Osteoblasts Interaction with PLGA Membranes Functionalized with Titanium Film Nanolayer by PECVD. In vitro Assessment of Surface Influence on Cell Adhesion during Initial Cell to Material Interaction

New biomaterials for Guided Bone Regeneration (GBR), both resorbable and non-resorbable, are being developed to stimulate bone tissue formation. Thus, the in vitro study of cell behavior towards material surface properties turns a prerequisite to assess both biocompatibility and bioactivity of any m...

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Autores: Terriza Fernández, Antonia, Vilches Pérez, José Ignacio, González Caballero, Juan Luis, Orden, Emilio de la, Yubero Valencia, Francisco, Barranco Quero, Ángel, Rodríguez González-Elipe, Agustín, Vilches, José, Salido, Mercedes
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/63954
Acceso en línea:http://hdl.handle.net/11441/63954
https://doi.org/10.3390/ma7031687
Access Level:acceso abierto
Palabra clave:Adhesion
Cell culture
Osteoblasts
Cell-material interaction
Plasma enhanced chemical vapor deposition (PECVD)
Titanium
Nanolayers
Functionalization
PLGA
Biomedical
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spelling Osteoblasts Interaction with PLGA Membranes Functionalized with Titanium Film Nanolayer by PECVD. In vitro Assessment of Surface Influence on Cell Adhesion during Initial Cell to Material InteractionTerriza Fernández, AntoniaVilches Pérez, José IgnacioGonzález Caballero, Juan LuisOrden, Emilio de laYubero Valencia, FranciscoBarranco Quero, ÁngelRodríguez González-Elipe, AgustínVilches, JoséSalido, MercedesAdhesionCell cultureOsteoblastsCell-material interactionPlasma enhanced chemical vapor deposition (PECVD)TitaniumNanolayersFunctionalizationPLGABiomedicalNew biomaterials for Guided Bone Regeneration (GBR), both resorbable and non-resorbable, are being developed to stimulate bone tissue formation. Thus, the in vitro study of cell behavior towards material surface properties turns a prerequisite to assess both biocompatibility and bioactivity of any material intended to be used for clinical purposes. For this purpose, we have developed in vitro studies on normal human osteoblasts (HOB®) HOB® osteoblasts grown on a resorbable Poly (lactide-co-glycolide) (PLGA) membrane foil functionalized by a very thin film (around 15 nm) of TiO2 (i.e., TiO2/PLGA membranes), designed to be used as barrier membrane. To avoid any alteration of the membranes, the titanium films were deposited at room temperature in one step by plasma enhanced chemical vapour deposition. Characterization of the functionalized membranes proved that the thin titanium layer completely covers the PLGA foils that remains practically unmodified in their interior after the deposition process and stands the standard sterilization protocols. Both morphological changes and cytoskeletal reorganization, together with the focal adhesion development observed in HOB osteoblasts, significantly related to TiO2 treated PLGA in which the Ti deposition method described has revealed to be a valuable tool to increase bioactivity of PLGA membranes, by combining cell nanotopography cues with the incorporation of bioactive factors.Junta de Andalucía P09CTS5189Ministerio de Ciencia e Innovación FIS PI 0900508Ministerio de Ciencia e Innovación CONSOLIDER CSD2008-00023Ministerio de Ciencia e Innovación MAT2010-21228MDPI2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/11441/63954https://doi.org/10.3390/ma7031687reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésMaterials, 7 (3), 1687-1708.P09CTS5189FIS PI 0900508CSD2008-00023MAT2010-21228http://dx.doi.org/10.3390/ma7031687info:eu-repo/semantics/openAccessoai:idus.us.es:11441/639542026-06-17T12:51:07Z
dc.title.none.fl_str_mv Osteoblasts Interaction with PLGA Membranes Functionalized with Titanium Film Nanolayer by PECVD. In vitro Assessment of Surface Influence on Cell Adhesion during Initial Cell to Material Interaction
title Osteoblasts Interaction with PLGA Membranes Functionalized with Titanium Film Nanolayer by PECVD. In vitro Assessment of Surface Influence on Cell Adhesion during Initial Cell to Material Interaction
spellingShingle Osteoblasts Interaction with PLGA Membranes Functionalized with Titanium Film Nanolayer by PECVD. In vitro Assessment of Surface Influence on Cell Adhesion during Initial Cell to Material Interaction
