Exploiting multiSite Gateway and penNFRUIT plasmid collection for fruit genetic engineering

[EN] MultiSite Gateway cloning techniques based on homologous recombination facilitate the combinatorial assembly of basic genetic pieces (i.e., promoters, CDS, and terminators) into gene expression or gene silencing cassettes. pENFRUIT is a collection of MultiSite Triple Gateway Entry vectors dedic...

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Autores: Hueso Estornell, Leandro, GRANELL RICHART, ANTONIO|||0000-0003-4266-9581, Orzáez Calatayud, Diego Vicente
Tipo de recurso: artículo
Fecha de publicación:2012
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/83432
Acceso en línea:https://riunet.upv.es/handle/10251/83432
Access Level:acceso abierto
Palabra clave:Gateway cloning
Gene expression
Gene silencing
HpRNAi
Transient expression
Agroinjection
BIOQUIMICA Y BIOLOGIA MOLECULAR
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spelling Exploiting multiSite Gateway and penNFRUIT plasmid collection for fruit genetic engineeringHueso Estornell, LeandroGRANELL RICHART, ANTONIO|||0000-0003-4266-9581Orzáez Calatayud, Diego VicenteGateway cloningGene expressionGene silencingHpRNAiTransient expressionAgroinjectionBIOQUIMICA Y BIOLOGIA MOLECULAR[EN] MultiSite Gateway cloning techniques based on homologous recombination facilitate the combinatorial assembly of basic genetic pieces (i.e., promoters, CDS, and terminators) into gene expression or gene silencing cassettes. pENFRUIT is a collection of MultiSite Triple Gateway Entry vectors dedicated to genetic engineering in fruits. It comprises a number of fruit-operating promoters as well as C-terminal tags adapted to the Gateway standard. In this way, flanking regulatory/labeling sequences can be easily Gateway-assembled with a given gene of interest for its ectopic expression or silencing in fruits. The resulting gene constructs can be analyzed in stable transgenic plants or in transient expression assays, the latter allowing fast testing of the increasing number of combinations arising from MultiSite methodology. A detailed description of the use of MultiSite cloning methodology for the assembly of pENFRUIT elements is presented.This work was supported by the Spanish Ministry of Science and Education (Ramón y Cajal program, BIO2010-15384 and BIO2008-03434 projects), the European Commission (program I3P and EU-SOL project), and Fundación Genoma España (ESP-SOL project).Humana PressInstituto Universitario Mixto de Biología Molecular y Celular de PlantasMinisterio de Ciencia e InnovaciónMinisterio de Educación y CienciaRepositorio Institucional de la Universitat Politècnica de València Riunet20122012-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/83432reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Educación y Ciencia https://doi.org/10.13039/501100008743 BIO2005-01015 PRODUCCION DE PROTEINAS INMUNOTERAPEUTICAS DE APLICACION ORAL EN FRUTOS DE TOMATEEuropean Commission https://doi.org/10.13039/501100000780 FP7 268465 Real-time experimental study of pattern selection dynamics in eutectic solidificationMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BIO2008-03434 EL FRUTO DE TOMATE COMO BIOFACTORIA DE PROTEINAS INMUNOTERAPEUTICAS ORALES: PRODUCCION DE ANTICUERPOS FRENTE A ROTAVIRUSopen accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/834322026-06-13T07:49:27Z
dc.title.none.fl_str_mv Exploiting multiSite Gateway and penNFRUIT plasmid collection for fruit genetic engineering
title Exploiting multiSite Gateway and penNFRUIT plasmid collection for fruit genetic engineering
spellingShingle Exploiting multiSite Gateway and penNFRUIT plasmid collection for fruit genetic engineering
Hueso Estornell, Leandro
Gateway cloning
Gene expression
Gene silencing
HpRNAi
Transient expression
Agroinjection
BIOQUIMICA Y BIOLOGIA MOLECULAR
title_short Exploiting multiSite Gateway and penNFRUIT plasmid collection for fruit genetic engineering
title_full Exploiting multiSite Gateway and penNFRUIT plasmid collection for fruit genetic engineering
title_fullStr Exploiting multiSite Gateway and penNFRUIT plasmid collection for fruit genetic engineering
title_full_unstemmed Exploiting multiSite Gateway and penNFRUIT plasmid collection for fruit genetic engineering
title_sort Exploiting multiSite Gateway and penNFRUIT plasmid collection for fruit genetic engineering
dc.creator.none.fl_str_mv Hueso Estornell, Leandro
GRANELL RICHART, ANTONIO|||0000-0003-4266-9581
Orzáez Calatayud, Diego Vicente
author Hueso Estornell, Leandro
author_facet Hueso Estornell, Leandro
GRANELL RICHART, ANTONIO|||0000-0003-4266-9581
Orzáez Calatayud, Diego Vicente
author_role author
author2 GRANELL RICHART, ANTONIO|||0000-0003-4266-9581
Orzáez Calatayud, Diego Vicente
author2_role author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
Ministerio de Ciencia e Innovación
Ministerio de Educación y Ciencia
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Gateway cloning
Gene expression
Gene silencing
HpRNAi
Transient expression
Agroinjection
BIOQUIMICA Y BIOLOGIA MOLECULAR
topic Gateway cloning
Gene expression
Gene silencing
HpRNAi
Transient expression
Agroinjection
BIOQUIMICA Y BIOLOGIA MOLECULAR
description [EN] MultiSite Gateway cloning techniques based on homologous recombination facilitate the combinatorial assembly of basic genetic pieces (i.e., promoters, CDS, and terminators) into gene expression or gene silencing cassettes. pENFRUIT is a collection of MultiSite Triple Gateway Entry vectors dedicated to genetic engineering in fruits. It comprises a number of fruit-operating promoters as well as C-terminal tags adapted to the Gateway standard. In this way, flanking regulatory/labeling sequences can be easily Gateway-assembled with a given gene of interest for its ectopic expression or silencing in fruits. The resulting gene constructs can be analyzed in stable transgenic plants or in transient expression assays, the latter allowing fast testing of the increasing number of combinations arising from MultiSite methodology. A detailed description of the use of MultiSite cloning methodology for the assembly of pENFRUIT elements is presented.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/83432
url https://riunet.upv.es/handle/10251/83432
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Educación y Ciencia https://doi.org/10.13039/501100008743 BIO2005-01015 PRODUCCION DE PROTEINAS INMUNOTERAPEUTICAS DE APLICACION ORAL EN FRUTOS DE TOMATE
European Commission https://doi.org/10.13039/501100000780 FP7 268465 Real-time experimental study of pattern selection dynamics in eutectic solidification
Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BIO2008-03434 EL FRUTO DE TOMATE COMO BIOFACTORIA DE PROTEINAS INMUNOTERAPEUTICAS ORALES: PRODUCCION DE ANTICUERPOS FRENTE A ROTAVIRUS
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Humana Press
publisher.none.fl_str_mv Humana Press
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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