Histone carbonylation occurs in proliferating cells

12 páginas, 10 figuras (que no es encuentran en este documento, se pueden ver en: http://www.sciencedirect.com/science/article/pii/S0891584912000664)

Detalles Bibliográficos
Autores: García-Giménez, José Luis, Velázquez Ledesma, Ana María, Esmoris, Isabel, Romá-Mateo, Carlos, Sanz, Pascual, Viña, José, Pallardó, Federico V.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2012
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/116940
Acceso en línea:http://hdl.handle.net/10261/116940
Access Level:acceso abierto
Palabra clave:Epigenetics
Histones
Carbonylation
Poly(ADP-ribosyl)ation
Cell proliferation
Free radicals
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spelling Histone carbonylation occurs in proliferating cellsGarcía-Giménez, José LuisVelázquez Ledesma, Ana MaríaEsmoris, IsabelRomá-Mateo, CarlosSanz, PascualViña, JoséPallardó, Federico V.EpigeneticsHistonesCarbonylationPoly(ADP-ribosyl)ationCell proliferationFree radicals12 páginas, 10 figuras (que no es encuentran en este documento, se pueden ver en: http://www.sciencedirect.com/science/article/pii/S0891584912000664)Chromatin is a dynamic structure formed mainly by DNA and histones, and chemical modifications on these elements regulate its compaction. Histone posttranslational modifications (PTMs) have a direct impact on chromatin conformation, controlling important cellular events such as cell proliferation and differentiation. Redox-related posttranslational modifications may have important effects on chromatin structure and function, offering a new intriguing area of research termed "redox epigenetics." Little is known about histone carbonylation, a PTM that may be related to modifications in the cellular redox environment. The aim of our study was to determine the carbonylation of the various histones during cell proliferation, a moment in cell life during which important redox changes take place. Here, we describe changes in histone carbonylation during cell proliferation in NIH3T3 fibroblasts. In addition, we have studied the variations of poly(ADP-ribosyl)ation and phospho-H2AX at the same time, because both modifications are related to DNA damage responses. High levels of carbonylation on specific histones (H1, H1(0), and H3.1 dimers) were found when cells were in an active phase of DNA synthesis. The modification decreased when nuclear proteasome activity was activated. However, these results did not correlate completely with poly(ADP-ribosyl)ation and phospho-H2AX levels. Therefore, histone carbonylation may represent a specific event during cell proliferation. We describe a new methodology named oxy-2D-TAU Western blot that allowed us to separate and analyze the carbonylation patterns of the histone variants. In addition we offer a new role for histone carbonylation and its implication in redox epigenetics. Our results suggest that histone carbonylation is involved in histone detoxification during DNA synthesisThis work was supported by SAF2008-01338 grants from the Ministerio de Ciencia e Innovación and financial support from the CIBERER (Biomedical Network Research Center for Rare Diseases) and the ISCIII to FVP. JLG-G thanks the Conselleria de Sanitat de la Generalitat Valenciana for the grant which allowed his stay in Hake’s laboratory.Peer reviewedElsevierConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201520152012info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/116940reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.freeradbiomed.2012.01.022Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1169402026-05-22T06:33:51Z
dc.title.none.fl_str_mv Histone carbonylation occurs in proliferating cells
title Histone carbonylation occurs in proliferating cells
spellingShingle Histone carbonylation occurs in proliferating cells
García-Giménez, José Luis
Epigenetics
Histones
Carbonylation
Poly(ADP-ribosyl)ation
Cell proliferation
Free radicals
title_short Histone carbonylation occurs in proliferating cells
title_full Histone carbonylation occurs in proliferating cells
title_fullStr Histone carbonylation occurs in proliferating cells
title_full_unstemmed Histone carbonylation occurs in proliferating cells
title_sort Histone carbonylation occurs in proliferating cells
dc.creator.none.fl_str_mv García-Giménez, José Luis
Velázquez Ledesma, Ana María
Esmoris, Isabel
Romá-Mateo, Carlos
Sanz, Pascual
Viña, José
Pallardó, Federico V.
author García-Giménez, José Luis
author_facet García-Giménez, José Luis
Velázquez Ledesma, Ana María
Esmoris, Isabel
Romá-Mateo, Carlos
Sanz, Pascual
Viña, José
Pallardó, Federico V.
author_role author
author2 Velázquez Ledesma, Ana María
Esmoris, Isabel
Romá-Mateo, Carlos
Sanz, Pascual
Viña, José
Pallardó, Federico V.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Epigenetics
Histones
Carbonylation
Poly(ADP-ribosyl)ation
Cell proliferation
Free radicals
topic Epigenetics
Histones
Carbonylation
Poly(ADP-ribosyl)ation
Cell proliferation
Free radicals
description 12 páginas, 10 figuras (que no es encuentran en este documento, se pueden ver en: http://www.sciencedirect.com/science/article/pii/S0891584912000664)
publishDate 2012
dc.date.none.fl_str_mv 2012
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/116940
url http://hdl.handle.net/10261/116940
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.freeradbiomed.2012.01.022
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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