Consumer electronics devices for DNA genotyping based on loop-mediated isothermal amplification and array hybridisation

[EN] Consumer electronic technologies offer practical performances to develop compact biosensing systems intended for the point-of-care testing of DNA biomarkers. Herein a discrimination method for detecting single nucleotide polymorphisms, based on isothermal amplification and on-chip hybridisation...

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Detalles Bibliográficos
Autores: Tortajada-Genaro, Luis Antonio|||0000-0003-4021-5607, Maquieira, Angel|||0000-0003-4641-4957, Yamanaka, Eric Seiti
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/141451
Acceso en línea:https://riunet.upv.es/handle/10251/141451
Access Level:acceso abierto
Palabra clave:Single-nucleotide polymorphism
Isothermal DNA amplification
Point-of-care testing
Smartphone
Scanner
Compact disc
QUIMICA ANALITICA
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spelling Consumer electronics devices for DNA genotyping based on loop-mediated isothermal amplification and array hybridisationTortajada-Genaro, Luis Antonio|||0000-0003-4021-5607Maquieira, Angel|||0000-0003-4641-4957Yamanaka, Eric SeitiSingle-nucleotide polymorphismIsothermal DNA amplificationPoint-of-care testingSmartphoneScannerCompact discQUIMICA ANALITICA[EN] Consumer electronic technologies offer practical performances to develop compact biosensing systems intended for the point-of-care testing of DNA biomarkers. Herein a discrimination method for detecting single nucleotide polymorphisms, based on isothermal amplification and on-chip hybridisation, was developed and integrated into user-friendly optical devices: e.g., USB digital microscope, flatbed scanner, smartphone and DVD drive. In order to adequately identify a single base change, loop-mediated isothermal amplification (LAMP) was employed, with high yields (8 orders) within 45 min. Subsequently, products were directly hybridised to the allele-specific probes attached to plastic chips in an array format. After colorimetric staining, four consumer electronic techniques were compared. Sensitive precise measurements were taken (high signal-to-noise ratios, 10-mu m image resolution, 99% scan-to-scan reproducibility). These features confirmed their potential as analytical tools, are a competitive alternative to fluorescence scanners, and incorporate additional advantages, such as user-friendly interface and connectivity for telemedicine needs. The analytical performances of the integrated platform (assay and reader) in the human samples were also excellent, with a low detection limit (100 genomic DNA copies), and reproducible (< 15%) and cheap assays (< 10 (sic)/test). The correct genotyping of a genetic biomarker (single-nucleotide polymorphism located in the GRIK4 gene) was achieved as the assigned genotypes agreed with those determined by using sequencing. The portability, favourable discriminating and read-out capabilities reveal that the implementation of mass-produced low-cost devices into minimal-specialised clinical laboratories is closer to becoming a reality.The authors acknowledge the financial support received from the Generalitat Valenciana, Spain (GVA-PROMETEOII/2014/040 Project and GRISOLIA/2014/024 Ph.D. grant) and the Spanish Ministry of Economy and Competitiveness, Spain (MINECO CTQ2016-75749-R project) by U. E. FEDER funds. The authors also thank J. Carrascosa for supporting the DVD reader measurements.ElsevierDepartamento de QuímicaInstituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo TecnológicoEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalGeneralitat ValencianaMinisterio de Economía y CompetitividadRepositorio Institucional de la Universitat Politècnica de València Riunet20192019-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/141451reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengGeneralitat Valenciana https://doi.org/10.13039/501100003359 GRISOLIA%2F2014%2F024Generalitat Valenciana https://doi.org/10.13039/501100003359 PROMETEOII%2F2014%2F040 Estudio de estrategias fisico-químicas para el desarrollo de biosensores interferométricos en soportes interactivos de aplicación en clínicaMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 CTQ2016-75749-R BIOSENSORES HOLOGRAFICOS. PRUEBA DE CONCEPTO Y DEMOSTRACION EN APLICACIONES CLINICASopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1414512026-06-13T07:49:27Z
dc.title.none.fl_str_mv Consumer electronics devices for DNA genotyping based on loop-mediated isothermal amplification and array hybridisation
