Anti-TIF-1γ Antibody Detection Using a Commercial Kit vs In-House Immunoblot
Anti-TIF-1γ autoantibody detection is important for cancer screening in patients with dermatomyositis. The gold standard for anti-TIF-1γ detection, immunoprecipitation, is only available from a few specialized laboratories worldwide, so commercial ELISA/immunoblot tests have emerged in recent years....
| Autores: | , , , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:255307 |
| Acceso en línea: | https://ddd.uab.cat/record/255307 https://dx.doi.org/urn:doi:10.3389/fimmu.2020.625896 |
| Access Level: | acceso abierto |
| Palabra clave: | Dermatomyositis Cancer Autoantibody Diagnosis Immunoassay |
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Anti-TIF-1γ Antibody Detection Using a Commercial Kit vs In-House ImmunoblotUsefulness in Clinical PracticeMariscal, Anaís|||0000-0003-1013-4793Milán, MilenaBaucells, Andrés|||0000-0002-4567-8486Martínez, Maria Angeles|||0000-0002-2192-8719Garcia Guillen, AndreaTrallero Araguás, Ernesto|||0000-0003-3365-4521Alvarado, Marcelo|||0000-0003-2915-8963Martinez-Martinez, Laura|||0000-0003-0126-158XAlserawan, LeticiaFranco Leyva, Teresa|||0000-0002-0250-0274Sanz-Martínez, María Teresa|||0000-0001-5814-8698Viñas-Giménez, Laura|||0000-0003-2913-1154Corominas, Hèctor|||0000-0002-7738-6787Juárez Rubio, Cándido|||0000-0003-2235-9893Castellvi, Ivan|||0000-0002-5410-5807Selva O'Callaghan, Albert|||0000-0003-2823-9761DermatomyositisCancerAutoantibodyDiagnosisImmunoassayAnti-TIF-1γ autoantibody detection is important for cancer screening in patients with dermatomyositis. The gold standard for anti-TIF-1γ detection, immunoprecipitation, is only available from a few specialized laboratories worldwide, so commercial ELISA/immunoblot tests have emerged in recent years. To analyze their usefulness in diagnosing cancer-associated dermatomyositis, we compared Euroimmun Euroline profile with our previously validated in-house immunoblot assay with human recombinant TIF-1γ. We included 308 adult patients from Hospital de la Santa Creu I Sant Pau and Vall Hebrón Hospital (Barcelona, Spain) tested for anti-TIF-1γ autoantibodies using the Euroline profile and an in-house immunoblot assay. A total of 27 anti-TIF-1γ were detected by the Euroline and 12 by the in-house assay. Fair agreement was observed between Euroline and the in-house immunoblot Cohen's kappa 0.3163. Expected prevalence of anti-TIF-1γ autoantibodies was observed for the two methods for dermatomyositis and undifferentiated connective tissue diseases, but unexpectedly high prevalence of anti-TIF-1γ autoantibodies was detected by Euroline compared to the in-house immunoblot for other diseases (16.5% Euroline vs 0.8% in-house immunoblot, p<0.01). The in-house IB compared to Euroline more reliably detected cancer in patients with DM with anti-TIF-1γ antibodies (p=0.0014 vs p=0.0502 for in-house immunoblot vs Euroline). We recommend using a second validated method to confirm Euroline-detected anti-TIF-1γ antibodies when the dermatomyositis diagnosis is not definitive. Furthermore, in the context of definite DM diagnosis with negative anti-TIF-1γ antibodies by Euroline and no other myositis specific antibody, is also recommendable to confirm by a second validated method.Universitat Autònoma de Barcelona 22021-01-0120212021-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/255307https://dx.doi.org/urn:doi:10.3389/fimmu.2020.625896reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2553072026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Anti-TIF-1γ Antibody Detection Using a Commercial Kit vs In-House Immunoblot Usefulness in Clinical Practice |
| title |
Anti-TIF-1γ Antibody Detection Using a Commercial Kit vs In-House Immunoblot |
| spellingShingle |
Anti-TIF-1γ Antibody Detection Using a Commercial Kit vs In-House Immunoblot Mariscal, Anaís|||0000-0003-1013-4793 Dermatomyositis Cancer Autoantibody Diagnosis Immunoassay |
| title_short |
Anti-TIF-1γ Antibody Detection Using a Commercial Kit vs In-House Immunoblot |
| title_full |
Anti-TIF-1γ Antibody Detection Using a Commercial Kit vs In-House Immunoblot |
| title_fullStr |
Anti-TIF-1γ Antibody Detection Using a Commercial Kit vs In-House Immunoblot |
| title_full_unstemmed |
Anti-TIF-1γ Antibody Detection Using a Commercial Kit vs In-House Immunoblot |
| title_sort |
Anti-TIF-1γ Antibody Detection Using a Commercial Kit vs In-House Immunoblot |
| dc.creator.none.fl_str_mv |
