Production of biologically active Cecropin A peptide in rice seed oil bodies

Cecropin A is a natural antimicrobial peptide that exhibits fast and potent activity against a broad spectrum of pathogens and neoplastic cells, and that has important biotechnological applications. However, cecropin A exploitation, as for other antimicrobial peptides, is limited by their production...

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Autores: Montesinos Barreda, Laura, Bundó, Mireia, Izquierdo, Esther, Campo, Sonia, Badosa Romañó, Esther, Rossignol, Michel, Montesinos Seguí, Emilio, San Segundo de los Mozos, Blanca, Coca, María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/11910
Acceso en línea:http://hdl.handle.net/10256/11910
Access Level:acceso abierto
Palabra clave:Pèptids
Plantes transgèniques
Arròs
Peptides
Transgenic plants
Rice
Arròs -- Protecció
Rice -- Protection
Llavors -- Protecció
Seeds -- Protection
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network_name_str España
repository_id_str
spelling Production of biologically active Cecropin A peptide in rice seed oil bodiesMontesinos Barreda, LauraBundó, MireiaIzquierdo, EstherCampo, SoniaBadosa Romañó, EstherRossignol, MichelMontesinos Seguí, EmilioSan Segundo de los Mozos, BlancaCoca, MaríaPèptidsPlantes transgèniquesArròsPeptidesTransgenic plantsRiceArròs -- ProteccióRice -- ProtectionLlavors -- ProteccióSeeds -- ProtectionCecropin A is a natural antimicrobial peptide that exhibits fast and potent activity against a broad spectrum of pathogens and neoplastic cells, and that has important biotechnological applications. However, cecropin A exploitation, as for other antimicrobial peptides, is limited by their production and purification costs. Here, we report the efficient production of this bioactive peptide in rice bran using the rice oleosin 18 as a carrier protein. High cecropin A levels were reached in rice seeds driving the expression of the chimeric gene by the strong embryo-specific oleosin 18 own promoter, and targeting the peptide to the oil body organelle as an oleosin 18-cecropin A fusion protein. The accumulation of cecropin A in oil bodies had no deleterious effects on seed viability and seedling growth, as well as on seed yield. We also show that biologically active cecropin A can be easily purified from the transgenic rice seeds by homogenization and simple flotation centrifugation methods. Our results demonstrate that the oleosin fusion technology is suitable for the production of cecropin A in rice seeds, which can potentially be extended to other antimicrobial peptides to assist their exploitationThis work was supported by the Spanish Ministry of Economy and Competitiveness [Grants EUI2008-03769, EUI2008-03572 and BIO2012- 32838], and by the French Research Agency [Grant ANR-08-KBBE-010]Public Library of Science (PLoS)Ministerio de Educación y Ciencia (Espanya)2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion18 p.application/pdfhttp://hdl.handle.net/10256/11910http://hdl.handle.net/10256/11910PLoS One, 2016, vol. 11, num. 1, p. e0146919-e0146937Montesinos Barreda, Laura Bundó, Mireia Izquierdo, Esther Campo, Sonia Badosa Romañó, Esther Rossignol, Michel Montesinos Seguí, Emilio San Segundo de los Mozos, Blanca Coca, María 2016 Production of biologically active Cecropin A peptide in rice seed oil bodies PLoS One 11 1 e0146919 e0146937reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0146919info:eu-repo/semantics/altIdentifier/eissn/1932-6203info:eu-repo/semantics/altIdentifier/eissn/1932-6203info:eu-repo/grantAgreement/MICINN//EUI2008-03572Reconeixement 3.0 Espanyahttp://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:10256/119102026-05-29T05:05:01Z
dc.title.none.fl_str_mv Production of biologically active Cecropin A peptide in rice seed oil bodies
title Production of biologically active Cecropin A peptide in rice seed oil bodies
spellingShingle Production of biologically active Cecropin A peptide in rice seed oil bodies
