Serological diagnosis of canine leishmaniosis
Speed Leish K ® is used as a serological screening test for Leishmania infection prior to vaccination. Limited comparative serological studies with Speed Leish K ® have been performed. The aim of this study was to evaluate the diagnostic performance of four commercially available serologic tests inc...
| Autores: | , , , , , |
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| Formato: | artículo |
| Fecha de publicación: | 2014 |
| País: | España |
| Recursos: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:185018 |
| Acesso em linha: | https://ddd.uab.cat/record/185018 https://dx.doi.org/urn:doi:10.1186/1756-3305-7-111 |
| Access Level: | acceso abierto |
| Palavra-chave: | Leishmania infantum Dog ELISA IFAT Serological rapid test and vaccine |
| Resumo: | Speed Leish K ® is used as a serological screening test for Leishmania infection prior to vaccination. Limited comparative serological studies with Speed Leish K ® have been performed. The aim of this study was to evaluate the diagnostic performance of four commercially available serologic tests including ELISAs (Leiscan ®, ID Screen ® and Leishmania 96 ®), a rapid test (Speed Leish K ®) and an in-house IFAT for the detection of specific antibodies against Leishmania infantum antigen in dogs in different states of infection. Sick infected dogs (n = 36), healthy infected dogs (n = 18), L. infantum seropositive dogs with low to high levels of antibodies (n = 53), dogs seropositive to other pathogens (to evaluate cross reaction) (n = 14) and uninfected dogs from a non-endemic area (n = 50) and from an endemic area (n = 32) were analysed by the serological methods mentioned above. The sensitivity was as follows: ID Screen ® (0.953), Leiscan ® and Leishmania 96 ® (0.925), IFAT (0.869) and Speed Leish K ® (0.636). The maximum specificity (1.000) was attained for all diagnostic tests except the Leishmania 96 ® (0.896) and IFAT (0.917). The accuracy was as follows: ID Screen ® (0.975), Leiscan ® (0.961), Leishmania 96 ® (0.911), IFAT (0.892) and Speed Leish K ® (0.808). In relation to the area under the ROC curve (AUC-ROC), the maximum value was attained with the ID Screen ® (0.993) closely followed by Leiscan ® (0.990), then, Leishmania 96 ® (0.962), IFAT (0.926) and Speed Leish K ® (0.818). For the Kappa index, the best result was obtained by the ID Screen ® (0.951) followed by Leiscan ® (0.921), Leishmania 96 ® (0.822), IFAT (0.783) and Speed Leish K ® (0.622). Statistically significant differences were found between the AUC-ROC of quantitative serological tests and the only qualitative rapid test evaluated. There were also statistically significant differences between AUC-ROC of the ELISAs (ID Screen ® and Leiscan ®) and IFAT. Leiscan ® and ID Screen ® had superior diagnostic performance measures than IFAT and all quantitative serological tests were superior when compared to Speed Leish K ®. Thus, Speed Leish K ® may be considered a less valuable screening test prior to vaccination as it may result in vaccination of seropositive dogs and in some cases seropositive sick dogs. |
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