Intestinal anti-inflammatory effects of oligosaccharides derived from lactulose in the trinitrobenzenesulfonic acid model of rat colitis

Intestinal microbiota modulation is becoming an interesting approach to manage inflammatory bowel disease and can be achieved by the administration of prebiotics. Previous studies showed the intestinal anti-inflammatory effects of the prebiotic lactulose. The aim of the present study was to test the...

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Autores: Algieri, Francesca, Rodríguez-Nogales, Alba, Garrido-Mesa, Natividad, Vezza, Teresa, Garrido-Mesa, José, Utrilla, Pilar M., Montilla, Antonia, Cardelle-Cobas, Alejandra, Olano, Agustín, Corzo, Nieves, Guerra-Hernández, Eduardo, Zarzuelo, Antonio, Rodriguez-Cabezas, M. Elena, Gálvez, Julio
Tipo de recurso: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/100082
Acceso en línea:http://hdl.handle.net/10261/100082
Access Level:acceso abierto
Palabra clave:Prebiotic
Lactulose-derived oligosaccharides
Intestinal anti-inflammatory effect
TNBS rat colitis
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spelling Intestinal anti-inflammatory effects of oligosaccharides derived from lactulose in the trinitrobenzenesulfonic acid model of rat colitisAlgieri, FrancescaRodríguez-Nogales, AlbaGarrido-Mesa, NatividadVezza, TeresaGarrido-Mesa, JoséUtrilla, Pilar M.Montilla, AntoniaCardelle-Cobas, AlejandraOlano, AgustínCorzo, NievesGuerra-Hernández, EduardoZarzuelo, AntonioRodriguez-Cabezas, M. ElenaGálvez, JulioPrebioticLactulose-derived oligosaccharidesIntestinal anti-inflammatory effectTNBS rat colitisIntestinal microbiota modulation is becoming an interesting approach to manage inflammatory bowel disease and can be achieved by the administration of prebiotics. Previous studies showed the intestinal anti-inflammatory effects of the prebiotic lactulose. The aim of the present study was to test the preventative effects of oligosaccharides derived from lactulose with prebiotic properties (OsLu) in the trinitrobenzenesulfonic acid model of rat colitis and compare them with those of lactulose. Both treatments modified bacterial profile in intestinal contents, increasing the bifidobacteria and lactobacilli counts and up-regulating the production of short-chain fatty acids, although OsLu generated a larger amount. OsLu also inhibited to a greater extent different pro-inflammatory markers such as interleukins (IL) 1, 6, 12, and 23 and chemokines (MCP-1 and CINC-1). However, both prebiotics equally restored colonic epithelial integrity, evaluated both with a histological score (OsLu, 9.8 ± 2.2; and lactulose, 12.1 ± 2.1, vs colitic control, 27.3 ± 3.3) and by measuring several key proteins of the mucosal barrier (MUC-2, MUC-3, and TTF-3). OsLu effect was also associated with an inhibition of iNOS expression and a reduction of Th17 cell activity in the inflamed tissue that facilitated the intestinal mucosa barrier recovery. In conclusion, OsLu showed a better anti-inflammatory profile than lactulose in this model of experimental colitis. © 2014 American Chemical Society.This work was supported by the Spanish Ministry of Economy and Competitivity (SAF2011-29648), by Consolider-Ingenio 2010: FUNC-C-FOOD CSD 2007-00063, and by Junta de Andalucia (AGR-6826 and CTS 164) with funds from the European Union. F.A. is a predoctoral fellow of the Junta de Andalucia; J.G.-M. is a predoctoral fellow of the Spanish Ministry of Education and Science; N.G.-M. is a postdoctoral fellow of the Ramon Areces Foundation; M.E.R.-C. is a postdoctoral fellow of CIBEREHD. CIBEREHD is funded by the Instituto de Salud Carlos III.Peer ReviewedAmerican Chemical SocietyMinisterio de Economía y Competitividad (España)Ministerio de Educación y Ciencia (España)Junta de AndalucíaEuropean CommissionFundación Ramón ArecesInstituto de Salud Carlos IIIConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2014201420142014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Preprintinfo:eu-repo/semantics/submittedVersionhttp://hdl.handle.net/10261/100082reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1021/jf500678pSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1000822026-05-22T06:33:51Z
dc.title.none.fl_str_mv Intestinal anti-inflammatory effects of oligosaccharides derived from lactulose in the trinitrobenzenesulfonic acid model of rat colitis
