhizo...

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Autores: Crespo Rivas, Juan Carlos, Navarro Gómez, Pilar, Alías Villegas, Cynthia, Moreno Onorato, Francisco Javier, Cubo Sánchez, María Teresa, Vinardell González, José María, Ruiz Sainz, José Enrique, Acosta Jurado, Sebastián
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/83783
Acceso en línea:https://hdl.handle.net/11441/83783
https://doi.org/10.3390/ijms20030787
Access Level:acceso abierto
Palabra clave:iron
Rhizobium
regulation
siderophore
nitrogen-fixation
plant–bacteria interaction
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repository_id_str
spelling Sinorhizobium fredii HH103 RirA is required for oxidative stress resistance and efficient symbiosis with SoybeanCrespo Rivas, Juan CarlosNavarro Gómez, PilarAlías Villegas, CynthiaMoreno Onorato, Francisco JavierCubo Sánchez, María TeresaVinardell González, José MaríaRuiz Sainz, José EnriqueAcosta Jurado, SebastiánironRhizobiumregulationsiderophorenitrogen-fixationplant–bacteria interactionMembers of Rhizobiaceae contain a homologue of the iron-responsive regulatory protein RirA. In different bacteria, RirA acts as a repressor of iron uptake systems under iron-replete conditions and contributes to ameliorate cell damage during oxidative stress. In Rhizobium leguminosarum and Sinorhizobium meliloti, mutations in rirA do not impair symbiotic nitrogen fixation. In this study, a rirA mutant of broad host range S. fredii HH103 has been constructed (SVQ780) and its free-living and symbiotic phenotypes evaluated. No production of siderophores could be detected in either the wild-type or SVQ780. The rirA mutant exhibited a growth advantage under iron-deficient conditions and hypersensitivity to hydrogen peroxide in iron-rich medium. Transcription of rirA in HH103 is subject to autoregulation and inactivation of the gene upregulates fbpA, a gene putatively involved in iron transport. The S. fredii rirA mutant was able to nodulate soybean plants, but symbiotic nitrogen fixation was impaired. Nodules induced by the mutant were poorly infected compared to those induced by the wild-type. Genetic complementation reversed the mutant’s hypersensitivity to H2O2, expression of fbpA, and symbiotic deficiency in soybean plants. This is the first report that demonstrates a role for RirA in the Rhizobium-legume symbiosis.Andalucian Government Grant No. P11-CVI-7500Spanish Government Grant Nos. BIO2013-42801-P and BIO2016-78409-REuropean Regional Development Funds (ERDF)VPPI (V Plan Propio de Investigación) of University of Seville.MDPIMicrobiologíaBiología Celular2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/83783https://doi.org/10.3390/ijms20030787reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésInternational Journal of Molecular Sciences, 20 (3), 787.P11-CVI-7500BIO2013-42801-PBIO2016-78409-Rhttps://doi.org/10.3390/ijms20030787info:eu-repo/semantics/openAccessoai:idus.us.es:11441/837832026-06-17T12:51:07Z
dc.title.none.fl_str_mv Sinorhizobium fredii HH103 RirA is required for oxidative stress resistance and efficient symbiosis with Soybean
title Sinorhizobium fredii HH103 RirA is required for oxidative stress resistance and efficient symbiosis with Soybean
spellingShingle Sinorhizobium fredii HH103 RirA is required for oxidative stress resistance and efficient symbiosis with Soybean
Crespo Rivas, Juan Carlos
iron
Rhizobium
regulation
siderophore
nitrogen-fixation
plant–bacteria interaction
title_short Sinorhizobium fredii HH103 RirA is required for oxidative stress resistance and efficient symbiosis with Soybean
title_full Sinorhizobium fredii HH103 RirA is required for oxidative stress resistance and efficient symbiosis with Soybean
title_fullStr Sinorhizobium fredii HH103 RirA is required for oxidative stress resistance and efficient symbiosis with Soybean
title_full_unstemmed Sinorhizobium fredii HH103 RirA is required for oxidative stress resistance and efficient symbiosis with Soybean
title_sort Sinorhizobium fredii HH103 RirA is required for oxidative stress resistance and efficient symbiosis with Soybean
dc.creator.none.fl_str_mv Crespo Rivas, Juan Carlos
Navarro Gómez, Pilar
Alías Villegas, Cynthia
Moreno Onorato, Francisco Javier
Cubo Sánchez, María Teresa
Vinardell González, José María
Ruiz Sainz, José Enrique
Acosta Jurado, Sebastián
author Crespo Rivas, Juan Carlos
author_facet Crespo Rivas, Juan Carlos
Navarro Gómez, Pilar
Alías Villegas, Cynthia
Moreno Onorato, Francisco Javier
Cubo Sánchez, María Teresa
Vinardell González, José María
Ruiz Sainz, José Enrique
Acosta Jurado, Sebastián
author_role author
author2 Navarro Gómez, Pilar
Alías Villegas, Cynthia
Moreno Onorato, Francisco Javier
Cubo Sánchez, María Teresa
Vinardell González, José María
Ruiz Sainz, José Enrique
Acosta Jurado, Sebastián
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Microbiología
Biología Celular
dc.subject.none.fl_str_mv iron
Rhizobium
regulation
siderophore
nitrogen-fixation
plant–bacteria interaction
topic iron
Rhizobium
regulation
siderophore
nitrogen-fixation
plant–bacteria interaction
description Members of Rhizobiaceae contain a homologue of the iron-responsive regulatory protein RirA. In different bacteria, RirA acts as a repressor of iron uptake systems under iron-replete conditions and contributes to ameliorate cell damage during oxidative stress. In Rhizobium leguminosarum and Sinorhizobium meliloti, mutations in rirA do not impair symbiotic nitrogen fixation. In this study, a rirA mutant of broad host range S. fredii HH103 has been constructed (SVQ780) and its free-living and symbiotic phenotypes evaluated. No production of siderophores could be detected in either the wild-type or SVQ780. The rirA mutant exhibited a growth advantage under iron-deficient conditions and hypersensitivity to hydrogen peroxide in iron-rich medium. Transcription of rirA in HH103 is subject to autoregulation and inactivation of the gene upregulates fbpA, a gene putatively involved in iron transport. The S. fredii rirA mutant was able to nodulate soybean plants, but symbiotic nitrogen fixation was impaired. Nodules induced by the mutant were poorly infected compared to those induced by the wild-type. Genetic complementation reversed the mutant’s hypersensitivity to H2O2, expression of fbpA, and symbiotic deficiency in soybean plants. This is the first report that demonstrates a role for RirA in the Rhizobium-legume symbiosis.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/83783
https://doi.org/10.3390/ijms20030787
url https://hdl.handle.net/11441/83783
https://doi.org/10.3390/ijms20030787
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv International Journal of Molecular Sciences, 20 (3), 787.
P11-CVI-7500
BIO2013-42801-P
BIO2016-78409-R
https://doi.org/10.3390/ijms20030787
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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