Comparative ex vivo responses of ovine and bovine polymorphonuclear neutrophils induced by Neospora caninum tachyzoites

[EN] Neospora caninum infection causes reproductive failure in ruminants with a traditionally higher incidence of bovine neosporosis compared to the ovine system. Differences in innate immune response could explain this observation. We here focused our study on the comparison of the N. caninum-induc...

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Autores: Gutiérrez Expósito, Daniel, Silva, Liliana M. R., Wagner, Henrik, Gärtner, U., Hermosilla, Carlos, Taubert, Anja, Conejeros, I.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Institución:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:dnet:buleria_____::a11d832c35f5dc81795fa907b0f96358
Acceso en línea:https://hdl.handle.net/10612/28425
Access Level:acceso abierto
Palabra clave:Sanidad animal
NETs formation
ROS
Innate immune response
Neospora caninum
Cattle
Sheep
3109 Ciencias Veterinarias
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spelling Comparative ex vivo responses of ovine and bovine polymorphonuclear neutrophils induced by Neospora caninum tachyzoitesGutiérrez Expósito, DanielSilva, Liliana M. R.Wagner, HenrikGärtner, U.Hermosilla, CarlosTaubert, AnjaConejeros, I.Sanidad animalNETs formationROSInnate immune responseNeospora caninumCattleSheep3109 Ciencias Veterinarias[EN] Neospora caninum infection causes reproductive failure in ruminants with a traditionally higher incidence of bovine neosporosis compared to the ovine system. Differences in innate immune response could explain this observation. We here focused our study on the comparison of the N. caninum-induced response on bovine and ovine polymorphonuclear neutrophils (PMN) that might play a key role in the pathogenesis of neosporosis in both small and large ruminants. We here examined and compared in parallel PMN responses of healthy adult sheep (n = 6) and cows (n = 6) after exposure to N. caninum tachyzoites (ratio 1:4). PMN activation was evaluated by induction of NETosis, determined by immunofluorescence and scanning electron microscopy (SEM), extracellular reactive oxygen species (ROS) production via Amplex Red assays, and oxygen consumption rates (OCR) and proton efflux rates (PER) quantified by Seahorse XF technology. N. caninum tachyzoite-driven percentage of NETotic PMN was higher in the bovine system (24.4%) when compared to sheep (11.3%). For both, SEM analyses confirmed PMN activation and the formation of NET structures upon N. caninum tachyzoite exposure. The increase in tachyzoite-mediated ROS production proved higher in cattle than in sheep and these data were in line with significantly higher PER on bovine PMN indicating a differential glycolytic activity upon N. caninum exposure. Overall, this study documents early (minutes) and mid-late (hours) ovine and bovine PMN reactions after being exposed to N. caninum tachyzoites. The fundamental information here given contributes to the understanding of neosporosis in cows and sheep that should be complemented with in vivo studies.SIDGE has been financially supported by the Ministry of Universities of Spain (MCIN) (Grant “Ayuda para la Recualificación del Profesorado”) and, as appropriate, by “European Union NextGenerationEU/PRTR”.ElsevierSanidad AnimalFacultad de Veterinaria2026info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://hdl.handle.net/10612/28425reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónIngléshttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:dnet:buleria_____::a11d832c35f5dc81795fa907b0f963582026-06-24T12:43:27Z
dc.title.none.fl_str_mv Comparative ex vivo responses of ovine and bovine polymorphonuclear neutrophils induced by Neospora caninum tachyzoites
title Comparative ex vivo responses of ovine and bovine polymorphonuclear neutrophils induced by Neospora caninum tachyzoites
spellingShingle Comparative ex vivo responses of ovine and bovine polymorphonuclear neutrophils induced by Neospora caninum tachyzoites
Gutiérrez Expósito, Daniel
Sanidad animal
NETs formation
ROS
Innate immune response
Neospora caninum
Cattle
Sheep
3109 Ciencias Veterinarias
title_short Comparative ex vivo responses of ovine and bovine polymorphonuclear neutrophils induced by Neospora caninum tachyzoites
title_full Comparative ex vivo responses of ovine and bovine polymorphonuclear neutrophils induced by Neospora caninum tachyzoites
title_fullStr Comparative ex vivo responses of ovine and bovine polymorphonuclear neutrophils induced by Neospora caninum tachyzoites
title_full_unstemmed Comparative ex vivo responses of ovine and bovine polymorphonuclear neutrophils induced by Neospora caninum tachyzoites
title_sort Comparative ex vivo responses of ovine and bovine polymorphonuclear neutrophils induced by Neospora caninum tachyzoites
dc.creator.none.fl_str_mv Gutiérrez Expósito, Daniel
Silva, Liliana M. R.
Wagner, Henrik
Gärtner, U.
Hermosilla, Carlos
Taubert, Anja
Conejeros, I.
author Gutiérrez Expósito, Daniel
author_facet Gutiérrez Expósito, Daniel
Silva, Liliana M. R.
Wagner, Henrik
Gärtner, U.
Hermosilla, Carlos
Taubert, Anja
Conejeros, I.
author_role author
author2 Silva, Liliana M. R.
Wagner, Henrik
Gärtner, U.
Hermosilla, Carlos
Taubert, Anja
Conejeros, I.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sanidad Animal
Facultad de Veterinaria
dc.subject.none.fl_str_mv Sanidad animal
NETs formation
ROS
Innate immune response
Neospora caninum
Cattle
Sheep
3109 Ciencias Veterinarias
topic Sanidad animal
NETs formation
ROS
Innate immune response
Neospora caninum
Cattle
Sheep
3109 Ciencias Veterinarias
description [EN] Neospora caninum infection causes reproductive failure in ruminants with a traditionally higher incidence of bovine neosporosis compared to the ovine system. Differences in innate immune response could explain this observation. We here focused our study on the comparison of the N. caninum-induced response on bovine and ovine polymorphonuclear neutrophils (PMN) that might play a key role in the pathogenesis of neosporosis in both small and large ruminants. We here examined and compared in parallel PMN responses of healthy adult sheep (n = 6) and cows (n = 6) after exposure to N. caninum tachyzoites (ratio 1:4). PMN activation was evaluated by induction of NETosis, determined by immunofluorescence and scanning electron microscopy (SEM), extracellular reactive oxygen species (ROS) production via Amplex Red assays, and oxygen consumption rates (OCR) and proton efflux rates (PER) quantified by Seahorse XF technology. N. caninum tachyzoite-driven percentage of NETotic PMN was higher in the bovine system (24.4%) when compared to sheep (11.3%). For both, SEM analyses confirmed PMN activation and the formation of NET structures upon N. caninum tachyzoite exposure. The increase in tachyzoite-mediated ROS production proved higher in cattle than in sheep and these data were in line with significantly higher PER on bovine PMN indicating a differential glycolytic activity upon N. caninum exposure. Overall, this study documents early (minutes) and mid-late (hours) ovine and bovine PMN reactions after being exposed to N. caninum tachyzoites. The fundamental information here given contributes to the understanding of neosporosis in cows and sheep that should be complemented with in vivo studies.
publishDate 2026
dc.date.none.fl_str_mv 2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/10612/28425
url https://hdl.handle.net/10612/28425
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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