The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanisms

The Lsm1-7-Pat1 complex binds to the 3' end of cellular mRNAs and promotes 3' end protection and 5'-3' decay. Interestingly, this complex also specifically binds to cis-acting regulatory sequences of viral positive-strand RNA genomes promoting their translation an...

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Detalles Bibliográficos
Autores: Jungfleisch, Jennifer, 1986-, Chowdhury, Ashis, Alves-Rodrigues, Isabel, Tharun, Sundaresan, Díez Antón, Juana, 1962-
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Universitat Pompeu Fabra
Repositorio:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/25729
Acceso en línea:http://hdl.handle.net/10230/25729
http://dx.doi.org/10.1261/rna.052209.115
Access Level:acceso abierto
Palabra clave:Saccharomyces cerevisiae -- Metabolisme
RNA missatger
Lsm1-7
Pat1
Lsm1-7-Pat1 complex
Decapping activators
Viral RNA
BMV
RNA virus
Lsm proteins
Translation
Replication
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repository_id_str
spelling The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanismsJungfleisch, Jennifer, 1986-Chowdhury, AshisAlves-Rodrigues, IsabelTharun, SundaresanDíez Antón, Juana, 1962-Saccharomyces cerevisiae -- MetabolismeRNA missatgerLsm1-7Pat1Lsm1-7-Pat1 complexDecapping activatorsViral RNABMVRNA virusLsm proteinsTranslationReplicationThe Lsm1-7-Pat1 complex binds to the 3' end of cellular mRNAs and promotes 3' end protection and 5'-3' decay. Interestingly, this complex also specifically binds to cis-acting regulatory sequences of viral positive-strand RNA genomes promoting their translation and subsequent recruitment from translation to replication. Yet, how the Lsm1-7-Pat1 complex regulates these two processes remains elusive. Here, we show that Lsm1-7-Pat1 complex acts differentially in these processes. By using a collection of well-characterized lsm1 mutant alleles and a system that allows the replication of Brome mosaic virus (BMV) in yeast we show that the Lsm1-7-Pat1 complex integrity is essential for both, translation and recruitment. However, the intrinsic RNA-binding ability of the complex is only required for translation. Consistent with an RNA-binding-independent function of the Lsm1-7-Pat1 complex on BMV RNA recruitment, we show that the BMV 1a protein, the sole viral protein required for recruitment, interacts with this complex in an RNA-independent manner. Together, these results support a model wherein Lsm1-7-Pat1 complex binds consecutively to BMV RNA regulatory sequences and the 1a protein to promote viral RNA translation and later recruitment out of the host translation machinery to the viral replication complexes.This work was supported by a grant from the Spanish Ministerio de Ciencia e Innovación (BFU2013-44629-R) and USUHS intramural grant to S.T. J.J. was supported by grant 2012FI_B00574 from the Generalitat de Catalunya.Cold Spring Harbor Laboratory Press201620162015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/25729http://dx.doi.org/10.1261/rna.052209.115reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésRNA. 2015;21(8):1469-79info:eu-repo/grantAgreement/ES/1PE/BFU2013-44629-R© 2015, RNA by RNA Society. Published version available at http://rnajournal.cshlp.org/content/21/8/1469.longhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/257292026-06-12T07:21:37Z
dc.title.none.fl_str_mv The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanisms
title The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanisms
spellingShingle The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanisms
Jungfleisch, Jennifer, 1986-
Saccharomyces cerevisiae -- Metabolisme
RNA missatger
Lsm1-7
Pat1
Lsm1-7-Pat1 complex
Decapping activators
Viral RNA
BMV
RNA virus
Lsm proteins
Translation
Replication
title_short The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanisms
title_full The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanisms
title_fullStr The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanisms
title_full_unstemmed The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanisms
title_sort The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanisms
dc.creator.none.fl_str_mv Jungfleisch, Jennifer, 1986-
Chowdhury, Ashis
Alves-Rodrigues, Isabel
Tharun, Sundaresan
Díez Antón, Juana, 1962-
author Jungfleisch, Jennifer, 1986-
author_facet Jungfleisch, Jennifer, 1986-
Chowdhury, Ashis
Alves-Rodrigues, Isabel
Tharun, Sundaresan
Díez Antón, Juana, 1962-
author_role author
author2 Chowdhury, Ashis
Alves-Rodrigues, Isabel
Tharun, Sundaresan
Díez Antón, Juana, 1962-
author2_role author
author
author
author
dc.subject.none.fl_str_mv Saccharomyces cerevisiae -- Metabolisme
RNA missatger
Lsm1-7
Pat1
Lsm1-7-Pat1 complex
Decapping activators
Viral RNA
BMV
RNA virus
Lsm proteins
Translation
Replication
topic Saccharomyces cerevisiae -- Metabolisme
RNA missatger
Lsm1-7
Pat1
Lsm1-7-Pat1 complex
Decapping activators
Viral RNA
BMV
RNA virus
Lsm proteins
Translation
Replication
description The Lsm1-7-Pat1 complex binds to the 3' end of cellular mRNAs and promotes 3' end protection and 5'-3' decay. Interestingly, this complex also specifically binds to cis-acting regulatory sequences of viral positive-strand RNA genomes promoting their translation and subsequent recruitment from translation to replication. Yet, how the Lsm1-7-Pat1 complex regulates these two processes remains elusive. Here, we show that Lsm1-7-Pat1 complex acts differentially in these processes. By using a collection of well-characterized lsm1 mutant alleles and a system that allows the replication of Brome mosaic virus (BMV) in yeast we show that the Lsm1-7-Pat1 complex integrity is essential for both, translation and recruitment. However, the intrinsic RNA-binding ability of the complex is only required for translation. Consistent with an RNA-binding-independent function of the Lsm1-7-Pat1 complex on BMV RNA recruitment, we show that the BMV 1a protein, the sole viral protein required for recruitment, interacts with this complex in an RNA-independent manner. Together, these results support a model wherein Lsm1-7-Pat1 complex binds consecutively to BMV RNA regulatory sequences and the 1a protein to promote viral RNA translation and later recruitment out of the host translation machinery to the viral replication complexes.
publishDate 2015
dc.date.none.fl_str_mv 2015
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/25729
http://dx.doi.org/10.1261/rna.052209.115
url http://hdl.handle.net/10230/25729
http://dx.doi.org/10.1261/rna.052209.115
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv RNA. 2015;21(8):1469-79
info:eu-repo/grantAgreement/ES/1PE/BFU2013-44629-R
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
collection Repositorio Digital de la UPF
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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