Crosstalk between epigenetic silencing and infection by tobacco rattle virus in Arabidopsis

14 p.-6 fig.

Detalhes bibliográficos
Autores: Diezma-Navas, Laura, Pérez-González, Ana, Artaza-Álvarez, Haydeé, Alonso, Lola, Caro, Elena, Llave, César, Ruiz-Ferrer, Virginia
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/185748
Acesso em linha:http://hdl.handle.net/10261/185748
Access Level:acceso abierto
Palavra-chave:Arabidopsis thaliana
Disease resistance genes
DNA methylation
Plant–virus interactions
Plant viruses
RNA-dependent DNA methylation
Tobacco rattle virus
Transposable elements
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spelling Crosstalk between epigenetic silencing and infection by tobacco rattle virus in ArabidopsisDiezma-Navas, LauraPérez-González, AnaArtaza-Álvarez, HaydeéAlonso, LolaCaro, ElenaLlave, CésarRuiz-Ferrer, VirginiaArabidopsis thalianaDisease resistance genesDNA methylationPlant–virus interactionsPlant virusesRNA-dependent DNA methylationTobacco rattle virusTransposable elements14 p.-6 fig.DNA methylation is an important epigenetic mechanism for controlling innate immunity against microbial pathogens in plants. Little is known, however, about the manner in which viral infections interact with DNA methylation pathways. Here we investigate the crosstalk between epigenetic silencing and viral infections in Arabidopsis inflorescences. We found that tobacco rattle virus (TRV) causes changes in the expression of key transcriptional gene silencing factors with RNA‐directed DNA methylation activities that coincide with changes in methylation at the whole genome level. Viral susceptibility/resistance was altered in DNA (de)methylation‐deficient mutants, suggesting that DNA methylation is an important regulatory system controlling TRV proliferation. We further show that several transposable elements (TEs) underwent transcriptional activation during TRV infection, and that TE regulation likely involved both DNA methylation‐dependent and ‐independent mechanisms. We identified a cluster of disease resistance genes regulated by DNA methylation in infected plants that were enriched for TEs in their promoters. Interestingly, TEs and nearby resistance genes were co‐regulated in TRV‐infected DNA (de)methylation mutants. Our study shows that DNA methylation contributes to modulate the outcome of viral infections in Arabidopsis, and opens up new possibilities for exploring the role of TE regulation in antiviral defence.This work has been supported by a Ramon y Cajal grant (RyC‐2011‐07006) to VRF and by National Research grants BIO2012‐39973 and BIO2015‐70752‐R to CL from Ministerio de Economía y Competitividad (MINECO/FEDER), Spain.Peer reviewedJohn Wiley & SonsMinisterio de Economía y Competitividad (España)Diezma-Navas, Laura [0000-0001-5857-5091]Pérez-González, Ana [0000-0002-7724-9985]Artaza-Álvarez, Haydeé [0000-0002-1585-5488]Alonso, Lola [0000-0002-3493-718X]Caro, Elena [0000-0002-1034-1621]Llave, César [0000-0003-3844-4582]Ruiz-Ferrer, Virginia [0000-0002-7840-297X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/185748reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2015-70752-Rhttps://doi.org/10.1111/mpp.12850Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1857482026-05-22T06:33:51Z
dc.title.none.fl_str_mv Crosstalk between epigenetic silencing and infection by tobacco rattle virus in Arabidopsis
title Crosstalk between epigenetic silencing and infection by tobacco rattle virus in Arabidopsis
spellingShingle Crosstalk between epigenetic silencing and infection by tobacco rattle virus in Arabidopsis
Diezma-Navas, Laura
Arabidopsis thaliana
Disease resistance genes
DNA methylation
Plant–virus interactions
Plant viruses
RNA-dependent DNA methylation
Tobacco rattle virus
Transposable elements
title_short Crosstalk between epigenetic silencing and infection by tobacco rattle virus in Arabidopsis
title_full Crosstalk between epigenetic silencing and infection by tobacco rattle virus in Arabidopsis
title_fullStr Crosstalk between epigenetic silencing and infection by tobacco rattle virus in Arabidopsis
title_full_unstemmed Crosstalk between epigenetic silencing and infection by tobacco rattle virus in Arabidopsis
title_sort Crosstalk between epigenetic silencing and infection by tobacco rattle virus in Arabidopsis
dc.creator.none.fl_str_mv Diezma-Navas, Laura
Pérez-González, Ana
Artaza-Álvarez, Haydeé
Alonso, Lola
Caro, Elena
Llave, César
Ruiz-Ferrer, Virginia
author Diezma-Navas, Laura
author_facet Diezma-Navas, Laura
Pérez-González, Ana
Artaza-Álvarez, Haydeé
Alonso, Lola
Caro, Elena
Llave, César
Ruiz-Ferrer, Virginia
author_role author
author2 Pérez-González, Ana
Artaza-Álvarez, Haydeé
Alonso, Lola
Caro, Elena
Llave, César
Ruiz-Ferrer, Virginia
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Diezma-Navas, Laura [0000-0001-5857-5091]
Pérez-González, Ana [0000-0002-7724-9985]
Artaza-Álvarez, Haydeé [0000-0002-1585-5488]
Alonso, Lola [0000-0002-3493-718X]
Caro, Elena [0000-0002-1034-1621]
Llave, César [0000-0003-3844-4582]
Ruiz-Ferrer, Virginia [0000-0002-7840-297X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Arabidopsis thaliana
Disease resistance genes
DNA methylation
Plant–virus interactions
Plant viruses
RNA-dependent DNA methylation
Tobacco rattle virus
Transposable elements
topic Arabidopsis thaliana
Disease resistance genes
DNA methylation
Plant–virus interactions
Plant viruses
RNA-dependent DNA methylation
Tobacco rattle virus
Transposable elements
description 14 p.-6 fig.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/185748
url http://hdl.handle.net/10261/185748
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2015-70752-R
https://doi.org/10.1111/mpp.12850
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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