The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection

Throughout their life cycle, pathogens are challenged by Reactive Oxygen Species (ROS) and must deploy defence mechanisms against oxidative damage. Environmental stressors such as ultraviolet radiation and desiccation induce intracellular ROS production. Moreover, during pathogen colonisation, plant...

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Autores: Corral, Jordi, Rocafort, Mercedes, Ripa, Maria-belén, Vandecaveye, Agustina, Pezzoni, Magdalena, Invernon, Alicia, Coll, Núria S., Valls i Matheu, Marc, Orellano, Elena
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/227137
Acceso en línea:https://hdl.handle.net/2445/227137
Access Level:acceso abierto
Palabra clave:Microorganismes patògens
Esforç i tensió
Plantes
Pathogenic microorganisms
Strains and stresses
Plants
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spelling The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infectionCorral, JordiRocafort, MercedesRipa, Maria-belénVandecaveye, AgustinaPezzoni, MagdalenaInvernon, AliciaColl, Núria S.Valls i Matheu, MarcOrellano, ElenaMicroorganismes patògensEsforç i tensióPlantesPathogenic microorganismsStrains and stressesPlantsThroughout their life cycle, pathogens are challenged by Reactive Oxygen Species (ROS) and must deploy defence mechanisms against oxidative damage. Environmental stressors such as ultraviolet radiation and desiccation induce intracellular ROS production. Moreover, during pathogen colonisation, plant hosts initiate an oxidative burst, leading to the accumulation of hydrogen peroxide (H2O2). Catalases mitigate ROS toxicity by catalysing the breakdown of H2O2 into water and oxygen. In this study, we explored the roles of the monofunctional catalase (KatE) and the bifunctional catalase-peroxidase (KatG) in the phytopathogen Ralstonia solanacearum during both environmental stress conditions and in planta colonisation stages. Our results demonstrate that both catalases are critical for surviving ROS, with KatG having a more preponderant role. Plant pathogenicity assays show that catalases are not required for virulence although they are important for bacterial fitness in the apoplast. In contrast, we prove that both catalases are vital for R. solanacearum response to oxidative stress in the external environment. KatG was indispensable for survival in soil, as well as for enduring ultraviolet radiation and desiccation exposure. Our findings reveal that the primary function of R. solanacearum catalases is to counteract ROS generated by environmental stressors rather than host-derived oxidative defences. This research identifies specific environmental stressors that these catalases combat during pathogen survival. These insights pave the way for future strategies targeting catalase activity to control bacterial wilt disease.Nature Publishing Group2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/227137Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1038/s41598-025-19174-5Scientific Reports, 2025, vol. 15, num.1, p. 1-14https://doi.org/10.1038/s41598-025-19174-5cc-by-nc-nd (c) Corral J et al., 2025http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2271372026-05-27T06:46:51Z
dc.title.none.fl_str_mv The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection
title The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection
spellingShingle The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection
Corral, Jordi
Microorganismes patògens
Esforç i tensió
Plantes
Pathogenic microorganisms
Strains and stresses
Plants
title_short The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection
title_full The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection
title_fullStr The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection
title_full_unstemmed The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection
title_sort The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection
dc.creator.none.fl_str_mv Corral, Jordi
Rocafort, Mercedes
Ripa, Maria-belén
Vandecaveye, Agustina
Pezzoni, Magdalena
Invernon, Alicia
Coll, Núria S.
Valls i Matheu, Marc
Orellano, Elena
author Corral, Jordi
author_facet Corral, Jordi
Rocafort, Mercedes
Ripa, Maria-belén
Vandecaveye, Agustina
Pezzoni, Magdalena
Invernon, Alicia
Coll, Núria S.
Valls i Matheu, Marc
Orellano, Elena
author_role author
author2 Rocafort, Mercedes
Ripa, Maria-belén
Vandecaveye, Agustina
Pezzoni, Magdalena
Invernon, Alicia
Coll, Núria S.
Valls i Matheu, Marc
Orellano, Elena
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Microorganismes patògens
Esforç i tensió
Plantes
Pathogenic microorganisms
Strains and stresses
Plants
topic Microorganismes patògens
Esforç i tensió
Plantes
Pathogenic microorganisms
Strains and stresses
Plants
description Throughout their life cycle, pathogens are challenged by Reactive Oxygen Species (ROS) and must deploy defence mechanisms against oxidative damage. Environmental stressors such as ultraviolet radiation and desiccation induce intracellular ROS production. Moreover, during pathogen colonisation, plant hosts initiate an oxidative burst, leading to the accumulation of hydrogen peroxide (H2O2). Catalases mitigate ROS toxicity by catalysing the breakdown of H2O2 into water and oxygen. In this study, we explored the roles of the monofunctional catalase (KatE) and the bifunctional catalase-peroxidase (KatG) in the phytopathogen Ralstonia solanacearum during both environmental stress conditions and in planta colonisation stages. Our results demonstrate that both catalases are critical for surviving ROS, with KatG having a more preponderant role. Plant pathogenicity assays show that catalases are not required for virulence although they are important for bacterial fitness in the apoplast. In contrast, we prove that both catalases are vital for R. solanacearum response to oxidative stress in the external environment. KatG was indispensable for survival in soil, as well as for enduring ultraviolet radiation and desiccation exposure. Our findings reveal that the primary function of R. solanacearum catalases is to counteract ROS generated by environmental stressors rather than host-derived oxidative defences. This research identifies specific environmental stressors that these catalases combat during pathogen survival. These insights pave the way for future strategies targeting catalase activity to control bacterial wilt disease.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/227137
url https://hdl.handle.net/2445/227137
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1038/s41598-025-19174-5
Scientific Reports, 2025, vol. 15, num.1, p. 1-14
https://doi.org/10.1038/s41598-025-19174-5
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Corral J et al., 2025
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Corral J et al., 2025
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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