Application of ultrasound technique to evaluate the testicular function and its correlation to the sperm quality after different collection frequency in rams

[EN] The frequency of semen collection is a crucial factor to consider in the rams performance inside breeding centers workout. To evaluate this factor, ram Breeding Soundness Evaluation could include sperm quality evaluation and new predictive and non-invasive tools such as ultrasound technique. In...

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Autores: Montes Garrido, Rafael, Fernández Riesco, Marta, Anel López, Luis, Neila Montero, Marta, Palacín Martínez, Cristina, Boixo, Juan Carlos, Paz Cabello, Paulino de, Ortega Ferrusola, Cristina, Hassan, Mohamed A. A., Anel Rodríguez, Luis, Álvarez García, Mercedes
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad Rey Juan Carlos
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/18207
Acceso en línea:https://www.frontiersin.org/articles/10.3389/fvets.2022.1035036/full
https://hdl.handle.net/10612/18207
Access Level:acceso abierto
Palabra clave:Veterinaria
Doppler
Fertility
Ovine
Semen collection frequency
Sperm quality
Ultrasonography
3109 Ciencias Veterinarias
3104.11 Reproducción
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spelling Application of ultrasound technique to evaluate the testicular function and its correlation to the sperm quality after different collection frequency in ramsMontes Garrido, RafaelFernández Riesco, MartaAnel López, LuisNeila Montero, MartaPalacín Martínez, CristinaBoixo, Juan CarlosPaz Cabello, Paulino deOrtega Ferrusola, CristinaHassan, Mohamed A. A.Anel Rodríguez, LuisÁlvarez García, MercedesVeterinariaDopplerFertilityOvineSemen collection frequencySperm qualityUltrasonography3109 Ciencias Veterinarias3104.11 Reproducción[EN] The frequency of semen collection is a crucial factor to consider in the rams performance inside breeding centers workout. To evaluate this factor, ram Breeding Soundness Evaluation could include sperm quality evaluation and new predictive and non-invasive tools such as ultrasound technique. In this work, an advanced ultrasonography technology, analyzing the testicular volume, echotexture, and vascular function, was used in three different frequencies of semen collection (abstinence frequency, AF; standard frequency, SF; and intensive frequency, IF). Semen samples were cooled (15◦C, 6 h) and evaluated in terms of production, motility, viability, apoptosis, and content of reactive oxygen species. Correlation coe cients were calculated between ultrasonography measurements of echotexture and blood flow and sperm quality parameters. Our results showed an increase in the testicular echotexture when the frequency of semen collection was intensified. Doppler parameters (PSV, RI, PI, TABF) increased (P ≤ 0.05) when the frequency of semen collection was intensified. The sperm motility and functionality decreased in the samples of IF (P ≤ 0.05), evidencing the frequency of semen collection’s influence. Moreover, moderate positive correlations were established among echotexture and different Doppler parameters with motility parameters in SF. Furthermore, the influence of abstinence days on AI success was analyzed in a field assay. The highest fertility rates were obtained when males had two to five abstinence days. To conclude, frequency of semen collection could be influenced in terms of semen quantity and sperm quality, showing changes in parenchyma echotexture and testicular vascularization. The standard semen collection frequency was the most adequate option. In addition, ultrasonography may be a predictive tool for estimating variations in the sperm quality of donor rams subjected to different frequencies of semen collection in reproduction centers.SIFrontiersMedicina y Cirugia AnimalFacultad de Veterinaria2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://www.frontiersin.org/articles/10.3389/fvets.2022.1035036/fullhttps://hdl.handle.net/10612/18207reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad Rey Juan CarlosEspañolinfo:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/182072026-06-24T12:43:27Z
dc.title.none.fl_str_mv Application of ultrasound technique to evaluate the testicular function and its correlation to the sperm quality after different collection frequency in rams
title Application of ultrasound technique to evaluate the testicular function and its correlation to the sperm quality after different collection frequency in rams
spellingShingle Application of ultrasound technique to evaluate the testicular function and its correlation to the sperm quality after different collection frequency in rams
Montes Garrido, Rafael
Veterinaria
Doppler
Fertility
Ovine
Semen collection frequency
Sperm quality
Ultrasonography
3109 Ciencias Veterinarias
3104.11 Reproducción
title_short Application of ultrasound technique to evaluate the testicular function and its correlation to the sperm quality after different collection frequency in rams
title_full Application of ultrasound technique to evaluate the testicular function and its correlation to the sperm quality after different collection frequency in rams
title_fullStr Application of ultrasound technique to evaluate the testicular function and its correlation to the sperm quality after different collection frequency in rams
title_full_unstemmed Application of ultrasound technique to evaluate the testicular function and its correlation to the sperm quality after different collection frequency in rams
title_sort Application of ultrasound technique to evaluate the testicular function and its correlation to the sperm quality after different collection frequency in rams
dc.creator.none.fl_str_mv Montes Garrido, Rafael
Fernández Riesco, Marta
Anel López, Luis
Neila Montero, Marta
Palacín Martínez, Cristina
Boixo, Juan Carlos
Paz Cabello, Paulino de
Ortega Ferrusola, Cristina
Hassan, Mohamed A. A.
