Identification of a newly conserved SLA-II epitope in a structural protein of swine influenza virus

Despite the role of pigs as a source of new Influenza A Virus viruses (IAV) potentially capable of initiating human pandemics, immune responses to swine influenza virus (SwIV) in pigs are not fully understood. Several SwIV epitopes presented by swine MHC (SLA) class I have been identified using diff...

Descripción completa

Detalles Bibliográficos
Autores: Baratelli, Massimiliano, Morgan, Sophie, Hemmink, Johanneke Dinie, Reid, Elisabeth, Carr, Brigid Veronica, Lefevre, Eric, Montaner Tarbes, Sergio Roberto, Charleston, Bryan, Fraile Sauce, Lorenzo José, Tchilian, Elma, Montoya, Maria
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10459.1/69571
Acceso en línea:https://doi.org/10.3389/fimmu.2020.02083
http://hdl.handle.net/10459.1/69571
Access Level:acceso abierto
Palabra clave:Swine influenza
Class II epitopes
Swine
Nucleoprotein
id ES_4ddbca5cefb36f8eaa5e2c06eb79b4ef
oai_identifier_str oai:recercat.cat:10459.1/69571
network_acronym_str ES
network_name_str España
repository_id_str
spelling Identification of a newly conserved SLA-II epitope in a structural protein of swine influenza virusBaratelli, MassimilianoMorgan, SophieHemmink, Johanneke DinieReid, ElisabethCarr, Brigid VeronicaLefevre, EricMontaner Tarbes, Sergio RobertoCharleston, BryanFraile Sauce, Lorenzo JoséTchilian, ElmaMontoya, MariaSwine influenzaClass II epitopesSwineNucleoproteinDespite the role of pigs as a source of new Influenza A Virus viruses (IAV) potentially capable of initiating human pandemics, immune responses to swine influenza virus (SwIV) in pigs are not fully understood. Several SwIV epitopes presented by swine MHC (SLA) class I have been identified using different approaches either in outbred pigs or in Babraham large white inbred pigs, which are 85% identical by genome wide SNP analysis. On the other hand, some class II SLA epitopes were recently described in outbred pigs. In this work, Babraham large white inbred pigs were selected to identify SLA II epitopes from SwIV H1N1. PBMCs were screened for recognition of overlapping peptides covering the NP and M1 proteins from heterologous IAV H1N1 in IFNγ ELISPOT. A novel SLA class II restricted epitope was identified in NP from swine H1N1. This conserved novel epitope could be the base for further vaccine approaches against H1N1 in pigs.The research leading to these results has received funding from the European Community's Seventh Framework Program (FP7, 2007-2013), the Research Infrastructures Action under grant FP7-228393 (a NADIR project); from the project AGL2010-22200-C02-01 from the Spanish Ministry of Science and Innovation; the UK's BBSRC grant BBS/E/I/00002014.Frontiers Media2020202020202020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.3389/fimmu.2020.02083http://hdl.handle.net/10459.1/69571http://hdl.handle.net/10459.1/69571reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/grantAgreement/MICINN//AGL2010-22200-C02-01Reproducció del document publicat a: https://doi.org/10.3389/fimmu.2020.02083Frontiers In Immunology, 2020, vol. 11, p. 2083info:eu-repo/grantAgreement/EC/FP7/228394cc-by (c) Baratelli, Massimiliano et al., 2020info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/4.0/oai:recercat.cat:10459.1/695712026-05-29T05:05:01Z
dc.title.none.fl_str_mv Identification of a newly conserved SLA-II epitope in a structural protein of swine influenza virus
title Identification of a newly conserved SLA-II epitope in a structural protein of swine influenza virus
spellingShingle Identification of a newly conserved SLA-II epitope in a structural protein of swine influenza virus
Baratelli, Massimiliano
Swine influenza
Class II epitopes
Swine
Nucleoprotein
title_short Identification of a newly conserved SLA-II epitope in a structural protein of swine influenza virus
title_full Identification of a newly conserved SLA-II epitope in a structural protein of swine influenza virus
title_fullStr Identification of a newly conserved SLA-II epitope in a structural protein of swine influenza virus
title_full_unstemmed Identification of a newly conserved SLA-II epitope in a structural protein of swine influenza virus
title_sort Identification of a newly conserved SLA-II epitope in a structural protein of swine influenza virus
dc.creator.none.fl_str_mv Baratelli, Massimiliano
Morgan, Sophie
Hemmink, Johanneke Dinie
Reid, Elisabeth
Carr, Brigid Veronica
Lefevre, Eric
Montaner Tarbes, Sergio Roberto
Charleston, Bryan
Fraile Sauce, Lorenzo José
Tchilian, Elma
Montoya, Maria
author Baratelli, Massimiliano
author_facet Baratelli, Massimiliano
Morgan, Sophie
Hemmink, Johanneke Dinie
Reid, Elisabeth
Carr, Brigid Veronica
Lefevre, Eric
Montaner Tarbes, Sergio Roberto
Charleston, Bryan
Fraile Sauce, Lorenzo José
Tchilian, Elma
Montoya, Maria
author_role author
author2 Morgan, Sophie
Hemmink, Johanneke Dinie
Reid, Elisabeth
Carr, Brigid Veronica
Lefevre, Eric
Montaner Tarbes, Sergio Roberto
Charleston, Bryan
Fraile Sauce, Lorenzo José
Tchilian, Elma
Montoya, Maria
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Swine influenza
Class II epitopes
Swine
Nucleoprotein
topic Swine influenza
Class II epitopes
Swine
Nucleoprotein
description Despite the role of pigs as a source of new Influenza A Virus viruses (IAV) potentially capable of initiating human pandemics, immune responses to swine influenza virus (SwIV) in pigs are not fully understood. Several SwIV epitopes presented by swine MHC (SLA) class I have been identified using different approaches either in outbred pigs or in Babraham large white inbred pigs, which are 85% identical by genome wide SNP analysis. On the other hand, some class II SLA epitopes were recently described in outbred pigs. In this work, Babraham large white inbred pigs were selected to identify SLA II epitopes from SwIV H1N1. PBMCs were screened for recognition of overlapping peptides covering the NP and M1 proteins from heterologous IAV H1N1 in IFNγ ELISPOT. A novel SLA class II restricted epitope was identified in NP from swine H1N1. This conserved novel epitope could be the base for further vaccine approaches against H1N1 in pigs.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.3389/fimmu.2020.02083
http://hdl.handle.net/10459.1/69571
http://hdl.handle.net/10459.1/69571
url https://doi.org/10.3389/fimmu.2020.02083
http://hdl.handle.net/10459.1/69571
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICINN//AGL2010-22200-C02-01
Reproducció del document publicat a: https://doi.org/10.3389/fimmu.2020.02083
Frontiers In Immunology, 2020, vol. 11, p. 2083
info:eu-repo/grantAgreement/EC/FP7/228394
dc.rights.none.fl_str_mv cc-by (c) Baratelli, Massimiliano et al., 2020
info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c) Baratelli, Massimiliano et al., 2020
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869407719428980736
score 15.812455

Similares en LA Referencia