title Consumer electronics devices for DNA genotyping based on loop-mediated isothermal amplification and array hybridisation
spellingShingle Consumer electronics devices for DNA genotyping based on loop-mediated isothermal amplification and array hybridisation
Tortajada-Genaro, Luis Antonio|||0000-0003-4021-5607
Single-nucleotide polymorphism
Isothermal DNA amplification
Point-of-care testing
Smartphone
Scanner
Compact disc
QUIMICA ANALITICA
title_short Consumer electronics devices for DNA genotyping based on loop-mediated isothermal amplification and array hybridisation
title_full Consumer electronics devices for DNA genotyping based on loop-mediated isothermal amplification and array hybridisation
title_fullStr Consumer electronics devices for DNA genotyping based on loop-mediated isothermal amplification and array hybridisation
title_full_unstemmed Consumer electronics devices for DNA genotyping based on loop-mediated isothermal amplification and array hybridisation
title_sort Consumer electronics devices for DNA genotyping based on loop-mediated isothermal amplification and array hybridisation
dc.creator.none.fl_str_mv Tortajada-Genaro, Luis Antonio|||0000-0003-4021-5607
Maquieira, Angel|||0000-0003-4641-4957
Yamanaka, Eric Seiti
author Tortajada-Genaro, Luis Antonio|||0000-0003-4021-5607
author_facet Tortajada-Genaro, Luis Antonio|||0000-0003-4021-5607
Maquieira, Angel|||0000-0003-4641-4957
Yamanaka, Eric Seiti
author_role author
author2 Maquieira, Angel|||0000-0003-4641-4957
Yamanaka, Eric Seiti
author2_role author
author
dc.contributor.none.fl_str_mv Departamento de Química
Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Generalitat Valenciana
Ministerio de Economía y Competitividad
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Single-nucleotide polymorphism
Isothermal DNA amplification
Point-of-care testing
Smartphone
Scanner
Compact disc
QUIMICA ANALITICA
topic Single-nucleotide polymorphism
Isothermal DNA amplification
Point-of-care testing
Smartphone
Scanner
Compact disc
QUIMICA ANALITICA
description [EN] Consumer electronic technologies offer practical performances to develop compact biosensing systems intended for the point-of-care testing of DNA biomarkers. Herein a discrimination method for detecting single nucleotide polymorphisms, based on isothermal amplification and on-chip hybridisation, was developed and integrated into user-friendly optical devices: e.g., USB digital microscope, flatbed scanner, smartphone and DVD drive. In order to adequately identify a single base change, loop-mediated isothermal amplification (LAMP) was employed, with high yields (8 orders) within 45 min. Subsequently, products were directly hybridised to the allele-specific probes attached to plastic chips in an array format. After colorimetric staining, four consumer electronic techniques were compared. Sensitive precise measurements were taken (high signal-to-noise ratios, 10-mu m image resolution, 99% scan-to-scan reproducibility). These features confirmed their potential as analytical tools, are a competitive alternative to fluorescence scanners, and incorporate additional advantages, such as user-friendly interface and connectivity for telemedicine needs. The analytical performances of the integrated platform (assay and reader) in the human samples were also excellent, with a low detection limit (100 genomic DNA copies), and reproducible (< 15%) and cheap assays (< 10 (sic)/test). The correct genotyping of a genetic biomarker (single-nucleotide polymorphism located in the GRIK4 gene) was achieved as the assigned genotypes agreed with those determined by using sequencing. The portability, favourable discriminating and read-out capabilities reveal that the implementation of mass-produced low-cost devices into minimal-specialised clinical laboratories is closer to becoming a reality.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/141451
url https://riunet.upv.es/handle/10251/141451
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Generalitat Valenciana https://doi.org/10.13039/501100003359 GRISOLIA%2F2014%2F024
Generalitat Valenciana https://doi.org/10.13039/501100003359 PROMETEOII%2F2014%2F040 Estudio de estrategias fisico-químicas para el desarrollo de biosensores interferométricos en soportes interactivos de aplicación en clínica
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 CTQ2016-75749-R BIOSENSORES HOLOGRAFICOS. PRUEBA DE CONCEPTO Y DEMOSTRACION EN APLICACIONES CLINICAS
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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