Excitation energy transfer in chlorosomes of Chlorobium phaeobacteroides strain CL1401: the role of carotenoids

The role of carotenoids in chlorosomes of the green sulfur bacterium Chlorobium phaeobacteroides, containing bacteriochlorophyll (BChl) e and the carotenoid (Car) isorenieratene as main pigments, was studied by steady-state fluorescence excitation, picosecond single-photon timing and femtosecond tra...

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Autores: Psencik, Jakub, Ma, Ying-Zhong, Arellano, Juan B., García-Gil, L. Jesús, Holzwarth, Alfred R., Gillbro, Tomas
Formato: artículo
Fecha de publicación:2002
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::f6e1ea09d474bf39dddc30bb1cec5357
Acesso em linha:http://hdl.handle.net/10261/7618
Access Level:acceso abierto
Palavra-chave:Bacteriochlorophyll
Carotenoids
Chlorosomes
Chlorobiaceae
Energy transfer
femtosecond spectroscopy
fluorescence excitation
green sulfur bacteria
single-photon timing
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spelling Excitation energy transfer in chlorosomes of Chlorobium phaeobacteroides strain CL1401: the role of carotenoidsPsencik, JakubMa, Ying-ZhongArellano, Juan B.García-Gil, L. JesúsHolzwarth, Alfred R.Gillbro, TomasBacteriochlorophyllCarotenoidsChlorosomesChlorobiaceaeEnergy transferfemtosecond spectroscopyfluorescence excitationgreen sulfur bacteriasingle-photon timingThe role of carotenoids in chlorosomes of the green sulfur bacterium Chlorobium phaeobacteroides, containing bacteriochlorophyll (BChl) e and the carotenoid (Car) isorenieratene as main pigments, was studied by steady-state fluorescence excitation, picosecond single-photon timing and femtosecond transient absorption (TA) spectroscopy. In order to obtain information about energy transfer from Cars in this photosynthetic light-harvesting antenna with high spectral overlap between Cars and BChls, Car-depleted chlorosomes, obtained by inhibition of Car biosynthesis by 2-hydroxybiphenyl, were employed in a comparative study with control chlorosomes. Excitation spectra measured at room temperature give an efficiency of 60-70% for the excitation energy transfer from Cars to BChls in control chlorosomes. Femtosecond TA measurements enabled an identification of the excited state absorption band of Cars and the lifetime of their S-1 state was determined to be similar to10 ps. Based on this lifetime, we concluded that the involvement of this state in energy transfer is unlikely. Furthermore, evidence was obtained for the presence of an ultrafast (<100 fs) energy transfer process from the S-2 state of Cars to BChls in control chlorosomes. Using two time-resolved techniques, we further found that the absence of Cars leads to overall slower decay kinetics probed within the Qy band of BChl e aggregates, and that two time constants are generally required to describe energy transfer from aggregated BChl e to baseplate BChl a.Swedish Natural Science Research Council. European Union (Contract FMRX-CT96-0081). Kempe FoundationPeer reviewedSpringer Nature200820082002info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501388815 bytesapplication/pdfhttp://hdl.handle.net/10261/7618reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://www.springerlink.com/content/nwvtdvnlu1n0atpv/?p=b2b8dcf01d9242358e430be8eb155c85&pi=1info:eu-repo/semantics/openAccessoai:dnet:digitalcsic_::f6e1ea09d474bf39dddc30bb1cec53572026-05-22T06:33:51Z
dc.title.none.fl_str_mv Excitation energy transfer in chlorosomes of Chlorobium phaeobacteroides strain CL1401: the role of carotenoids
title Excitation energy transfer in chlorosomes of Chlorobium phaeobacteroides strain CL1401: the role of carotenoids
spellingShingle Excitation energy transfer in chlorosomes of Chlorobium phaeobacteroides strain CL1401: the role of carotenoids
Psencik, Jakub
Bacteriochlorophyll
Carotenoids
Chlorosomes
Chlorobiaceae
Energy transfer
femtosecond spectroscopy
fluorescence excitation
green sulfur bacteria
single-photon timing
title_short Excitation energy transfer in chlorosomes of Chlorobium phaeobacteroides strain CL1401: the role of carotenoids
title_full Excitation energy transfer in chlorosomes of Chlorobium phaeobacteroides strain CL1401: the role of carotenoids
title_fullStr Excitation energy transfer in chlorosomes of Chlorobium phaeobacteroides strain CL1401: the role of carotenoids
title_full_unstemmed Excitation energy transfer in chlorosomes of Chlorobium phaeobacteroides strain CL1401: the role of carotenoids
title_sort Excitation energy transfer in chlorosomes of Chlorobium phaeobacteroides strain CL1401: the role of carotenoids
dc.creator.none.fl_str_mv Psencik, Jakub
Ma, Ying-Zhong
Arellano, Juan B.
García-Gil, L. Jesús
Holzwarth, Alfred R.
Gillbro, Tomas
author Psencik, Jakub
author_facet Psencik, Jakub
Ma, Ying-Zhong
Arellano, Juan B.
García-Gil, L. Jesús
Holzwarth, Alfred R.
Gillbro, Tomas
author_role author
author2 Ma, Ying-Zhong
Arellano, Juan B.
García-Gil, L. Jesús
Holzwarth, Alfred R.
Gillbro, Tomas
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bacteriochlorophyll
Carotenoids
Chlorosomes
Chlorobiaceae
Energy transfer
femtosecond spectroscopy
fluorescence excitation
green sulfur bacteria
single-photon timing
topic Bacteriochlorophyll
Carotenoids
Chlorosomes
Chlorobiaceae
Energy transfer
femtosecond spectroscopy
fluorescence excitation
green sulfur bacteria
single-photon timing
description The role of carotenoids in chlorosomes of the green sulfur bacterium Chlorobium phaeobacteroides, containing bacteriochlorophyll (BChl) e and the carotenoid (Car) isorenieratene as main pigments, was studied by steady-state fluorescence excitation, picosecond single-photon timing and femtosecond transient absorption (TA) spectroscopy. In order to obtain information about energy transfer from Cars in this photosynthetic light-harvesting antenna with high spectral overlap between Cars and BChls, Car-depleted chlorosomes, obtained by inhibition of Car biosynthesis by 2-hydroxybiphenyl, were employed in a comparative study with control chlorosomes. Excitation spectra measured at room temperature give an efficiency of 60-70% for the excitation energy transfer from Cars to BChls in control chlorosomes. Femtosecond TA measurements enabled an identification of the excited state absorption band of Cars and the lifetime of their S-1 state was determined to be similar to10 ps. Based on this lifetime, we concluded that the involvement of this state in energy transfer is unlikely. Furthermore, evidence was obtained for the presence of an ultrafast (<100 fs) energy transfer process from the S-2 state of Cars to BChls in control chlorosomes. Using two time-resolved techniques, we further found that the absence of Cars leads to overall slower decay kinetics probed within the Qy band of BChl e aggregates, and that two time constants are generally required to describe energy transfer from aggregated BChl e to baseplate BChl a.
publishDate 2002
dc.date.none.fl_str_mv 2002
2008
2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/7618
url http://hdl.handle.net/10261/7618
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://www.springerlink.com/content/nwvtdvnlu1n0atpv/?p=b2b8dcf01d9242358e430be8eb155c85&pi=1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 388815 bytes
application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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