Terriza Fernández, Antonia
Adhesion
Cell culture
Osteoblasts
Cell-material interaction
Plasma enhanced chemical vapor deposition (PECVD)
Titanium
Nanolayers
Functionalization
PLGA
Biomedical
title_short Osteoblasts Interaction with PLGA Membranes Functionalized with Titanium Film Nanolayer by PECVD. In vitro Assessment of Surface Influence on Cell Adhesion during Initial Cell to Material Interaction
title_full Osteoblasts Interaction with PLGA Membranes Functionalized with Titanium Film Nanolayer by PECVD. In vitro Assessment of Surface Influence on Cell Adhesion during Initial Cell to Material Interaction
title_fullStr Osteoblasts Interaction with PLGA Membranes Functionalized with Titanium Film Nanolayer by PECVD. In vitro Assessment of Surface Influence on Cell Adhesion during Initial Cell to Material Interaction
title_full_unstemmed Osteoblasts Interaction with PLGA Membranes Functionalized with Titanium Film Nanolayer by PECVD. In vitro Assessment of Surface Influence on Cell Adhesion during Initial Cell to Material Interaction
title_sort Osteoblasts Interaction with PLGA Membranes Functionalized with Titanium Film Nanolayer by PECVD. In vitro Assessment of Surface Influence on Cell Adhesion during Initial Cell to Material Interaction
dc.creator.none.fl_str_mv Terriza Fernández, Antonia
Vilches Pérez, José Ignacio
González Caballero, Juan Luis
Orden, Emilio de la
Yubero Valencia, Francisco
Barranco Quero, Ángel
Rodríguez González-Elipe, Agustín
Vilches, José
Salido, Mercedes
author Terriza Fernández, Antonia
author_facet Terriza Fernández, Antonia
Vilches Pérez, José Ignacio
González Caballero, Juan Luis
Orden, Emilio de la
Yubero Valencia, Francisco
Barranco Quero, Ángel
Rodríguez González-Elipe, Agustín
Vilches, José
Salido, Mercedes
author_role author
author2 Vilches Pérez, José Ignacio
González Caballero, Juan Luis
Orden, Emilio de la
Yubero Valencia, Francisco
Barranco Quero, Ángel
Rodríguez González-Elipe, Agustín
Vilches, José
Salido, Mercedes
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Adhesion
Cell culture
Osteoblasts
Cell-material interaction
Plasma enhanced chemical vapor deposition (PECVD)
Titanium
Nanolayers
Functionalization
PLGA
Biomedical
topic Adhesion
Cell culture
Osteoblasts
Cell-material interaction
Plasma enhanced chemical vapor deposition (PECVD)
Titanium
Nanolayers
Functionalization
PLGA
Biomedical
description New biomaterials for Guided Bone Regeneration (GBR), both resorbable and non-resorbable, are being developed to stimulate bone tissue formation. Thus, the in vitro study of cell behavior towards material surface properties turns a prerequisite to assess both biocompatibility and bioactivity of any material intended to be used for clinical purposes. For this purpose, we have developed in vitro studies on normal human osteoblasts (HOB®) HOB® osteoblasts grown on a resorbable Poly (lactide-co-glycolide) (PLGA) membrane foil functionalized by a very thin film (around 15 nm) of TiO2 (i.e., TiO2/PLGA membranes), designed to be used as barrier membrane. To avoid any alteration of the membranes, the titanium films were deposited at room temperature in one step by plasma enhanced chemical vapour deposition. Characterization of the functionalized membranes proved that the thin titanium layer completely covers the PLGA foils that remains practically unmodified in their interior after the deposition process and stands the standard sterilization protocols. Both morphological changes and cytoskeletal reorganization, together with the focal adhesion development observed in HOB osteoblasts, significantly related to TiO2 treated PLGA in which the Ti deposition method described has revealed to be a valuable tool to increase bioactivity of PLGA membranes, by combining cell nanotopography cues with the incorporation of bioactive factors.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11441/63954
https://doi.org/10.3390/ma7031687
url http://hdl.handle.net/11441/63954
https://doi.org/10.3390/ma7031687
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Materials, 7 (3), 1687-1708.
P09CTS5189
FIS PI 0900508
CSD2008-00023
MAT2010-21228
http://dx.doi.org/10.3390/ma7031687
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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