Mariscal, Anaís|||0000-0003-1013-4793 Milán, Milena Baucells, Andrés|||0000-0002-4567-8486 Martínez, Maria Angeles|||0000-0002-2192-8719 Garcia Guillen, Andrea Trallero Araguás, Ernesto|||0000-0003-3365-4521 Alvarado, Marcelo|||0000-0003-2915-8963 Martinez-Martinez, Laura|||0000-0003-0126-158X Alserawan, Leticia Franco Leyva, Teresa|||0000-0002-0250-0274 Sanz-Martínez, María Teresa|||0000-0001-5814-8698 Viñas-Giménez, Laura|||0000-0003-2913-1154 Corominas, Hèctor|||0000-0002-7738-6787 Juárez Rubio, Cándido|||0000-0003-2235-9893 Castellvi, Ivan|||0000-0002-5410-5807 Selva O'Callaghan, Albert|||0000-0003-2823-9761 |
| author |
Mariscal, Anaís|||0000-0003-1013-4793 |
| author_facet |
Mariscal, Anaís|||0000-0003-1013-4793 Milán, Milena Baucells, Andrés|||0000-0002-4567-8486 Martínez, Maria Angeles|||0000-0002-2192-8719 Garcia Guillen, Andrea Trallero Araguás, Ernesto|||0000-0003-3365-4521 Alvarado, Marcelo|||0000-0003-2915-8963 Martinez-Martinez, Laura|||0000-0003-0126-158X Alserawan, Leticia Franco Leyva, Teresa|||0000-0002-0250-0274 Sanz-Martínez, María Teresa|||0000-0001-5814-8698 Viñas-Giménez, Laura|||0000-0003-2913-1154 Corominas, Hèctor|||0000-0002-7738-6787 Juárez Rubio, Cándido|||0000-0003-2235-9893 Castellvi, Ivan|||0000-0002-5410-5807 Selva O'Callaghan, Albert|||0000-0003-2823-9761 |
| author_role |
author |
| author2 |
Milán, Milena Baucells, Andrés|||0000-0002-4567-8486 Martínez, Maria Angeles|||0000-0002-2192-8719 Garcia Guillen, Andrea Trallero Araguás, Ernesto|||0000-0003-3365-4521 Alvarado, Marcelo|||0000-0003-2915-8963 Martinez-Martinez, Laura|||0000-0003-0126-158X Alserawan, Leticia Franco Leyva, Teresa|||0000-0002-0250-0274 Sanz-Martínez, María Teresa|||0000-0001-5814-8698 Viñas-Giménez, Laura|||0000-0003-2913-1154 Corominas, Hèctor|||0000-0002-7738-6787 Juárez Rubio, Cándido|||0000-0003-2235-9893 Castellvi, Ivan|||0000-0002-5410-5807 Selva O'Callaghan, Albert|||0000-0003-2823-9761 |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universitat Autònoma de Barcelona |
| dc.subject.none.fl_str_mv |
Dermatomyositis Cancer Autoantibody Diagnosis Immunoassay |
| topic |
Dermatomyositis Cancer Autoantibody Diagnosis Immunoassay |
| description |
Anti-TIF-1γ autoantibody detection is important for cancer screening in patients with dermatomyositis. The gold standard for anti-TIF-1γ detection, immunoprecipitation, is only available from a few specialized laboratories worldwide, so commercial ELISA/immunoblot tests have emerged in recent years. To analyze their usefulness in diagnosing cancer-associated dermatomyositis, we compared Euroimmun Euroline profile with our previously validated in-house immunoblot assay with human recombinant TIF-1γ. We included 308 adult patients from Hospital de la Santa Creu I Sant Pau and Vall Hebrón Hospital (Barcelona, Spain) tested for anti-TIF-1γ autoantibodies using the Euroline profile and an in-house immunoblot assay. A total of 27 anti-TIF-1γ were detected by the Euroline and 12 by the in-house assay. Fair agreement was observed between Euroline and the in-house immunoblot Cohen's kappa 0.3163. Expected prevalence of anti-TIF-1γ autoantibodies was observed for the two methods for dermatomyositis and undifferentiated connective tissue diseases, but unexpectedly high prevalence of anti-TIF-1γ autoantibodies was detected by Euroline compared to the in-house immunoblot for other diseases (16.5% Euroline vs 0.8% in-house immunoblot, p<0.01). The in-house IB compared to Euroline more reliably detected cancer in patients with DM with anti-TIF-1γ antibodies (p=0.0014 vs p=0.0502 for in-house immunoblot vs Euroline). We recommend using a second validated method to confirm Euroline-detected anti-TIF-1γ antibodies when the dermatomyositis diagnosis is not definitive. Furthermore, in the context of definite DM diagnosis with negative anti-TIF-1γ antibodies by Euroline and no other myositis specific antibody, is also recommendable to confirm by a second validated method. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2 2021-01-01 2021 2021-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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article |
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https://ddd.uab.cat/record/255307 https://dx.doi.org/urn:doi:10.3389/fimmu.2020.625896 |
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https://ddd.uab.cat/record/255307 https://dx.doi.org/urn:doi:10.3389/fimmu.2020.625896 |
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Inglés eng |
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Inglés |
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eng |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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