Montesinos Barreda, Laura
Pèptids
Plantes transgèniques
Arròs
Peptides
Transgenic plants
Rice
Arròs -- Protecció
Rice -- Protection
Llavors -- Protecció
Seeds -- Protection
title_short Production of biologically active Cecropin A peptide in rice seed oil bodies
title_full Production of biologically active Cecropin A peptide in rice seed oil bodies
title_fullStr Production of biologically active Cecropin A peptide in rice seed oil bodies
title_full_unstemmed Production of biologically active Cecropin A peptide in rice seed oil bodies
title_sort Production of biologically active Cecropin A peptide in rice seed oil bodies
dc.creator.none.fl_str_mv Montesinos Barreda, Laura
Bundó, Mireia
Izquierdo, Esther
Campo, Sonia
Badosa Romañó, Esther
Rossignol, Michel
Montesinos Seguí, Emilio
San Segundo de los Mozos, Blanca
Coca, María
author Montesinos Barreda, Laura
author_facet Montesinos Barreda, Laura
Bundó, Mireia
Izquierdo, Esther
Campo, Sonia
Badosa Romañó, Esther
Rossignol, Michel
Montesinos Seguí, Emilio
San Segundo de los Mozos, Blanca
Coca, María
author_role author
author2 Bundó, Mireia
Izquierdo, Esther
Campo, Sonia
Badosa Romañó, Esther
Rossignol, Michel
Montesinos Seguí, Emilio
San Segundo de los Mozos, Blanca
Coca, María
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Educación y Ciencia (Espanya)
dc.subject.none.fl_str_mv Pèptids
Plantes transgèniques
Arròs
Peptides
Transgenic plants
Rice
Arròs -- Protecció
Rice -- Protection
Llavors -- Protecció
Seeds -- Protection
topic Pèptids
Plantes transgèniques
Arròs
Peptides
Transgenic plants
Rice
Arròs -- Protecció
Rice -- Protection
Llavors -- Protecció
Seeds -- Protection
description Cecropin A is a natural antimicrobial peptide that exhibits fast and potent activity against a broad spectrum of pathogens and neoplastic cells, and that has important biotechnological applications. However, cecropin A exploitation, as for other antimicrobial peptides, is limited by their production and purification costs. Here, we report the efficient production of this bioactive peptide in rice bran using the rice oleosin 18 as a carrier protein. High cecropin A levels were reached in rice seeds driving the expression of the chimeric gene by the strong embryo-specific oleosin 18 own promoter, and targeting the peptide to the oil body organelle as an oleosin 18-cecropin A fusion protein. The accumulation of cecropin A in oil bodies had no deleterious effects on seed viability and seedling growth, as well as on seed yield. We also show that biologically active cecropin A can be easily purified from the transgenic rice seeds by homogenization and simple flotation centrifugation methods. Our results demonstrate that the oleosin fusion technology is suitable for the production of cecropin A in rice seeds, which can potentially be extended to other antimicrobial peptides to assist their exploitation
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/11910
http://hdl.handle.net/10256/11910
url http://hdl.handle.net/10256/11910
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0146919
info:eu-repo/semantics/altIdentifier/eissn/1932-6203
info:eu-repo/semantics/altIdentifier/eissn/1932-6203
info:eu-repo/grantAgreement/MICINN//EUI2008-03572
dc.rights.none.fl_str_mv Reconeixement 3.0 Espanya
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Reconeixement 3.0 Espanya
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 18 p.
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv PLoS One, 2016, vol. 11, num. 1, p. e0146919-e0146937
Montesinos Barreda, Laura Bundó, Mireia Izquierdo, Esther Campo, Sonia Badosa Romañó, Esther Rossignol, Michel Montesinos Seguí, Emilio San Segundo de los Mozos, Blanca Coca, María 2016 Production of biologically active Cecropin A peptide in rice seed oil bodies PLoS One 11 1 e0146919 e0146937
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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