title Intestinal anti-inflammatory effects of oligosaccharides derived from lactulose in the trinitrobenzenesulfonic acid model of rat colitis
spellingShingle Intestinal anti-inflammatory effects of oligosaccharides derived from lactulose in the trinitrobenzenesulfonic acid model of rat colitis
Algieri, Francesca
Prebiotic
Lactulose-derived oligosaccharides
Intestinal anti-inflammatory effect
TNBS rat colitis
title_short Intestinal anti-inflammatory effects of oligosaccharides derived from lactulose in the trinitrobenzenesulfonic acid model of rat colitis
title_full Intestinal anti-inflammatory effects of oligosaccharides derived from lactulose in the trinitrobenzenesulfonic acid model of rat colitis
title_fullStr Intestinal anti-inflammatory effects of oligosaccharides derived from lactulose in the trinitrobenzenesulfonic acid model of rat colitis
title_full_unstemmed Intestinal anti-inflammatory effects of oligosaccharides derived from lactulose in the trinitrobenzenesulfonic acid model of rat colitis
title_sort Intestinal anti-inflammatory effects of oligosaccharides derived from lactulose in the trinitrobenzenesulfonic acid model of rat colitis
dc.creator.none.fl_str_mv Algieri, Francesca
Rodríguez-Nogales, Alba
Garrido-Mesa, Natividad
Vezza, Teresa
Garrido-Mesa, José
Utrilla, Pilar M.
Montilla, Antonia
Cardelle-Cobas, Alejandra
Olano, Agustín
Corzo, Nieves
Guerra-Hernández, Eduardo
Zarzuelo, Antonio
Rodriguez-Cabezas, M. Elena
Gálvez, Julio
author Algieri, Francesca
author_facet Algieri, Francesca
Rodríguez-Nogales, Alba
Garrido-Mesa, Natividad
Vezza, Teresa
Garrido-Mesa, José
Utrilla, Pilar M.
Montilla, Antonia
Cardelle-Cobas, Alejandra
Olano, Agustín
Corzo, Nieves
Guerra-Hernández, Eduardo
Zarzuelo, Antonio
Rodriguez-Cabezas, M. Elena
Gálvez, Julio
author_role author
author2 Rodríguez-Nogales, Alba
Garrido-Mesa, Natividad
Vezza, Teresa
Garrido-Mesa, José
Utrilla, Pilar M.
Montilla, Antonia
Cardelle-Cobas, Alejandra
Olano, Agustín
Corzo, Nieves
Guerra-Hernández, Eduardo
Zarzuelo, Antonio
Rodriguez-Cabezas, M. Elena
Gálvez, Julio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Ministerio de Educación y Ciencia (España)
Junta de Andalucía
European Commission
Fundación Ramón Areces
Instituto de Salud Carlos III
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Prebiotic
Lactulose-derived oligosaccharides
Intestinal anti-inflammatory effect
TNBS rat colitis
topic Prebiotic
Lactulose-derived oligosaccharides
Intestinal anti-inflammatory effect
TNBS rat colitis
description Intestinal microbiota modulation is becoming an interesting approach to manage inflammatory bowel disease and can be achieved by the administration of prebiotics. Previous studies showed the intestinal anti-inflammatory effects of the prebiotic lactulose. The aim of the present study was to test the preventative effects of oligosaccharides derived from lactulose with prebiotic properties (OsLu) in the trinitrobenzenesulfonic acid model of rat colitis and compare them with those of lactulose. Both treatments modified bacterial profile in intestinal contents, increasing the bifidobacteria and lactobacilli counts and up-regulating the production of short-chain fatty acids, although OsLu generated a larger amount. OsLu also inhibited to a greater extent different pro-inflammatory markers such as interleukins (IL) 1, 6, 12, and 23 and chemokines (MCP-1 and CINC-1). However, both prebiotics equally restored colonic epithelial integrity, evaluated both with a histological score (OsLu, 9.8 ± 2.2; and lactulose, 12.1 ± 2.1, vs colitic control, 27.3 ± 3.3) and by measuring several key proteins of the mucosal barrier (MUC-2, MUC-3, and TTF-3). OsLu effect was also associated with an inhibition of iNOS expression and a reduction of Th17 cell activity in the inflamed tissue that facilitated the intestinal mucosa barrier recovery. In conclusion, OsLu showed a better anti-inflammatory profile than lactulose in this model of experimental colitis. © 2014 American Chemical Society.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Preprint
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/100082
url http://hdl.handle.net/10261/100082
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1021/jf500678p
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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