2D NMR study of lignin modification during pretreatment of Eucalyptus wood feedstock with a fungal laccase and a phenolic mediator

The delignification of eucalypt feedstock during enzymatic pretreatment with the laccase from Myceliophthora thermophila, in conjunction with methyl syringate as mediator, when applied in a multistage sequence consisting of successive enzymatic and alkaline extraction stages, directly on the ground...

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Autores: Rico Campos, Alejandro, Rencoret, Jorge, Río Andrade, José Carlos del, Martínez, Ángel T., Gutiérrez Suárez, Ana
Tipo de recurso: otro
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/98720
Acceso en línea:http://hdl.handle.net/10261/98720
Access Level:acceso abierto
Palabra clave:Eucalyptus wood
Delignification
Enzymatic pretreatment
Laccases
Lignin
2D NMR
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spelling 2D NMR study of lignin modification during pretreatment of Eucalyptus wood feedstock with a fungal laccase and a phenolic mediatorRico Campos, AlejandroRencoret, JorgeRío Andrade, José Carlos delMartínez, Ángel T.Gutiérrez Suárez, AnaEucalyptus woodDelignificationEnzymatic pretreatmentLaccasesLignin2D NMRThe delignification of eucalypt feedstock during enzymatic pretreatment with the laccase from Myceliophthora thermophila, in conjunction with methyl syringate as mediator, when applied in a multistage sequence consisting of successive enzymatic and alkaline extraction stages, directly on the ground lignocellulosic material was thoroughly studied by two-dimensional nuclear magnetic resonance (2D NMR) of the whole wood (at the gel stage) after each step of the sequence. The 2D NMR analyses of eucalypt feedstock after the enzymatic pretreatment, revealed the removal of guaiacyl and syringyl lignin units (with preferential removal of the former) and aliphatic (mainly beta-O-4'-linked) side-chains of lignin without a substantial change in polysaccharide crosssignals. However, the most noticeable modification was the formation of Calpha-oxidized syringyl lignin units in the enzymatic steps that were partially removed in the alkaline extraction stages. This study revealed that the first cycle of pretreatment of eucalypt with M. thermophila laccase and methyl syringate did not produce any delignification effect and consequently did not increase the saccharification yields. However, at the end of the whole sequence (4 cycles), a high delignification (ca. 50%) of eucalypt feedstock and increases (ca. 40%) in glucose yield after enzymatic hydrolysis was attained with this laccase-mediator pretreatment.This study was funded by the INDOX (KBBE-2013-7-613549) EU-project and the LIGNOCELL (AGL2011-25379) Spanish MICINN project (co-financed by FEDER funds). J. Rencoret acknowledges the CSIC JAE-Doc contract of the program “Junta para la Ampliación de Estudios” co-financed by the European Social Fund (ESF). H. Lund and L. Kalum from Novozymes (Bagsvaerd, Denmark) are acknowledged for the cellulases and the M. thermophila laccase and J. Romero (ENCE, Spain) for the eucalypt wood samples. Manuel Angulo (CITIUS, University of Seville) is acknowledged for providing technical assistance in the NMR analyses.Peer reviewedCSIC - Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201420142014info:eu-repo/semantics/otherhttp://purl.org/coar/resource_type/c_3248info:eu-repo/semantics/bookParthttp://hdl.handle.net/10261/98720reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#KBBE-2013-7-613549Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/987202026-05-22T06:33:51Z
dc.title.none.fl_str_mv 2D NMR study of lignin modification during pretreatment of Eucalyptus wood feedstock with a fungal laccase and a phenolic mediator
title 2D NMR study of lignin modification during pretreatment of Eucalyptus wood feedstock with a fungal laccase and a phenolic mediator
spellingShingle 2D NMR study of lignin modification during pretreatment of Eucalyptus wood feedstock with a fungal laccase and a phenolic mediator
Rico Campos, Alejandro
Eucalyptus wood
Delignification
Enzymatic pretreatment
Laccases
Lignin
2D NMR
title_short 2D NMR study of lignin modification during pretreatment of Eucalyptus wood feedstock with a fungal laccase and a phenolic mediator
title_full 2D NMR study of lignin modification during pretreatment of Eucalyptus wood feedstock with a fungal laccase and a phenolic mediator
title_fullStr 2D NMR study of lignin modification during pretreatment of Eucalyptus wood feedstock with a fungal laccase and a phenolic mediator
title_full_unstemmed 2D NMR study of lignin modification during pretreatment of Eucalyptus wood feedstock with a fungal laccase and a phenolic mediator
title_sort 2D NMR study of lignin modification during pretreatment of Eucalyptus wood feedstock with a fungal laccase and a phenolic mediator
dc.creator.none.fl_str_mv Rico Campos, Alejandro
Rencoret, Jorge
Río Andrade, José Carlos del
Martínez, Ángel T.
Gutiérrez Suárez, Ana
author Rico Campos, Alejandro
author_facet Rico Campos, Alejandro
Rencoret, Jorge
Río Andrade, José Carlos del
Martínez, Ángel T.
Gutiérrez Suárez, Ana
author_role author
author2 Rencoret, Jorge
Río Andrade, José Carlos del
Martínez, Ángel T.
Gutiérrez Suárez, Ana
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Eucalyptus wood
Delignification
Enzymatic pretreatment
Laccases
Lignin
2D NMR
topic Eucalyptus wood
Delignification
Enzymatic pretreatment
Laccases
Lignin
2D NMR
description The delignification of eucalypt feedstock during enzymatic pretreatment with the laccase from Myceliophthora thermophila, in conjunction with methyl syringate as mediator, when applied in a multistage sequence consisting of successive enzymatic and alkaline extraction stages, directly on the ground lignocellulosic material was thoroughly studied by two-dimensional nuclear magnetic resonance (2D NMR) of the whole wood (at the gel stage) after each step of the sequence. The 2D NMR analyses of eucalypt feedstock after the enzymatic pretreatment, revealed the removal of guaiacyl and syringyl lignin units (with preferential removal of the former) and aliphatic (mainly beta-O-4'-linked) side-chains of lignin without a substantial change in polysaccharide crosssignals. However, the most noticeable modification was the formation of Calpha-oxidized syringyl lignin units in the enzymatic steps that were partially removed in the alkaline extraction stages. This study revealed that the first cycle of pretreatment of eucalypt with M. thermophila laccase and methyl syringate did not produce any delignification effect and consequently did not increase the saccharification yields. However, at the end of the whole sequence (4 cycles), a high delignification (ca. 50%) of eucalypt feedstock and increases (ca. 40%) in glucose yield after enzymatic hydrolysis was attained with this laccase-mediator pretreatment.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/other
http://purl.org/coar/resource_type/c_3248
dc.type.openaire.fl_str_mv info:eu-repo/semantics/bookPart
format other
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/98720
url http://hdl.handle.net/10261/98720
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
KBBE-2013-7-613549
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv CSIC - Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS)
publisher.none.fl_str_mv CSIC - Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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