A bioinspired peptide scaffold with high antibiotic activity and low in vivo toxicity

Bacterial resistance to almost all available antibiotics is an important public health issue. A major goal in antimicrobial drug discovery is the generation of new chemicals capable of killing pathogens with high selectivity, particularly multi-drug-resistant ones. Here we report the design, prepara...

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Detalhes bibliográficos
Autores: Rabanal Anglada, Francesc, Grau Campistany, Ariadna, Vila Farrés, Xavier, González-Linares, J. (Javier), Borràs Suárez, Miquel, Vila Estapé, Jordi, Manresa Presas, Ma. Ángeles (María Ángeles), Cajal Visa, Yolanda
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/66210
Acesso em linha:https://hdl.handle.net/2445/66210
Access Level:acceso abierto
Palavra-chave:Desenvolupament de medicaments
Membranes (Biologia)
Pèptids
Toxicitat dels medicaments
Antibiòtics
Bacteris patògens
Drug development
Membranes (Biology)
Peptides
Drug toxicity
Antibiotics
Pathogenic bacteria
Descrição
Resumo:Bacterial resistance to almost all available antibiotics is an important public health issue. A major goal in antimicrobial drug discovery is the generation of new chemicals capable of killing pathogens with high selectivity, particularly multi-drug-resistant ones. Here we report the design, preparation and activity of new compounds based on a tunable, chemically accessible and upscalable lipopeptide scaffold amenable to suitable hit-to-lead development. Such compounds could become therapeutic candidates and future antibiotics available on the market. The compounds are cyclic, contain two D-amino acids for in vivo stability and their structures are reminiscent of other cyclic disulfide-containing peptides available on the market. The optimized compounds prove to be highly active against clinically relevant Gram-negative and Gram-positive bacteria. In vitro and in vivo tests show the low toxicity of the compounds. Their antimicrobial activity against resistant and multidrug-resistant bacteria is at the membrane level, although other targets may also be involved depending on the bacterial strain.