Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4

The epicardium plays a crucial role in embryonic heart development and adult heart repair; however, the molecular events underlying its maturation remain unknown. Wt1, one of the main markers of the embryonic epicardium, is essential for epicardial development and function. Here, we analyse the tran...

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Detalles Bibliográficos
Autores: Velecela, Victor, Torres Cano, Alejo, García Melero, Ana, Ramiro-Pareta, Marina, Müller Sánchez, Claudia Alejandra, Segarra Mondéjar, Marc, Chau, You-Ying, Campos Bonilla, Begoña, Reina del Pozo, Manuel, Soriano Zaragoza, Francesc X. (Francesc Xavier), Hastie, Nicholas D., Martinez, Fernando O., Martínez Estrada, Ofelia María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/175561
Acceso en línea:https://hdl.handle.net/2445/175561
Access Level:acceso abierto
Palabra clave:Cèl·lules epitelials
Epithelial cells
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spelling Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4Velecela, VictorTorres Cano, AlejoGarcía Melero, AnaRamiro-Pareta, MarinaMüller Sánchez, Claudia AlejandraSegarra Mondéjar, MarcChau, You-YingCampos Bonilla, BegoñaReina del Pozo, ManuelSoriano Zaragoza, Francesc X. (Francesc Xavier)Hastie, Nicholas D.Martinez, Fernando O.Martínez Estrada, Ofelia MaríaCèl·lules epitelialsEpithelial cellsThe epicardium plays a crucial role in embryonic heart development and adult heart repair; however, the molecular events underlying its maturation remain unknown. Wt1, one of the main markers of the embryonic epicardium, is essential for epicardial development and function. Here, we analyse the transcriptomic profile of epicardial-enriched cells at different stages of development and from control and epicardial-specific Wt1 knockout (Wt1KO) mice. Transcriptomic and cell morphology analyses of epicardial cells from epicardial-specific Wt1KO mice revealed a defect in the maturation process of the mutant epicardium, including sustained upregulation of Bmp4 expression and the inability of mutant epicardial cells to transition into a mature squamous phenotype. We identified Bmp4 as a transcriptional target of Wt1, thus providing a molecular basis for the retention of the cuboidal cell shape observed in the Wt1KO epicardium. Accordingly, inhibition of the Bmp4 signalling pathway both ex vivo and in vivo rescued the cuboidal phenotype of the mutant epicardium. Our findings indicate the importance of the cuboidal-to-squamous transition in epicardial maturation, a process regulated by Wt1.The Company of Biologists2021202120192021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion14 p.application/pdfhttps://hdl.handle.net/2445/175561Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1242/dev.178723Development, 2019, vol. 146, num. 20https://doi.org/10.1242/dev.178723(c) Velecela, Victor et al., 2019info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1755612026-05-29T05:05:01Z
dc.title.none.fl_str_mv Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4
title Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4
spellingShingle Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4
Velecela, Victor
Cèl·lules epitelials
Epithelial cells
title_short Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4
title_full Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4
title_fullStr Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4
title_full_unstemmed Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4
title_sort Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4
dc.creator.none.fl_str_mv Velecela, Victor
Torres Cano, Alejo
García Melero, Ana
Ramiro-Pareta, Marina
Müller Sánchez, Claudia Alejandra
Segarra Mondéjar, Marc
Chau, You-Ying
Campos Bonilla, Begoña
Reina del Pozo, Manuel
Soriano Zaragoza, Francesc X. (Francesc Xavier)
Hastie, Nicholas D.
Martinez, Fernando O.
Martínez Estrada, Ofelia María
author Velecela, Victor
author_facet Velecela, Victor
Torres Cano, Alejo
García Melero, Ana
Ramiro-Pareta, Marina
Müller Sánchez, Claudia Alejandra
Segarra Mondéjar, Marc
Chau, You-Ying
Campos Bonilla, Begoña
Reina del Pozo, Manuel
Soriano Zaragoza, Francesc X. (Francesc Xavier)
Hastie, Nicholas D.
Martinez, Fernando O.
Martínez Estrada, Ofelia María
author_role author
author2 Torres Cano, Alejo
García Melero, Ana
Ramiro-Pareta, Marina
Müller Sánchez, Claudia Alejandra
Segarra Mondéjar, Marc
Chau, You-Ying
Campos Bonilla, Begoña
Reina del Pozo, Manuel
Soriano Zaragoza, Francesc X. (Francesc Xavier)
Hastie, Nicholas D.
Martinez, Fernando O.
Martínez Estrada, Ofelia María
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cèl·lules epitelials
Epithelial cells
topic Cèl·lules epitelials
Epithelial cells
description The epicardium plays a crucial role in embryonic heart development and adult heart repair; however, the molecular events underlying its maturation remain unknown. Wt1, one of the main markers of the embryonic epicardium, is essential for epicardial development and function. Here, we analyse the transcriptomic profile of epicardial-enriched cells at different stages of development and from control and epicardial-specific Wt1 knockout (Wt1KO) mice. Transcriptomic and cell morphology analyses of epicardial cells from epicardial-specific Wt1KO mice revealed a defect in the maturation process of the mutant epicardium, including sustained upregulation of Bmp4 expression and the inability of mutant epicardial cells to transition into a mature squamous phenotype. We identified Bmp4 as a transcriptional target of Wt1, thus providing a molecular basis for the retention of the cuboidal cell shape observed in the Wt1KO epicardium. Accordingly, inhibition of the Bmp4 signalling pathway both ex vivo and in vivo rescued the cuboidal phenotype of the mutant epicardium. Our findings indicate the importance of the cuboidal-to-squamous transition in epicardial maturation, a process regulated by Wt1.
publishDate 2019
dc.date.none.fl_str_mv 2019
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/175561
url https://hdl.handle.net/2445/175561
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1242/dev.178723
Development, 2019, vol. 146, num. 20
https://doi.org/10.1242/dev.178723
dc.rights.none.fl_str_mv (c) Velecela, Victor et al., 2019
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Velecela, Victor et al., 2019
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 14 p.
application/pdf
dc.publisher.none.fl_str_mv The Company of Biologists
publisher.none.fl_str_mv The Company of Biologists
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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