Structural and functional analysis of polyethylene glycol-coated zein nanoparticles

The development of nanoparticles for drug delivery purposes is faced to a number of challenges, including the complexity of an adequate physico-chemical characterization, especially when nanoparticles are functionalized. The aim of this work was to evaluate and characterize the structure and propert...

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Autores: Calvopiña, Jonathan, Rivero Fuente, Pedro J., Martínez-Ohárriz, María Cristina, Palacio, José F., Álvarez Galindo, José Ignacio, Espuelas, Socorro, Irache, Juan M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/54339
Acceso en línea:https://hdl.handle.net/2454/54339
Access Level:acceso abierto
Palabra clave:Zein
Nanoparticles
Polyethylene glycol
Characterization
Thin film
Mucin
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spelling Structural and functional analysis of polyethylene glycol-coated zein nanoparticlesCalvopiña, JonathanRivero Fuente, Pedro J.Martínez-Ohárriz, María CristinaPalacio, José F.Álvarez Galindo, José IgnacioEspuelas, SocorroIrache, Juan M. ZeinNanoparticlesPolyethylene glycolCharacterizationThin filmMucinThe development of nanoparticles for drug delivery purposes is faced to a number of challenges, including the complexity of an adequate physico-chemical characterization, especially when nanoparticles are functionalized. The aim of this work was to evaluate and characterize the structure and properties of zein nanoparticles (approximately 200 nm) functionalized with polyethylene glycol (PEG 35,000) at different PEG-to-zein ratios. The interaction between PEG and zein, attributed to hydrogen bonding between the amide groups of zein and the hydroxyl groups of PEG, was evidenced by FTIR and further confirmed by 2D-COS spectroscopy. DSC and TGA thermograms demonstrated the role of PEG in improving nanoparticle thermal stability, with PEG-coated nanoparticles exhibiting higher decomposition temperatures and increased PEG content with higher PEG-to-zein ratios. SEM and AFM imaging of nanoparticle thin films confirmed that PEG decoration enhanced wettability and reduced the hydrophobicity of bare zein nanoparticles, consistent with Rose Bengal test results. Additionally, AFM analysis of nanoparticle distribution in a mucin thin film demonstrated that PEG coating improved nanoparticle dispersibility within the mucin matrix, in line with previous studies describing the increased diffusivity and mucus-permeation properties of nanoparticles coated with hydrophilic polymers. In summary, this study highlights the importance of an in-depth analysis of the physicochemical characteristics of nanoparticles, providing valuable structural insights that facilitate optimization and the selection of the most suitable compositions for preclinical development.ElsevierIngenieríaIngeniaritzaInstitute for Advanced Materials and Mathematics - INAMAT22025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2454/54339reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglés© 2025 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license.http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/543392026-06-17T12:41:47Z
dc.title.none.fl_str_mv Structural and functional analysis of polyethylene glycol-coated zein nanoparticles
title Structural and functional analysis of polyethylene glycol-coated zein nanoparticles
spellingShingle Structural and functional analysis of polyethylene glycol-coated zein nanoparticles
Calvopiña, Jonathan
Zein
Nanoparticles
Polyethylene glycol
Characterization
Thin film
Mucin
title_short Structural and functional analysis of polyethylene glycol-coated zein nanoparticles
title_full Structural and functional analysis of polyethylene glycol-coated zein nanoparticles
title_fullStr Structural and functional analysis of polyethylene glycol-coated zein nanoparticles
title_full_unstemmed Structural and functional analysis of polyethylene glycol-coated zein nanoparticles
title_sort Structural and functional analysis of polyethylene glycol-coated zein nanoparticles
dc.creator.none.fl_str_mv Calvopiña, Jonathan
Rivero Fuente, Pedro J.
Martínez-Ohárriz, María Cristina
Palacio, José F.
Álvarez Galindo, José Ignacio
Espuelas, Socorro
Irache, Juan M.
author Calvopiña, Jonathan
author_facet Calvopiña, Jonathan
Rivero Fuente, Pedro J.
Martínez-Ohárriz, María Cristina
Palacio, José F.
Álvarez Galindo, José Ignacio
Espuelas, Socorro
Irache, Juan M.
author_role author
author2 Rivero Fuente, Pedro J.
Martínez-Ohárriz, María Cristina
Palacio, José F.
Álvarez Galindo, José Ignacio
Espuelas, Socorro
Irache, Juan M.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ingeniería
Ingeniaritza
Institute for Advanced Materials and Mathematics - INAMAT2
dc.subject.none.fl_str_mv Zein
Nanoparticles
Polyethylene glycol
Characterization
Thin film
Mucin
topic Zein
Nanoparticles
Polyethylene glycol
Characterization
Thin film
Mucin
description The development of nanoparticles for drug delivery purposes is faced to a number of challenges, including the complexity of an adequate physico-chemical characterization, especially when nanoparticles are functionalized. The aim of this work was to evaluate and characterize the structure and properties of zein nanoparticles (approximately 200 nm) functionalized with polyethylene glycol (PEG 35,000) at different PEG-to-zein ratios. The interaction between PEG and zein, attributed to hydrogen bonding between the amide groups of zein and the hydroxyl groups of PEG, was evidenced by FTIR and further confirmed by 2D-COS spectroscopy. DSC and TGA thermograms demonstrated the role of PEG in improving nanoparticle thermal stability, with PEG-coated nanoparticles exhibiting higher decomposition temperatures and increased PEG content with higher PEG-to-zein ratios. SEM and AFM imaging of nanoparticle thin films confirmed that PEG decoration enhanced wettability and reduced the hydrophobicity of bare zein nanoparticles, consistent with Rose Bengal test results. Additionally, AFM analysis of nanoparticle distribution in a mucin thin film demonstrated that PEG coating improved nanoparticle dispersibility within the mucin matrix, in line with previous studies describing the increased diffusivity and mucus-permeation properties of nanoparticles coated with hydrophilic polymers. In summary, this study highlights the importance of an in-depth analysis of the physicochemical characteristics of nanoparticles, providing valuable structural insights that facilitate optimization and the selection of the most suitable compositions for preclinical development.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/54339
url https://hdl.handle.net/2454/54339
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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