Anti-trbc1 antibody-based flow cytometric detection of t-cell clonality: Standardization of sample preparation and diagnostic implementation

© 2021 by the authors.

Detalles Bibliográficos
Autores: Muñoz-García, Noemí, Lima, Margarida, Villamor, Neus, Morán-Plata, F. Javier, Barrena, Susana, Mateos, Sheila, Caldas, Carolina, Balanzategui, Ana, Alcoceba, Miguel, Domínguez, Alejandro, Gómez, Fabio, Langerak, Anton W., Dongen, J. J. M. van, Orfao, Alberto, Almeida, Julia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/260999
Acceso en línea:http://hdl.handle.net/10261/260999
Access Level:acceso abierto
Palabra clave:TRBC1
JOVI-1
T-CLPD
Tαβ-cells
TRBJ1 and TRBJ2
TCRVβ
MRD1
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spelling Anti-trbc1 antibody-based flow cytometric detection of t-cell clonality: Standardization of sample preparation and diagnostic implementationMuñoz-García, NoemíLima, MargaridaVillamor, NeusMorán-Plata, F. JavierBarrena, SusanaMateos, SheilaCaldas, CarolinaBalanzategui, AnaAlcoceba, MiguelDomínguez, AlejandroGómez, FabioLangerak, Anton W.Dongen, J. J. M. vanOrfao, AlbertoAlmeida, JuliaTRBC1JOVI-1T-CLPDTαβ-cellsTRBJ1 and TRBJ2TCRVβMRD1© 2021 by the authors.A single antibody (anti-TRBC1; JOVI-1 antibody clone) against one of the two mutually exclusive T-cell receptor β-chain constant domains was identified as a potentially useful flow-cytometry (FCM) marker to assess Tαβ-cell clonality. We optimized the TRBC1-FCM approach for detecting clonal Tαβ-cells and validated the method in 211 normal, reactive and pathological samples. TRBC1 labeling significantly improved in the presence of CD3. Purified TRBC1+ and TRBC1− monoclonal and polyclonal Tαβ-cells rearranged TRBJ1 in 44/47 (94%) and TRBJ1+TRBJ2 in 48 of 48 (100%) populations, respectively, which confirmed the high specificity of this assay. Additionally, TRBC1+/TRBC1− ratios within different Tαβ-cell subsets are provided as reference for polyclonal cells, among which a bimodal pattern of TRBC1-expression profile was found for all TCRVβ families, whereas highly-variable TRBC1+/TRBC1− ratios were observed in more mature vs. naïve Tαβ-cell subsets (vs. total T-cells). In 112/117 (96%) samples containing clonal Tαβ-cells in which the approach was validated, monotypic expression of TRBC1 was confirmed. Dilutional experiments showed a level of detection for detecting clonal Tαβ-cells of ≤10−4 in seven out of eight pathological samples. These results support implementation of the optimized TRBC1-FCM approach as a fast, specific and accurate method for assessing T-cell clonality in diagnostic-FCM panels, and for minimal (residual) disease detection in mature Tαβ+ leukemia/lymphoma patients.This work was supported by the CB16/12/00400 (CIBERONC) and PI20-01346 grants, from the Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación (Madrid, Spain) and FONDOS FEDER, and by the EuroFlow Foundation (Leiden, The Netherlands). N. Muñoz-García is supported by a pre-doctoral grant (Ref. IBPredoc17/00012) from IBSAL (Salamanca, Spain). M. Lima, N. Villamor, A.W. Langerak, J.J.M. van Dongen, A. Orfao, and J. Almeida are members of the EuroFlow ConsortiumMultidisciplinary Digital Publishing InstituteInstituto de Salud Carlos IIIMinisterio de Ciencia e Innovación (España)European CommissionInstituto de Investigación Biomédica de SalamancaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220212022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/260999reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3390/cancers13174379Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2609992026-05-22T06:33:51Z
dc.title.none.fl_str_mv Anti-trbc1 antibody-based flow cytometric detection of t-cell clonality: Standardization of sample preparation and diagnostic implementation
title Anti-trbc1 antibody-based flow cytometric detection of t-cell clonality: Standardization of sample preparation and diagnostic implementation
spellingShingle Anti-trbc1 antibody-based flow cytometric detection of t-cell clonality: Standardization of sample preparation and diagnostic implementation
Muñoz-García, Noemí
TRBC1
JOVI-1
T-CLPD
Tαβ-cells
TRBJ1 and TRBJ2
TCRVβ
MRD1
title_short Anti-trbc1 antibody-based flow cytometric detection of t-cell clonality: Standardization of sample preparation and diagnostic implementation
title_full Anti-trbc1 antibody-based flow cytometric detection of t-cell clonality: Standardization of sample preparation and diagnostic implementation
title_fullStr Anti-trbc1 antibody-based flow cytometric detection of t-cell clonality: Standardization of sample preparation and diagnostic implementation
title_full_unstemmed Anti-trbc1 antibody-based flow cytometric detection of t-cell clonality: Standardization of sample preparation and diagnostic implementation
title_sort Anti-trbc1 antibody-based flow cytometric detection of t-cell clonality: Standardization of sample preparation and diagnostic implementation
dc.creator.none.fl_str_mv Muñoz-García, Noemí
Lima, Margarida
Villamor, Neus
Morán-Plata, F. Javier
Barrena, Susana
Mateos, Sheila
Caldas, Carolina
Balanzategui, Ana
Alcoceba, Miguel
Domínguez, Alejandro
Gómez, Fabio
Langerak, Anton W.
Dongen, J. J. M. van
Orfao, Alberto
Almeida, Julia
author Muñoz-García, Noemí
author_facet Muñoz-García, Noemí
Lima, Margarida
Villamor, Neus
Morán-Plata, F. Javier
Barrena, Susana
Mateos, Sheila
Caldas, Carolina
Balanzategui, Ana
Alcoceba, Miguel
Domínguez, Alejandro
Gómez, Fabio
Langerak, Anton W.
Dongen, J. J. M. van
Orfao, Alberto
Almeida, Julia
author_role author
author2 Lima, Margarida
Villamor, Neus
Morán-Plata, F. Javier
Barrena, Susana
Mateos, Sheila
Caldas, Carolina
Balanzategui, Ana
Alcoceba, Miguel
Domínguez, Alejandro
Gómez, Fabio
Langerak, Anton W.
Dongen, J. J. M. van
Orfao, Alberto
Almeida, Julia
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
Ministerio de Ciencia e Innovación (España)
European Commission
Instituto de Investigación Biomédica de Salamanca
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv TRBC1
JOVI-1
T-CLPD
Tαβ-cells
TRBJ1 and TRBJ2
TCRVβ
MRD1
topic TRBC1
JOVI-1
T-CLPD
Tαβ-cells
TRBJ1 and TRBJ2
TCRVβ
MRD1
description © 2021 by the authors.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/260999
url http://hdl.handle.net/10261/260999
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3390/cancers13174379
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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