Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing Loss

Age-related hearing loss (ARHL) is an increasing and gradual sensorineural hearing dysfunction. Oxidative stress is an essential factor in developing ARHL; additionally, premature senescence of auditory cells induced by oxidative stress can produce hearing loss. Hydrogen peroxide (H2O2) represents a...

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Autores: Rivas Chacón, Luz del Mar, Martínez Rodríguez, Sofía, Madrid García, Raquel, Yanes Díaz, Joaquín, Riestra Ayora, Juan Ignacio, Sanz Fernández, Ricardo, Sánchez Rodríguez, Carolina
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Europea (UEM)
Repositorio:ABACUS. Repositorio de Producción Científica
Idioma:inglés
OAI Identifier:oai:abacus.universidadeuropea.com:11268/11982
Acceso en línea:http://hdl.handle.net/11268/11982
Access Level:acceso abierto
Palabra clave:Pérdida auditiva
Estrés oxidativo
Envejecimiento
Recepción auditiva
Tratamiento médico
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spelling Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing LossRivas Chacón, Luz del MarMartínez Rodríguez, SofíaMadrid García, RaquelYanes Díaz, JoaquínRiestra Ayora, Juan IgnacioSanz Fernández, RicardoSánchez Rodríguez, CarolinaPérdida auditivaEstrés oxidativoEnvejecimientoRecepción auditivaTratamiento médicoAge-related hearing loss (ARHL) is an increasing and gradual sensorineural hearing dysfunction. Oxidative stress is an essential factor in developing ARHL; additionally, premature senescence of auditory cells induced by oxidative stress can produce hearing loss. Hydrogen peroxide (H2O2) represents a method commonly used to generate cellular senescence in vitro. The objective of the present paper is to study H2O2-induced senescence patterns in three auditory cell lines (House Ear Institute-Organ of Corti 1, HEI-OC1; organ of Corti, OC-k3, and stria vascularis, SV-k1 cells) to elucidate the intrinsic mechanisms responsible for ARHL. The auditory cells were exposed to H2O2 at different concentrations and times. The results obtained show different responses of the hearing cells concerning cell growth, β-galactosidase activity, morphological changes, mitochondrial activation, levels of oxidative stress, and other markers of cell damage (Forkhead box O3a, FoxO3a, and 8-oxoguanine, 8-oxoG). Comparison between the responses of these auditory cells to H2O2 is a helpful method to evaluate the molecular mechanisms responsible for these auditory cells’ senescence. Furthermore, this in vitro model could help develop anti-senescent therapeutic strategies for the treatment of AHRL.20232023-03-3120212021-01-0120212021-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/11268/11982reponame:ABACUS. Repositorio de Producción Científicainstname:Universidad Europea (UEM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:abacus.universidadeuropea.com:11268/119822026-06-11T12:41:27Z
dc.title.none.fl_str_mv Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing Loss
title Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing Loss
spellingShingle Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing Loss
Rivas Chacón, Luz del Mar
Pérdida auditiva
Estrés oxidativo
Envejecimiento
Recepción auditiva
Tratamiento médico
title_short Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing Loss
title_full Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing Loss
title_fullStr Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing Loss
title_full_unstemmed Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing Loss
title_sort Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing Loss
dc.creator.none.fl_str_mv Rivas Chacón, Luz del Mar
Martínez Rodríguez, Sofía
Madrid García, Raquel
Yanes Díaz, Joaquín
Riestra Ayora, Juan Ignacio
Sanz Fernández, Ricardo
Sánchez Rodríguez, Carolina
author Rivas Chacón, Luz del Mar
author_facet Rivas Chacón, Luz del Mar
Martínez Rodríguez, Sofía
Madrid García, Raquel
Yanes Díaz, Joaquín
Riestra Ayora, Juan Ignacio
Sanz Fernández, Ricardo
Sánchez Rodríguez, Carolina
author_role author
author2 Martínez Rodríguez, Sofía
Madrid García, Raquel
Yanes Díaz, Joaquín
Riestra Ayora, Juan Ignacio
Sanz Fernández, Ricardo
Sánchez Rodríguez, Carolina
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Pérdida auditiva
Estrés oxidativo
Envejecimiento
Recepción auditiva
Tratamiento médico
topic Pérdida auditiva
Estrés oxidativo
Envejecimiento
Recepción auditiva
Tratamiento médico
description Age-related hearing loss (ARHL) is an increasing and gradual sensorineural hearing dysfunction. Oxidative stress is an essential factor in developing ARHL; additionally, premature senescence of auditory cells induced by oxidative stress can produce hearing loss. Hydrogen peroxide (H2O2) represents a method commonly used to generate cellular senescence in vitro. The objective of the present paper is to study H2O2-induced senescence patterns in three auditory cell lines (House Ear Institute-Organ of Corti 1, HEI-OC1; organ of Corti, OC-k3, and stria vascularis, SV-k1 cells) to elucidate the intrinsic mechanisms responsible for ARHL. The auditory cells were exposed to H2O2 at different concentrations and times. The results obtained show different responses of the hearing cells concerning cell growth, β-galactosidase activity, morphological changes, mitochondrial activation, levels of oxidative stress, and other markers of cell damage (Forkhead box O3a, FoxO3a, and 8-oxoguanine, 8-oxoG). Comparison between the responses of these auditory cells to H2O2 is a helpful method to evaluate the molecular mechanisms responsible for these auditory cells’ senescence. Furthermore, this in vitro model could help develop anti-senescent therapeutic strategies for the treatment of AHRL.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01
2021
2021-01-01
2023
2023-03-31
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/11268/11982
url http://hdl.handle.net/11268/11982
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:ABACUS. Repositorio de Producción Científica
instname:Universidad Europea (UEM)
instname_str Universidad Europea (UEM)
reponame_str ABACUS. Repositorio de Producción Científica
collection ABACUS. Repositorio de Producción Científica
repository.name.fl_str_mv
repository.mail.fl_str_mv
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