Arsenic sensing and resistance system in the cyanobacterium Synechocystis sp. Strain PCC 6803

Arsenic is one of the most important global environmental pollutants. Here we show that the cyanobacterium Synechocystis sp. strain PCC 6803 contains an arsenic and antimony resistance operon consisting of three genes: arsB, encoding a putative arsenite and antimonite carrier, arsH, encoding a prote...

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Autores: López Maury, Luis, Florencio Bellido, Francisco Javier, Reyes Rosa, José Carlos
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2003
País:España
Recursos:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/62954
Acesso em linha:http://hdl.handle.net/11441/62954
https://doi.org/10.1128/JB.185.18.5363-5371.2003
Access Level:acceso abierto
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spelling Arsenic sensing and resistance system in the cyanobacterium Synechocystis sp. Strain PCC 6803López Maury, LuisFlorencio Bellido, Francisco JavierReyes Rosa, José CarlosArsenic is one of the most important global environmental pollutants. Here we show that the cyanobacterium Synechocystis sp. strain PCC 6803 contains an arsenic and antimony resistance operon consisting of three genes: arsB, encoding a putative arsenite and antimonite carrier, arsH, encoding a protein of unknown function, and arsC, encoding a putative arsenate reductase. While arsB mutant strains were sensitive to arsenite, arsenate, and antimonite, arsC mutants were sensitive only to arsenate. The arsH mutant strain showed no obvious phenotype under the conditions tested. In vivo the arsBHC operon was derepressed by oxyanions of arsenic and antimony (oxidation state, +3) and, to a lesser extent, by bismuth (oxidation state, +3) and arsenate (oxidation state, +5). In the absence of these effectors, the operon was repressed by a transcription repressor of the ArsR/SmtB family, encoded by an unlinked gene termed arsR. Thus, arsR null mutants showed constitutive derepression of the arsBHC operon. Expression of the arsR gene was not altered by the presence of arsenic or antimony compounds. Purified recombinant ArsR protein binds to the arsBHC promoter-operator region in the absence of metals and dissociates from the DNA in the presence of Sb(III) or As(III) but not in the presence of As(V), suggesting that trivalent metalloids are the true inducers of the system. DNase I footprinting experiments indicate that ArsR binds to two 17-bp direct repeats, with each one consisting of two inverted repeats, in the region from nucleotides -34 to + 17 of the arsBHC promoter-operator.Ministerio de Ciencia y Tecnología BMC2001-2635Junta de Andalucía CV1-802American Society for MicrobiologyBioquímica Vegetal y Biología MolecularMinisterio de Ciencia y Tecnología (MCYT). EspañaJunta de Andalucía2003info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/11441/62954https://doi.org/10.1128/JB.185.18.5363-5371.2003reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésJournal of Bacteriology, 185 (18), 5363-5371.BMC2001-2635CV1-802http://dx.doi.org/10.1128/JB.185.18.5363-5371.2003info:eu-repo/semantics/openAccessoai:idus.us.es:11441/629542026-06-17T12:51:07Z
dc.title.none.fl_str_mv Arsenic sensing and resistance system in the cyanobacterium Synechocystis sp. Strain PCC 6803
title Arsenic sensing and resistance system in the cyanobacterium Synechocystis sp. Strain PCC 6803
spellingShingle Arsenic sensing and resistance system in the cyanobacterium Synechocystis sp. Strain PCC 6803
López Maury, Luis
title_short Arsenic sensing and resistance system in the cyanobacterium Synechocystis sp. Strain PCC 6803
title_full Arsenic sensing and resistance system in the cyanobacterium Synechocystis sp. Strain PCC 6803
title_fullStr Arsenic sensing and resistance system in the cyanobacterium Synechocystis sp. Strain PCC 6803
title_full_unstemmed Arsenic sensing and resistance system in the cyanobacterium Synechocystis sp. Strain PCC 6803
title_sort Arsenic sensing and resistance system in the cyanobacterium Synechocystis sp. Strain PCC 6803
dc.creator.none.fl_str_mv López Maury, Luis
Florencio Bellido, Francisco Javier
Reyes Rosa, José Carlos
author López Maury, Luis
author_facet López Maury, Luis
Florencio Bellido, Francisco Javier
Reyes Rosa, José Carlos
author_role author
author2 Florencio Bellido, Francisco Javier
Reyes Rosa, José Carlos
author2_role author
author
dc.contributor.none.fl_str_mv Bioquímica Vegetal y Biología Molecular
Ministerio de Ciencia y Tecnología (MCYT). España
Junta de Andalucía
description Arsenic is one of the most important global environmental pollutants. Here we show that the cyanobacterium Synechocystis sp. strain PCC 6803 contains an arsenic and antimony resistance operon consisting of three genes: arsB, encoding a putative arsenite and antimonite carrier, arsH, encoding a protein of unknown function, and arsC, encoding a putative arsenate reductase. While arsB mutant strains were sensitive to arsenite, arsenate, and antimonite, arsC mutants were sensitive only to arsenate. The arsH mutant strain showed no obvious phenotype under the conditions tested. In vivo the arsBHC operon was derepressed by oxyanions of arsenic and antimony (oxidation state, +3) and, to a lesser extent, by bismuth (oxidation state, +3) and arsenate (oxidation state, +5). In the absence of these effectors, the operon was repressed by a transcription repressor of the ArsR/SmtB family, encoded by an unlinked gene termed arsR. Thus, arsR null mutants showed constitutive derepression of the arsBHC operon. Expression of the arsR gene was not altered by the presence of arsenic or antimony compounds. Purified recombinant ArsR protein binds to the arsBHC promoter-operator region in the absence of metals and dissociates from the DNA in the presence of Sb(III) or As(III) but not in the presence of As(V), suggesting that trivalent metalloids are the true inducers of the system. DNase I footprinting experiments indicate that ArsR binds to two 17-bp direct repeats, with each one consisting of two inverted repeats, in the region from nucleotides -34 to + 17 of the arsBHC promoter-operator.
publishDate 2003
dc.date.none.fl_str_mv 2003
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11441/62954
https://doi.org/10.1128/JB.185.18.5363-5371.2003
url http://hdl.handle.net/11441/62954
https://doi.org/10.1128/JB.185.18.5363-5371.2003
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of Bacteriology, 185 (18), 5363-5371.
BMC2001-2635
CV1-802
http://dx.doi.org/10.1128/JB.185.18.5363-5371.2003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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