Size-fractionated phytoplankton diversity in the NW Iberian coast: a combination of microscopic, pigment and molecular analyses

11 pages, 5 figures, 1 table

Detalles Bibliográficos
Autores: Not, Fabrice, Zapata, Manuel, Pazos, Yolanda, Campaña, Emilia, Doval, M. Dolores, Rodríguez Hernández, Francisco José
Tipo de recurso: artículo
Fecha de publicación:2007
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/116240
Acceso en línea:http://hdl.handle.net/10261/116240
Access Level:acceso abierto
Palabra clave:FISH-TSA
Pigments
Microscopy
Picoplankton
Ría de Pontevedra
Phytoplankton diversity
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spelling Size-fractionated phytoplankton diversity in the NW Iberian coast: a combination of microscopic, pigment and molecular analysesNot, FabriceZapata, ManuelPazos, YolandaCampaña, EmiliaDoval, M. DoloresRodríguez Hernández, Francisco JoséFISH-TSAPigmentsMicroscopyPicoplanktonRía de PontevedraPhytoplankton diversity11 pages, 5 figures, 1 tableThe aim of our study was to describe the diversity and dynamics of size-fractionated phytoplankton at a fixed station (P2, Ría de Pontevedra, NW Spain) from late spring to early winter. Phytoplankton successions were assessed weekly from May to November 2004 using pigment analyses, light microscopy and fluorescence in situ hybridization coupled with tyramide signal amplification (FISH-TSA). Periodic upwelling events triggered the development of diatom populations followed by periods of relaxation/downwelling conditions where remineralization processes (maxima of NH4 +), raised temperatures and/or low salinity waters from rainfall favored the increase of picophytoplankton. Marked differences were observed between diversity and dynamics of the micronano (>3 μm) and pico (<3 pm) size fractions. Micro-nanoplankton organisms contributed 85 % of chl a on average (range, 55 to 99 %); diatoms and cryptophytes were the dominant pigment groups (∼70 % chl a in this size category). In the picoplankton, chlorophytes and non-chlorophytes contributed to an identical proportion (50 %) of total picoeukaryotes determined by FISH-TSA. A large fraction of these (∼65 %) could be accounted for by group-specific probes. Molecular data suggested that chl b-containing picoplankton were mostly accounted for by prasinophyceans belonging to the order Mamiellales (55 % of total chlorophyte cells), with Micromonas pusilla as its major component (∼70 % of Mamiellales, ∼20 % of all picoeukaryotes), similar to that found in other temperate coastal locations. In contrast, HPLC analyses severely underestimated the contribution of prasinophyceans and indicated <10 % Mamiellales among chlorophytes. Among chromophytes, the haptophyte probe targeted ∼25 % of non-chlorophyte cells, and pelagophyceans were sporadically abundant (13 % non-chlorophyte cells, May to September). After pigment analyses the main chromophytes were diatom II (chl c3-containing), pelagophyceans and haptophytes with 20, 13 and 8 % of picoplanktonic chl a, respectively. © Inter-Research 2007F.R. was supported by a postdoctoral fellowship from Caixanova FoundationPeer ReviewedInter Research2015201520072015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/116240reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3354/ame01144info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1162402026-05-22T06:33:51Z
dc.title.none.fl_str_mv Size-fractionated phytoplankton diversity in the NW Iberian coast: a combination of microscopic, pigment and molecular analyses
title Size-fractionated phytoplankton diversity in the NW Iberian coast: a combination of microscopic, pigment and molecular analyses
spellingShingle Size-fractionated phytoplankton diversity in the NW Iberian coast: a combination of microscopic, pigment and molecular analyses
Not, Fabrice
FISH-TSA
Pigments
Microscopy
Picoplankton
Ría de Pontevedra
Phytoplankton diversity
title_short Size-fractionated phytoplankton diversity in the NW Iberian coast: a combination of microscopic, pigment and molecular analyses
title_full Size-fractionated phytoplankton diversity in the NW Iberian coast: a combination of microscopic, pigment and molecular analyses
title_fullStr Size-fractionated phytoplankton diversity in the NW Iberian coast: a combination of microscopic, pigment and molecular analyses
title_full_unstemmed Size-fractionated phytoplankton diversity in the NW Iberian coast: a combination of microscopic, pigment and molecular analyses
title_sort Size-fractionated phytoplankton diversity in the NW Iberian coast: a combination of microscopic, pigment and molecular analyses
dc.creator.none.fl_str_mv Not, Fabrice
Zapata, Manuel
Pazos, Yolanda
Campaña, Emilia
Doval, M. Dolores
Rodríguez Hernández, Francisco José
author Not, Fabrice
author_facet Not, Fabrice
Zapata, Manuel
Pazos, Yolanda
Campaña, Emilia
Doval, M. Dolores
Rodríguez Hernández, Francisco José
author_role author
author2 Zapata, Manuel
Pazos, Yolanda
Campaña, Emilia
Doval, M. Dolores
Rodríguez Hernández, Francisco José
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv FISH-TSA
Pigments
Microscopy
Picoplankton
Ría de Pontevedra
Phytoplankton diversity
topic FISH-TSA
Pigments
Microscopy
Picoplankton
Ría de Pontevedra
Phytoplankton diversity
description 11 pages, 5 figures, 1 table
publishDate 2007
dc.date.none.fl_str_mv 2007
2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/116240
url http://hdl.handle.net/10261/116240
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3354/ame01144
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Inter Research
publisher.none.fl_str_mv Inter Research
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869407633361862656
score 15,81155

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