A bivalent dendrimeric peptide bearing a T-cell epitope from foot-and-mouth disease virus protein 3A improves humoral response against classical swine fever virus

Three dendrimeric peptides were synthesized in order to evaluate their immunogenicity and their potential protection against classical swine fever virus (CSFV) in domestic pigs. Construct 1, an optimized version of a previously used dendrimer, had four copies of a B-cell epitope derived from CSFV E2...

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Authors: Bohórquez, José Alejandro, Defaus, Sira, Muñoz González, Sara, Perez-Simó, Marta, Rosell, Rosa, Fraile Sauce, Lorenzo José, Sobrino, Francisco, Andreu, David, Ganges, Llilianne
Format: article
Status:Versión aceptada para publicación
Publication Date:2017
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10459.1/64676
Online Access:https://doi.org/10.1016/j.virusres.2017.05.020
http://hdl.handle.net/10459.1/64676
Access Level:Open access
Keyword:B cell epitope
CSFV
Dendrimeric peptide
FMDV
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spelling A bivalent dendrimeric peptide bearing a T-cell epitope from foot-and-mouth disease virus protein 3A improves humoral response against classical swine fever virusBohórquez, José AlejandroDefaus, SiraMuñoz González, SaraPerez-Simó, MartaRosell, RosaFraile Sauce, Lorenzo JoséSobrino, FranciscoAndreu, DavidGanges, LlilianneB cell epitopeCSFVDendrimeric peptideFMDVThree dendrimeric peptides were synthesized in order to evaluate their immunogenicity and their potential protection against classical swine fever virus (CSFV) in domestic pigs. Construct 1, an optimized version of a previously used dendrimer, had four copies of a B-cell epitope derived from CSFV E2 glycoprotein connected to an also CSFV-derived T-cell epitope through maleimide instead of thioether linkages. Construct 2 was similarly built but included only two copies of the B-cell epitope, and in also bivalent construct 3 the CSFV T-cell epitope was replaced by a previously described one from the 3A protein of foot-and-mouth disease virus (FMDV). Animals were inoculated twice with a 21-day interval and challenged 15days after the second immunization. Clinical signs were recorded daily and ELISA tests were performed to detect antibodies against specific peptide and E2. The neutralising antibody response was assessed 13days after challenge. Despite the change to maleimide connectivity, only partial protection against CSFV was again observed. The best clinical protection was observed in group 3. Animals inoculated with constructs 2 and 3 showed higher anti-peptide humoral response, suggesting that two copies of the B-cell epitope are sufficient or even better than four copies for swine immune recognition. In addition, for construct 3 higher neutralizing antibody titres against CSFV were detected. Our results support the immunogenicity of the CSFV B-cell epitope and the cooperative role of the FMDV 3A T-cell epitope in inducing a neutralising response against CSFV in domestic pigs. This is also the first time that the FMDV T-cell epitope shows effectivity in improving swine immune response against a different virus. Our findings highlight the relevance of dendrimeric peptides as a powerful tool for epitope characterization and antiviral strategies development.The research in CReSA was supported by grant AGL2015-66907 from the Spanish government. J.A. B. had a pre-doctoralfellowship FPI-MINECO 2016 from Spanish government. S. M.had a pre-doctoral fellowship FI-DGR 2014 from AGAUR, Gen-eralitat de Catalunya. Work at CBMSO was supported by grantsAGL2014-52395-C2-01 (MINECO, Spain) and S2013/ABI-2906-PLATESA (Comunidad Autónoma de Madrid). Work at UPF wasfunded by AGL2014-52395-C2-02 (MINECO, Spain).Elsevier2018201820172018info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://doi.org/10.1016/j.virusres.2017.05.020http://hdl.handle.net/10459.1/64676http://hdl.handle.net/10459.1/64676reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/grantAgreement/MINECO//AGL2015-66907-Rinfo:eu-repo/grantAgreement/MINECO//AGL2014-52395-C2-1-Rinfo:eu-repo/grantAgreement/MINECO//AGL2014-52395-C2-2-RVersió postprint del document publicat a: https://doi.org/10.1016/j.virusres.2017.05.020Virus Research, 2017, vol. 238, p. 8-12cc-by-nc-nd, (c) Elsevier, 2017info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:recercat.cat:10459.1/646762026-05-29T05:05:01Z
dc.title.none.fl_str_mv A bivalent dendrimeric peptide bearing a T-cell epitope from foot-and-mouth disease virus protein 3A improves humoral response against classical swine fever virus