Anel Rodríguez, Luis
Álvarez García, Mercedes
author Montes Garrido, Rafael
author_facet Montes Garrido, Rafael
Fernández Riesco, Marta
Anel López, Luis
Neila Montero, Marta
Palacín Martínez, Cristina
Boixo, Juan Carlos
Paz Cabello, Paulino de
Ortega Ferrusola, Cristina
Hassan, Mohamed A. A.
Anel Rodríguez, Luis
Álvarez García, Mercedes
author_role author
author2 Fernández Riesco, Marta
Anel López, Luis
Neila Montero, Marta
Palacín Martínez, Cristina
Boixo, Juan Carlos
Paz Cabello, Paulino de
Ortega Ferrusola, Cristina
Hassan, Mohamed A. A.
Anel Rodríguez, Luis
Álvarez García, Mercedes
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Medicina y Cirugia Animal
Facultad de Veterinaria
dc.subject.none.fl_str_mv Veterinaria
Doppler
Fertility
Ovine
Semen collection frequency
Sperm quality
Ultrasonography
3109 Ciencias Veterinarias
3104.11 Reproducción
topic Veterinaria
Doppler
Fertility
Ovine
Semen collection frequency
Sperm quality
Ultrasonography
3109 Ciencias Veterinarias
3104.11 Reproducción
description [EN] The frequency of semen collection is a crucial factor to consider in the rams performance inside breeding centers workout. To evaluate this factor, ram Breeding Soundness Evaluation could include sperm quality evaluation and new predictive and non-invasive tools such as ultrasound technique. In this work, an advanced ultrasonography technology, analyzing the testicular volume, echotexture, and vascular function, was used in three different frequencies of semen collection (abstinence frequency, AF; standard frequency, SF; and intensive frequency, IF). Semen samples were cooled (15◦C, 6 h) and evaluated in terms of production, motility, viability, apoptosis, and content of reactive oxygen species. Correlation coe cients were calculated between ultrasonography measurements of echotexture and blood flow and sperm quality parameters. Our results showed an increase in the testicular echotexture when the frequency of semen collection was intensified. Doppler parameters (PSV, RI, PI, TABF) increased (P ≤ 0.05) when the frequency of semen collection was intensified. The sperm motility and functionality decreased in the samples of IF (P ≤ 0.05), evidencing the frequency of semen collection’s influence. Moreover, moderate positive correlations were established among echotexture and different Doppler parameters with motility parameters in SF. Furthermore, the influence of abstinence days on AI success was analyzed in a field assay. The highest fertility rates were obtained when males had two to five abstinence days. To conclude, frequency of semen collection could be influenced in terms of semen quantity and sperm quality, showing changes in parenchyma echotexture and testicular vascularization. The standard semen collection frequency was the most adequate option. In addition, ultrasonography may be a predictive tool for estimating variations in the sperm quality of donor rams subjected to different frequencies of semen collection in reproduction centers.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.frontiersin.org/articles/10.3389/fvets.2022.1035036/full
https://hdl.handle.net/10612/18207
url https://www.frontiersin.org/articles/10.3389/fvets.2022.1035036/full
https://hdl.handle.net/10612/18207
dc.language.none.fl_str_mv Español
language_invalid_str_mv Español
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad Rey Juan Carlos
instname_str Universidad Rey Juan Carlos
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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