Assessment of twelve echovirus virus-neutralisation assays in Europe: recommendations for harmonisation of non-polio enterovirus sero-surveillance studies

Non-polio enteroviruses (NPEV) cause significant disease worldwide. Population-based sero-surveillance, by measuring antibodies against specific NPEV types, provides additional information on past circulation and the prediction for future upsurges. Virus neutralisation assays (VNA), the current meth...

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Detalles Bibliográficos
Autores: Couderé, Karen, Benschop, Kimberley SM, Koen, Gerrit, van Eijk, Hetty, Harvala, Heli, Bailly, Jean-Luc, Bessaud, Maël, Kamau, Everlyn, Simon, Isabelle, Joffret, Marie-Line, Nikolaeva-Glomb, Lubomira, Georgieva, Irina, Stoyanova, Asya, Diedrich, Sabine, Böttcher, Sindy, Cabrerizo, Maria, Tabain, Irena, Hruškar, Željka, Stevanović, Vladimir, Susi, Petri, Hietanen, Eero, Palminha, Paula, Rainetová, Petra, Baicus, Anda, Kristensen, Maartje, PajkrtJean-Luc Murk, Dasja, Wolthers, Katja C, European Non-Polio Enterovirus Network
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/26575
Acceso en línea:https://hdl.handle.net/20.500.12105/26575
Access Level:acceso abierto
Palabra clave:Virus neutralisation assay
Echovirus
Enterovirus
Standardisation
Surveillance
Antibodies, Neutralizing
Antibodies, Viral
Echovirus Infections
Enterovirus B, Human
Enterovirus Infections
Europe
Humans
Neutralization Tests
Seroepidemiologic Studies
Descripción
Sumario:Non-polio enteroviruses (NPEV) cause significant disease worldwide. Population-based sero-surveillance, by measuring antibodies against specific NPEV types, provides additional information on past circulation and the prediction for future upsurges. Virus neutralisation assays (VNA), the current method of choice for measuring NPEV type specific antibodies, are not entirely standardised. Via the European Non-Polio Enterovirus Network, we organised a VNA quality assessment in which twelve laboratories participated. We provided five echovirus (E) types (E1, E18, E30 G2, E30 G6 and E6) and intravenous immunoglobulins (IVIG) as a sample for the NPEV VNA quality assessment. Differences in VNA protocols and neutralising Ab (nAb) titres were found between the participating laboratories with geometric coefficients of variation ranging from 10.3-62.9 %. Mixed-effects regression analysis indicated a small but significant effect of type of cell line used. Harmonisation of cell line passage number, however, did not improve variation between laboratories. Calibration by making use of a reference sample, reduced variation between laboratories but differences in nAb titres remained higher than two log2 dilution steps. In conclusion, sero-surveillance data from different laboratories should be compared with caution and standardised protocols are needed.

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