In vitro response to EPA, DPA and DHA: comparison of effects on ruminal fermentation and biohydrogenation of 18-carbon fatty acids in cows and ewes

12 páginas, 3 tablas, 1 figura.

Detalles Bibliográficos
Autores: Toral, Pablo G., Hervás, Gonzalo, Carreño Yugueros, David, Leskinen, H., Belenguer, Álvaro, Shingfield, Kevin J., Frutos, Pilar
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/153273
Acceso en línea:http://hdl.handle.net/10261/153273
Access Level:acceso abierto
Palabra clave:Cattle
Polyunsaturated fatty acids
Ruminal lipid metabolism
Sheep
Trans fatty acid
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repository_id_str
dc.title.none.fl_str_mv In vitro response to EPA, DPA and DHA: comparison of effects on ruminal fermentation and biohydrogenation of 18-carbon fatty acids in cows and ewes
title In vitro response to EPA, DPA and DHA: comparison of effects on ruminal fermentation and biohydrogenation of 18-carbon fatty acids in cows and ewes
spellingShingle In vitro response to EPA, DPA and DHA: comparison of effects on ruminal fermentation and biohydrogenation of 18-carbon fatty acids in cows and ewes
Toral, Pablo G.
Cattle
Polyunsaturated fatty acids
Ruminal lipid metabolism
Sheep
Trans fatty acid
title_short In vitro response to EPA, DPA and DHA: comparison of effects on ruminal fermentation and biohydrogenation of 18-carbon fatty acids in cows and ewes
title_full In vitro response to EPA, DPA and DHA: comparison of effects on ruminal fermentation and biohydrogenation of 18-carbon fatty acids in cows and ewes
title_fullStr In vitro response to EPA, DPA and DHA: comparison of effects on ruminal fermentation and biohydrogenation of 18-carbon fatty acids in cows and ewes
title_full_unstemmed In vitro response to EPA, DPA and DHA: comparison of effects on ruminal fermentation and biohydrogenation of 18-carbon fatty acids in cows and ewes
title_sort In vitro response to EPA, DPA and DHA: comparison of effects on ruminal fermentation and biohydrogenation of 18-carbon fatty acids in cows and ewes
dc.creator.none.fl_str_mv Toral, Pablo G.
Hervás, Gonzalo
Carreño Yugueros, David
Leskinen, H.
Belenguer, Álvaro
Shingfield, Kevin J.
Frutos, Pilar
author Toral, Pablo G.
author_facet Toral, Pablo G.
Hervás, Gonzalo
Carreño Yugueros, David
Leskinen, H.
Belenguer, Álvaro
Shingfield, Kevin J.
Frutos, Pilar
author_role author
author2 Hervás, Gonzalo
Carreño Yugueros, David
Leskinen, H.
Belenguer, Álvaro
Shingfield, Kevin J.
Frutos, Pilar
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía, Industria y Competitividad (España)
Consejo Superior de Investigaciones Científicas (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cattle
Polyunsaturated fatty acids
Ruminal lipid metabolism
Sheep
Trans fatty acid
topic Cattle
Polyunsaturated fatty acids
Ruminal lipid metabolism
Sheep
Trans fatty acid
description 12 páginas, 3 tablas, 1 figura.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/153273
url http://hdl.handle.net/10261/153273
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-54587
http://dx.doi.org/10.3168/jds.2017-12638
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Dairy Science Association
publisher.none.fl_str_mv American Dairy Science Association
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling In vitro response to EPA, DPA and DHA: comparison of effects on ruminal fermentation and biohydrogenation of 18-carbon fatty acids in cows and ewesToral, Pablo G.Hervás, GonzaloCarreño Yugueros, DavidLeskinen, H.Belenguer, ÁlvaroShingfield, Kevin J.Frutos, PilarCattlePolyunsaturated fatty acidsRuminal lipid metabolismSheepTrans fatty acid12 páginas, 3 tablas, 1 figura.The modulation of milk fat nutritional quality through fish oil supplementation seems to be largely explained by the action of n-3 very long chain polyunsaturated fatty acids (PUFA) on ruminal biohydrogenation (BH) of C18 fatty acids (FA). However, relationships among this action, disappearance of those PUFA in the rumen, and potential detrimental consequences on ruminal fermentation remain uncertain. This study compared the effect of 20:5n-3 (eicosapentaenoic acid; EPA), 22:5n-3 (docosapentaenoic acid; DPA), and 22:6n-3 (docosahexaenoic acid; DHA) on rumen fermentation and BH of C18 FA and was conducted simultaneously in cows and sheep to provide novel insights into interspecies differences. The trial was performed in vitro using batch cultures of rumen microorganisms with inocula collected from cannulated cows and ewes. The PUFA were added at a dose of 2% incubated dry matter, and treatment effects on ruminal C18 FA concentrations, PUFA disappearances, and fermentation parameters (gas production, ammonia and volatile FA concentrations, and dry matter and neutral detergent fiber disappearances) were examined after 24 h of incubation. A principal component analysis suggested that responses to PUFA treatments explained most of the variability; those of ruminant species were of lower relevance. Overall, EPA and DHA were equally effective for inhibiting the saturation of trans-11 18:1 to 18:0 and had a similar influence on ruminal fermentation in cows and sheep (e.g., reductions in gas production and acetate:propionate ratio). Nevertheless, DHA further promoted alternative BH pathways that lead to trans-10 18:1 accumulation, and EPA seemed to have specific effects on 18:3n-3 metabolism. Only minor variations attributable to DPA were observed in the studied parameters, suggesting a low contribution of this FA to the action of marine lipids. Although most changes due to the added PUFA were comparable in bovine and ovine, there were also relevant specificities, such as a stronger inhibition of 18:0 formation in cows and a greater increase in 18:3n-3 metabolites in sheep. No direct relationship between in vitro disappearance of the incubated PUFA and effect on BH (in particular, inhibition of the last step) was found in either cows or ewes, calling into question a putative link between extent of disappearance and toxicity for microbiota. Conversely, an association between the influence of these PUFA on ruminal lipid metabolism and fermentation may exist in both species. In vivo verification of these findings would be advisable. Copyright © 2017 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.We dedicate this article to the memory of Kevin J. Shingfield, a renowned expert in ruminant lipid metabolism and, above all, a good friend of ours. This work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO; AGL2014-54587-R) and the Spanish National Research Council (CSIC; PIE201540E104). P. G. Toral benefits from a Ramón y Cajal research contract and D. Carreño benefits from an FPI predoctoral grant, both from MINECO. Cofunding by the European Regional Development Fund is also acknowledged.Peer reviewedAmerican Dairy Science AssociationMinisterio de Economía, Industria y Competitividad (España)Consejo Superior de Investigaciones Científicas (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201720172017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/153273reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-54587http://dx.doi.org/10.3168/jds.2017-12638Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1532732026-05-22T06:33:51Z
score 15.81155

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