title A bivalent dendrimeric peptide bearing a T-cell epitope from foot-and-mouth disease virus protein 3A improves humoral response against classical swine fever virus
spellingShingle A bivalent dendrimeric peptide bearing a T-cell epitope from foot-and-mouth disease virus protein 3A improves humoral response against classical swine fever virus
Bohórquez, José Alejandro
B cell epitope
CSFV
Dendrimeric peptide
FMDV
title_short A bivalent dendrimeric peptide bearing a T-cell epitope from foot-and-mouth disease virus protein 3A improves humoral response against classical swine fever virus
title_full A bivalent dendrimeric peptide bearing a T-cell epitope from foot-and-mouth disease virus protein 3A improves humoral response against classical swine fever virus
title_fullStr A bivalent dendrimeric peptide bearing a T-cell epitope from foot-and-mouth disease virus protein 3A improves humoral response against classical swine fever virus
title_full_unstemmed A bivalent dendrimeric peptide bearing a T-cell epitope from foot-and-mouth disease virus protein 3A improves humoral response against classical swine fever virus
title_sort A bivalent dendrimeric peptide bearing a T-cell epitope from foot-and-mouth disease virus protein 3A improves humoral response against classical swine fever virus
dc.creator.none.fl_str_mv Bohórquez, José Alejandro
Defaus, Sira
Muñoz González, Sara
Perez-Simó, Marta
Rosell, Rosa
Fraile Sauce, Lorenzo José
Sobrino, Francisco
Andreu, David
Ganges, Llilianne
author Bohórquez, José Alejandro
author_facet Bohórquez, José Alejandro
Defaus, Sira
Muñoz González, Sara
Perez-Simó, Marta
Rosell, Rosa
Fraile Sauce, Lorenzo José
Sobrino, Francisco
Andreu, David
Ganges, Llilianne
author_role author
author2 Defaus, Sira
Muñoz González, Sara
Perez-Simó, Marta
Rosell, Rosa
Fraile Sauce, Lorenzo José
Sobrino, Francisco
Andreu, David
Ganges, Llilianne
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv B cell epitope
CSFV
Dendrimeric peptide
FMDV
topic B cell epitope
CSFV
Dendrimeric peptide
FMDV
description Three dendrimeric peptides were synthesized in order to evaluate their immunogenicity and their potential protection against classical swine fever virus (CSFV) in domestic pigs. Construct 1, an optimized version of a previously used dendrimer, had four copies of a B-cell epitope derived from CSFV E2 glycoprotein connected to an also CSFV-derived T-cell epitope through maleimide instead of thioether linkages. Construct 2 was similarly built but included only two copies of the B-cell epitope, and in also bivalent construct 3 the CSFV T-cell epitope was replaced by a previously described one from the 3A protein of foot-and-mouth disease virus (FMDV). Animals were inoculated twice with a 21-day interval and challenged 15days after the second immunization. Clinical signs were recorded daily and ELISA tests were performed to detect antibodies against specific peptide and E2. The neutralising antibody response was assessed 13days after challenge. Despite the change to maleimide connectivity, only partial protection against CSFV was again observed. The best clinical protection was observed in group 3. Animals inoculated with constructs 2 and 3 showed higher anti-peptide humoral response, suggesting that two copies of the B-cell epitope are sufficient or even better than four copies for swine immune recognition. In addition, for construct 3 higher neutralizing antibody titres against CSFV were detected. Our results support the immunogenicity of the CSFV B-cell epitope and the cooperative role of the FMDV 3A T-cell epitope in inducing a neutralising response against CSFV in domestic pigs. This is also the first time that the FMDV T-cell epitope shows effectivity in improving swine immune response against a different virus. Our findings highlight the relevance of dendrimeric peptides as a powerful tool for epitope characterization and antiviral strategies development.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.virusres.2017.05.020
http://hdl.handle.net/10459.1/64676
http://hdl.handle.net/10459.1/64676
url https://doi.org/10.1016/j.virusres.2017.05.020
http://hdl.handle.net/10459.1/64676
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO//AGL2015-66907-R
info:eu-repo/grantAgreement/MINECO//AGL2014-52395-C2-1-R
info:eu-repo/grantAgreement/MINECO//AGL2014-52395-C2-2-R
Versió postprint del document publicat a: https://doi.org/10.1016/j.virusres.2017.05.020
Virus Research, 2017, vol. 238, p. 8-12
dc.rights.none.fl_str_mv cc-by-nc-nd, (c) Elsevier, 2017
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
rights_invalid_str_mv cc-by-nc-nd, (c) Elsevier, 2017
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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