Molecular analysis of menadione-induced resistance against biotic stress in Arabidopsis

19 páginas, 6 figuras, 2 tablas.

Detalles Bibliográficos
Autores: Borges, Andrés A., Dobón, Albor, Expósito Rodríguez, Marino, Jiménez Arias, David, Borges-Pérez, Andrés, Casañas-Sánchez, Verónica, Pérez Méndez, J. A., Luis Jorge, Juan Cristo, Tornero, Pablo
Tipo de recurso: artículo
Fecha de publicación:2009
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/42861
Acceso en línea:http://hdl.handle.net/10261/42861
Access Level:acceso abierto
Palabra clave:Arabidopsis
Menadione sodium bisulphite
Induced resistance
Priming
Pseudomonas
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spelling Molecular analysis of menadione-induced resistance against biotic stress in ArabidopsisBorges, Andrés A.Dobón, AlborExpósito Rodríguez, MarinoJiménez Arias, DavidBorges-Pérez, AndrésCasañas-Sánchez, VerónicaPérez Méndez, J. A.Luis Jorge, Juan CristoTornero, PabloArabidopsisMenadione sodium bisulphiteInduced resistancePrimingPseudomonas19 páginas, 6 figuras, 2 tablas.Menadione sodium bisulphite (MSB) is a water-soluble derivative of vitamin K3, or menadione, and has been previously demonstrated to function as a plant defence activator against several pathogens in several plant species. However, there are no reports of the role of this vitamin in the induction of resistance in the plant model Arabidopsis thaliana. In the current study, we demonstrate that MSB induces resistance by priming in Arabidopsis against the virulent strain Pseudomonas syringae pv. tomato DC3000 (Pto) without inducing necrosis or visible damage. Changes in gene expression in response to 0.2 mm MSB were analysed in Arabidopsis at 3, 6 and 24 h post-treatment using microarray technology. In general, the treatment with MSB does not correlate with other publicly available data, thus MSB produces a unique molecular footprint. We observed 158 differentially regulated genes among all the possible trends. More up-regulated genes are included in categories such as 'response to stress' than the background, and the behaviour of these genes in different treatments confirms their role in response to biotic and abiotic stress. In addition, there is an over-representation of the G-box in their promoters. Some interesting functions are represented among the individual up-regulated genes, such as glutathione S-transferases, transcription factors (including putative regulators of the G-box) and cytochrome P450s. This work provides a wide insight into the molecular cues underlying the effect of MSB as a plant resistance inducer.This work was partially funded by an INVESCAN, S.L. grant (No.OTT2001438) to the CSIC and by a BIO2006-02168 grant of MICINN to PT. The microarrays were funded in part by the “Genome España” Foundation. MER was supported by a research contract (ID-TF-06/002) from the Consejería de Industria, Comercio y Nuevas Tecnologías (Gobierno de Canarias). The authors thank CajaCanarias for their research support. We also thank Lorena Perales for her help in performing the bacterial growth curves, Dr. Héctor Cabrera for his useful advice on writing the manuscript, the English translation service of the Universidad Politécnica de Valencia and Mrs. Pauline Agnew whose endeavoured to edit the English translation of this paper.Peer reviewedBlackwell PublishingInvescan ConsultoresMinisterio de Ciencia e Innovación (España)Fundación Genoma EspañaCaja CanariasGobierno de Canarias201120112009info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/42861reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1111/j.1467-7652.2009.00439.xinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/428612026-05-22T06:33:51Z
dc.title.none.fl_str_mv Molecular analysis of menadione-induced resistance against biotic stress in Arabidopsis
title Molecular analysis of menadione-induced resistance against biotic stress in Arabidopsis
spellingShingle Molecular analysis of menadione-induced resistance against biotic stress in Arabidopsis
Borges, Andrés A.
Arabidopsis
Menadione sodium bisulphite
Induced resistance
Priming
Pseudomonas
title_short Molecular analysis of menadione-induced resistance against biotic stress in Arabidopsis
title_full Molecular analysis of menadione-induced resistance against biotic stress in Arabidopsis
title_fullStr Molecular analysis of menadione-induced resistance against biotic stress in Arabidopsis
title_full_unstemmed Molecular analysis of menadione-induced resistance against biotic stress in Arabidopsis
title_sort Molecular analysis of menadione-induced resistance against biotic stress in Arabidopsis
dc.creator.none.fl_str_mv Borges, Andrés A.
Dobón, Albor
Expósito Rodríguez, Marino
Jiménez Arias, David
Borges-Pérez, Andrés
Casañas-Sánchez, Verónica
Pérez Méndez, J. A.
Luis Jorge, Juan Cristo
Tornero, Pablo
author Borges, Andrés A.
author_facet Borges, Andrés A.
Dobón, Albor
Expósito Rodríguez, Marino
Jiménez Arias, David
Borges-Pérez, Andrés
Casañas-Sánchez, Verónica
Pérez Méndez, J. A.
Luis Jorge, Juan Cristo
Tornero, Pablo
author_role author
author2 Dobón, Albor
Expósito Rodríguez, Marino
Jiménez Arias, David
Borges-Pérez, Andrés
Casañas-Sánchez, Verónica
Pérez Méndez, J. A.
Luis Jorge, Juan Cristo
Tornero, Pablo
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Invescan Consultores
Ministerio de Ciencia e Innovación (España)
Fundación Genoma España
Caja Canarias
Gobierno de Canarias
dc.subject.none.fl_str_mv Arabidopsis
Menadione sodium bisulphite
Induced resistance
Priming
Pseudomonas
topic Arabidopsis
Menadione sodium bisulphite
Induced resistance
Priming
Pseudomonas
description 19 páginas, 6 figuras, 2 tablas.
publishDate 2009
dc.date.none.fl_str_mv 2009
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/42861
url http://hdl.handle.net/10261/42861
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1111/j.1467-7652.2009.00439.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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