The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles

The floral perianth, comprising sepals and petals, conceals the sexual organs and attracts pollinators. The coordination of growth and scent emission is not fully understood. We have analyzed the effect of knocking down CHANEL (PhCHL), the ZEITLUPE ortholog in petunia (PhCHL) by hairpin RNAs. Plants...

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Autores: Terry López, Marta Isabel, Pérez Sanz, Fernando, Díaz Galián, María Victoria, Pérez de los Cobos Arnao, Felipe, Navarro, Pedro J., Egea Gutiérrez-Cortines, Marcos, Weiss, Julia Rosl
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Universidad Politécnica de Cartagena(UPCT)
Repositorio:Repositorio Digital UPCT
OAI Identifier:oai:repositorio.upct.es:10317/9631
Acceso en línea:http://hdl.handle.net/10317/9631
Access Level:acceso abierto
Palabra clave:Floral scent
Petal development
Growth rate
Phenomics
Circadian clock
ZEITLUPE
Image analysis
Hairpin RNA
Botánica
Producción Vegetal
2407 Biología Celular
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spelling The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent ProfilesTerry López, Marta IsabelPérez Sanz, FernandoDíaz Galián, María VictoriaPérez de los Cobos Arnao, FelipeNavarro, Pedro J.Egea Gutiérrez-Cortines, MarcosWeiss, Julia RoslFloral scentPetal developmentGrowth ratePhenomicsCircadian clockZEITLUPEImage analysisHairpin RNABotánicaProducción Vegetal2407 Biología CelularThe floral perianth, comprising sepals and petals, conceals the sexual organs and attracts pollinators. The coordination of growth and scent emission is not fully understood. We have analyzed the effect of knocking down CHANEL (PhCHL), the ZEITLUPE ortholog in petunia (PhCHL) by hairpin RNAs. Plants with low PhCHL mRNA had overall decreased size. Growth evaluation using time lapse image analysis showed that early leaf movement was not affected by RNAi:PhCHL, but flower angle movement was modified, moving earlier during the day in knockdown plants than in wild types. Despite differences in stem length, growth rate was not significantly affected by loss of PhCHL. In contrast, petal growth displayed lower growth rate in RNAi:PhCHL. Decreased levels of PhCHL caused strongly modified scent profiles, including changes in composition and timing of emission resulting in volatile profiles highly divergent from the wild type. Our results show a role of PhCHL in controlling growth and development of vegetative and reproductive organs in petunia. The different effects of PhCHL on organ development indicate an organ-specific interpretation of the down regulation of PhCHL. Through the control of both timing and quantitative volatile emissions, PhCHL appears to be a major coordinator of scent profiles.This research was funded by Fundacion Seneca 19398/PI/14, 19895/GERM/15 and MC BFU-2017 88300-C2-1-R and BFU-2017 88300-C2-2-R. We would like to acknowledge María José Roca for technical assistance.MDPIUniversidad Politécnica de CartagenaUniversidad de Murcia202120212019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10317/9631reponame:Repositorio Digital UPCTinstname:Universidad Politécnica de Cartagena(UPCT)Ingléshttps://www.mdpi.com/2073-4409/8/4/343Atribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:repositorio.upct.es:10317/96312026-05-15T06:39:02Z
dc.title.none.fl_str_mv The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles
title The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles
spellingShingle The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles
Terry López, Marta Isabel
Floral scent
Petal development
Growth rate
Phenomics
Circadian clock
ZEITLUPE
Image analysis
Hairpin RNA
Botánica
Producción Vegetal
2407 Biología Celular
title_short The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles
title_full The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles
title_fullStr The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles
title_full_unstemmed The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles
title_sort The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles
dc.creator.none.fl_str_mv Terry López, Marta Isabel
Pérez Sanz, Fernando
Díaz Galián, María Victoria
Pérez de los Cobos Arnao, Felipe
Navarro, Pedro J.
Egea Gutiérrez-Cortines, Marcos
Weiss, Julia Rosl
author Terry López, Marta Isabel
author_facet Terry López, Marta Isabel
Pérez Sanz, Fernando
Díaz Galián, María Victoria
Pérez de los Cobos Arnao, Felipe
Navarro, Pedro J.
Egea Gutiérrez-Cortines, Marcos
Weiss, Julia Rosl
author_role author
author2 Pérez Sanz, Fernando
Díaz Galián, María Victoria
Pérez de los Cobos Arnao, Felipe
Navarro, Pedro J.
Egea Gutiérrez-Cortines, Marcos
Weiss, Julia Rosl
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Politécnica de Cartagena
Universidad de Murcia
dc.subject.none.fl_str_mv Floral scent
Petal development
Growth rate
Phenomics
Circadian clock
ZEITLUPE
Image analysis
Hairpin RNA
Botánica
Producción Vegetal
2407 Biología Celular
topic Floral scent
Petal development
Growth rate
Phenomics
Circadian clock
ZEITLUPE
Image analysis
Hairpin RNA
Botánica
Producción Vegetal
2407 Biología Celular
description The floral perianth, comprising sepals and petals, conceals the sexual organs and attracts pollinators. The coordination of growth and scent emission is not fully understood. We have analyzed the effect of knocking down CHANEL (PhCHL), the ZEITLUPE ortholog in petunia (PhCHL) by hairpin RNAs. Plants with low PhCHL mRNA had overall decreased size. Growth evaluation using time lapse image analysis showed that early leaf movement was not affected by RNAi:PhCHL, but flower angle movement was modified, moving earlier during the day in knockdown plants than in wild types. Despite differences in stem length, growth rate was not significantly affected by loss of PhCHL. In contrast, petal growth displayed lower growth rate in RNAi:PhCHL. Decreased levels of PhCHL caused strongly modified scent profiles, including changes in composition and timing of emission resulting in volatile profiles highly divergent from the wild type. Our results show a role of PhCHL in controlling growth and development of vegetative and reproductive organs in petunia. The different effects of PhCHL on organ development indicate an organ-specific interpretation of the down regulation of PhCHL. Through the control of both timing and quantitative volatile emissions, PhCHL appears to be a major coordinator of scent profiles.
publishDate 2019
dc.date.none.fl_str_mv 2019
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10317/9631
url http://hdl.handle.net/10317/9631
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.mdpi.com/2073-4409/8/4/343
dc.rights.none.fl_str_mv Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositorio Digital UPCT
instname:Universidad Politécnica de Cartagena(UPCT)
instname_str Universidad Politécnica de Cartagena(UPCT)
reponame_str Repositorio Digital UPCT
collection Repositorio Digital UPCT
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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