MicroRNA profiling during mouse ventricular maturation: a role for miR-27 modulating Mef2c expression

Aims: Non-coding RNA has been recently demonstrated to be a novel mechanism for modulation of gene expression at the post-transcriptional level. The importance of microRNAs in the cardiovascular system is now apparent. Mutations of distinct microRNAs have provided evidence for fundamental roles of m...

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Autores: Chinchilla, Ana, Lozano-Velasco, Estefanía, Daimi, Houria, Esteban-Ruiz, Francisco José, Crist, Colin, Aránega, Amelia Eva, Franco, Diego
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:España
Recursos:Universidad de Jaén
Repositorio:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
OAI Identifier:oai:ruja.ujaen.es:10953/4010
Acesso em linha:https://academic.oup.com/cardiovascres/article/89/1/98/325183?login=true
https://hdl.handle.net/10953/4010
Access Level:acceso abierto
Palavra-chave:microRNAs
Cardiogenesis
Microarrays
Mef2c
miR-27b
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spelling MicroRNA profiling during mouse ventricular maturation: a role for miR-27 modulating Mef2c expressionChinchilla, AnaLozano-Velasco, EstefaníaDaimi, HouriaEsteban-Ruiz, Francisco JoséCrist, ColinAránega, Amelia EvaFranco, DiegomicroRNAsCardiogenesisMicroarraysMef2cmiR-27bAims: Non-coding RNA has been recently demonstrated to be a novel mechanism for modulation of gene expression at the post-transcriptional level. The importance of microRNAs in the cardiovascular system is now apparent. Mutations of distinct microRNAs have provided evidence for fundamental roles of microRNAs during cardiovascular development. However, there is limited information about global microRNA profiles during mouse heart development. In this study, we have gained insight from the expression profiles of microRNAs during mouse ventricular development by microarray and qRT-PCR analysis. Methods and results: Our microarray analysis reveals that relatively few microRNAs display either increasing or decreasing expression profiles during ventricular chamber formation. Interestingly, most of the differentially expressed microRNAs display a rather discrete peak of expression at particular developmental stages. Furthermore, we demonstrate that micro-RNA-27b (miR-27b) displays an overt myocardial expression during heart development and that the transcription factor-encoding gene Mef2c is an miR-27b target. Conclusion: Our data present a comprehensive profile of microRNA expression during ventricular maturation, providing an entry point for investigation of the functional roles of the most abundantly and differentially expressed microRNAs during cardiogenesisVI EU Integrated Project ‘Heart Failure and Cardiac Repair’ LSHM-CT-2005-018630; Junta de Andalucía Proyecto de Excelencia ‘Desarrollo Cardiovascular y Terapia Celular’ CTS-1614; Ministerio de Ciencia e Innovación MICINN BFU2009-11566 y MICINN BFU2008-01217; Junta de Andalucia CTS-446 y BIO-302Oxford202520252010info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://academic.oup.com/cardiovascres/article/89/1/98/325183?login=truehttps://hdl.handle.net/10953/4010reponame:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaéninstname:Universidad de JaénCardiovascular Research 2011, Vol 89, Issue 1http://creativecommons.org/publicdomain/zero/1.0/info:eu-repo/semantics/openAccessoai:ruja.ujaen.es:10953/40102026-06-24T12:41:07Z
dc.title.none.fl_str_mv MicroRNA profiling during mouse ventricular maturation: a role for miR-27 modulating Mef2c expression
title MicroRNA profiling during mouse ventricular maturation: a role for miR-27 modulating Mef2c expression
spellingShingle MicroRNA profiling during mouse ventricular maturation: a role for miR-27 modulating Mef2c expression
Chinchilla, Ana
microRNAs
Cardiogenesis
Microarrays
Mef2c
miR-27b
title_short MicroRNA profiling during mouse ventricular maturation: a role for miR-27 modulating Mef2c expression
title_full MicroRNA profiling during mouse ventricular maturation: a role for miR-27 modulating Mef2c expression
title_fullStr MicroRNA profiling during mouse ventricular maturation: a role for miR-27 modulating Mef2c expression
title_full_unstemmed MicroRNA profiling during mouse ventricular maturation: a role for miR-27 modulating Mef2c expression
title_sort MicroRNA profiling during mouse ventricular maturation: a role for miR-27 modulating Mef2c expression
dc.creator.none.fl_str_mv Chinchilla, Ana
Lozano-Velasco, Estefanía
Daimi, Houria
Esteban-Ruiz, Francisco José
Crist, Colin
Aránega, Amelia Eva
Franco, Diego
author Chinchilla, Ana
author_facet Chinchilla, Ana
Lozano-Velasco, Estefanía
Daimi, Houria
Esteban-Ruiz, Francisco José
Crist, Colin
Aránega, Amelia Eva
Franco, Diego
author_role author
author2 Lozano-Velasco, Estefanía
Daimi, Houria
Esteban-Ruiz, Francisco José
Crist, Colin
Aránega, Amelia Eva
Franco, Diego
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv microRNAs
Cardiogenesis
Microarrays
Mef2c
miR-27b
topic microRNAs
Cardiogenesis
Microarrays
Mef2c
miR-27b
description Aims: Non-coding RNA has been recently demonstrated to be a novel mechanism for modulation of gene expression at the post-transcriptional level. The importance of microRNAs in the cardiovascular system is now apparent. Mutations of distinct microRNAs have provided evidence for fundamental roles of microRNAs during cardiovascular development. However, there is limited information about global microRNA profiles during mouse heart development. In this study, we have gained insight from the expression profiles of microRNAs during mouse ventricular development by microarray and qRT-PCR analysis. Methods and results: Our microarray analysis reveals that relatively few microRNAs display either increasing or decreasing expression profiles during ventricular chamber formation. Interestingly, most of the differentially expressed microRNAs display a rather discrete peak of expression at particular developmental stages. Furthermore, we demonstrate that micro-RNA-27b (miR-27b) displays an overt myocardial expression during heart development and that the transcription factor-encoding gene Mef2c is an miR-27b target. Conclusion: Our data present a comprehensive profile of microRNA expression during ventricular maturation, providing an entry point for investigation of the functional roles of the most abundantly and differentially expressed microRNAs during cardiogenesis
publishDate 2010
dc.date.none.fl_str_mv 2010
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://academic.oup.com/cardiovascres/article/89/1/98/325183?login=true
https://hdl.handle.net/10953/4010
url https://academic.oup.com/cardiovascres/article/89/1/98/325183?login=true
https://hdl.handle.net/10953/4010
dc.relation.none.fl_str_mv Cardiovascular Research 2011, Vol 89, Issue 1
dc.rights.none.fl_str_mv http://creativecommons.org/publicdomain/zero/1.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/publicdomain/zero/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford
publisher.none.fl_str_mv Oxford
dc.source.none.fl_str_mv reponame:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
instname:Universidad de Jaén
instname_str Universidad de Jaén
reponame_str RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
collection RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
repository.name.fl_str_mv
repository.mail.fl_str_mv
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