Functional characterisation of two Δ12-desaturases demonstrates targeted production of linoleic acid as pheromone precursor in Nasonia

Insect pheromones are often derived from fatty acid metabolism. Fatty acid desaturases, enzymes introducing double bonds into fatty acids, are crucial for the biosynthesis of these chemical signals. Δ12-desaturases catalyse the biosynthesis of linoleic acid by introducing a second double bond into o...

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Autores: Semmelmann, Florian, Kabeya, Naoki, Malcicka, Miriama, Bruckmann, Astrid, Broschwitz, Batian, Straub, Kristina, Merkl, Rainer, Monroig, Óscar, Sterner, Reinhard, Ruther, Joachim, Ellers, J.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/202099
Acceso en línea:http://hdl.handle.net/10261/202099
Access Level:acceso abierto
Palabra clave:Parasitic wasp
Δ12-desaturase
Sex pheromone
Biosynthesis
Nasonia vitripennis
Urolepis rufipes
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spelling Functional characterisation of two Δ12-desaturases demonstrates targeted production of linoleic acid as pheromone precursor in NasoniaSemmelmann, FlorianKabeya, NaokiMalcicka, MiriamaBruckmann, AstridBroschwitz, BatianStraub, KristinaMerkl, RainerMonroig, ÓscarSterner, ReinhardRuther, JoachimEllers, J.Parasitic waspΔ12-desaturaseSex pheromoneBiosynthesisNasonia vitripennisUrolepis rufipesInsect pheromones are often derived from fatty acid metabolism. Fatty acid desaturases, enzymes introducing double bonds into fatty acids, are crucial for the biosynthesis of these chemical signals. Δ12-desaturases catalyse the biosynthesis of linoleic acid by introducing a second double bond into oleic acid, but have been identified in only a few animal species. Here, we report the functional characterisation of two Δ12-desaturases, Nvit_D12a and Nvit_D12b, from the parasitic wasp Nasonia vitripennis. We demonstrate that Nvit_D12a is expressed in the rectal vesicle of males where they produce a linoleic acid-derived sex pheromone to attract virgin females. C-labelling experiments with Urolepis rufipes, a closely related species belonging to the ‘Nasonia group’, revealed that females, but not males, are able to synthesise linoleic acid. U. rufipes males produce an isoprenoid sex pheromone in the same gland and do not depend on linoleic acid for pheromone production. This suggests that Δ12-desaturases are common in the ‘Nasonia group’, but acquired a specialised function in chemical communication of those species that use linoleic acid as a pheromone precursor. Phylogenetic analysis suggests that insect Δ12-desaturases have evolved repeatedly from Δ9-desaturases in different insect taxa. Hence, insects have developed a way to produce linoleic acid independent of the omega desaturase subfamily which harbours all of the eukaryotic Δ12-desaturases known so far.F.S. was supported by a doctoral fellowship from the Konrad-Adenauer-Stiftung; J.E. and M.M. were supported by the Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NOW; VICI grant 865.12.003).Company of BiologistsKonrad Adenauer StiftungResearch Foundation - FlandersKabeya, Naoki [0000-0002-2055-6554]Malcicka, Miriama [0000-0001-9896-5000]Monroig, Óscar [0000-0001-8712-0440]Ruther, Joachim [0000-0002-1295-347X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020192020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/202099reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1242/jeb.201038Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2020992026-05-22T06:33:51Z
dc.title.none.fl_str_mv Functional characterisation of two Δ12-desaturases demonstrates targeted production of linoleic acid as pheromone precursor in Nasonia
title Functional characterisation of two Δ12-desaturases demonstrates targeted production of linoleic acid as pheromone precursor in Nasonia
spellingShingle Functional characterisation of two Δ12-desaturases demonstrates targeted production of linoleic acid as pheromone precursor in Nasonia
Semmelmann, Florian
Parasitic wasp
Δ12-desaturase
Sex pheromone
Biosynthesis
Nasonia vitripennis
Urolepis rufipes
title_short Functional characterisation of two Δ12-desaturases demonstrates targeted production of linoleic acid as pheromone precursor in Nasonia
title_full Functional characterisation of two Δ12-desaturases demonstrates targeted production of linoleic acid as pheromone precursor in Nasonia
title_fullStr Functional characterisation of two Δ12-desaturases demonstrates targeted production of linoleic acid as pheromone precursor in Nasonia
title_full_unstemmed Functional characterisation of two Δ12-desaturases demonstrates targeted production of linoleic acid as pheromone precursor in Nasonia
title_sort Functional characterisation of two Δ12-desaturases demonstrates targeted production of linoleic acid as pheromone precursor in Nasonia
dc.creator.none.fl_str_mv Semmelmann, Florian
Kabeya, Naoki
Malcicka, Miriama
Bruckmann, Astrid
Broschwitz, Batian
Straub, Kristina
Merkl, Rainer
Monroig, Óscar
Sterner, Reinhard
Ruther, Joachim
Ellers, J.
author Semmelmann, Florian
author_facet Semmelmann, Florian
Kabeya, Naoki
Malcicka, Miriama
Bruckmann, Astrid
Broschwitz, Batian
Straub, Kristina
Merkl, Rainer
Monroig, Óscar
Sterner, Reinhard
Ruther, Joachim
Ellers, J.
author_role author
author2 Kabeya, Naoki
Malcicka, Miriama
Bruckmann, Astrid
Broschwitz, Batian
Straub, Kristina
Merkl, Rainer
Monroig, Óscar
Sterner, Reinhard
Ruther, Joachim
Ellers, J.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Konrad Adenauer Stiftung
Research Foundation - Flanders
Kabeya, Naoki [0000-0002-2055-6554]
Malcicka, Miriama [0000-0001-9896-5000]
Monroig, Óscar [0000-0001-8712-0440]
Ruther, Joachim [0000-0002-1295-347X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Parasitic wasp
Δ12-desaturase
Sex pheromone
Biosynthesis
Nasonia vitripennis
Urolepis rufipes
topic Parasitic wasp
Δ12-desaturase
Sex pheromone
Biosynthesis
Nasonia vitripennis
Urolepis rufipes
description Insect pheromones are often derived from fatty acid metabolism. Fatty acid desaturases, enzymes introducing double bonds into fatty acids, are crucial for the biosynthesis of these chemical signals. Δ12-desaturases catalyse the biosynthesis of linoleic acid by introducing a second double bond into oleic acid, but have been identified in only a few animal species. Here, we report the functional characterisation of two Δ12-desaturases, Nvit_D12a and Nvit_D12b, from the parasitic wasp Nasonia vitripennis. We demonstrate that Nvit_D12a is expressed in the rectal vesicle of males where they produce a linoleic acid-derived sex pheromone to attract virgin females. C-labelling experiments with Urolepis rufipes, a closely related species belonging to the ‘Nasonia group’, revealed that females, but not males, are able to synthesise linoleic acid. U. rufipes males produce an isoprenoid sex pheromone in the same gland and do not depend on linoleic acid for pheromone production. This suggests that Δ12-desaturases are common in the ‘Nasonia group’, but acquired a specialised function in chemical communication of those species that use linoleic acid as a pheromone precursor. Phylogenetic analysis suggests that insect Δ12-desaturases have evolved repeatedly from Δ9-desaturases in different insect taxa. Hence, insects have developed a way to produce linoleic acid independent of the omega desaturase subfamily which harbours all of the eukaryotic Δ12-desaturases known so far.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/202099
url http://hdl.handle.net/10261/202099
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1242/jeb.201038
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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