Determination of crAssphage in water samples and applicability for tracking human fecal pollution

In recent decades, considerable effort has been devoted to finding microbial source-tracking (MST) markers that are suitable to assess the health risks of faecally polluted waters, with no universal marker reported so far. In this study, the abundance and prevalence of a crAssphage-derived DNA marke...

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Detalles Bibliográficos
Autores: García Aljaro, Cristina, Ballesté Pau, Elisenda, Muniesa Pérez, Ma. Teresa, Jofre i Torroella, Joan
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/191900
Acceso en línea:https://hdl.handle.net/2445/191900
Access Level:acceso abierto
Palabra clave:Contaminació de l'aigua
Excrements
Bacteris
Water pollution
Feces
Bacteria
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spelling Determination of crAssphage in water samples and applicability for tracking human fecal pollutionGarcía Aljaro, CristinaBallesté Pau, ElisendaMuniesa Pérez, Ma. TeresaJofre i Torroella, JoanContaminació de l'aiguaExcrementsBacterisWater pollutionFecesBacteriaIn recent decades, considerable effort has been devoted to finding microbial source-tracking (MST) markers that are suitable to assess the health risks of faecally polluted waters, with no universal marker reported so far. In this study, the abundance and prevalence of a crAssphage-derived DNA marker in wastewaters of human and animal origins were studied by a new qPCR assay with the ultimate aim of assessing its potential as an MST marker. crAssphage showed up to 106 GC/ml in the sewage samples of human origin, in both the total DNA and the viral DNA fraction. In wastewaters containing animal faecal remains, 39% of the samples were negative for the presence of the crAssphage sequence, while those showing positive results (41% of the samples) were at least 1 log10 unit lower than the samples of human origin. Noteworthy, the log10 values of the ratio (R) crAssphage (GC/ml)/Escherichia coli (CFU/ml) varied significantly depending on the human or animal origin (R > 1.5 for human samples and R < −1.5 for animal wastewater samples. This study opens the way for further research to explore if different specific animal variants of crAssphage exist and whether other zones of the crAssphage genome are better suited to source discrimination.Applied Microbiology International2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/191900Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1111/1751-7915.12841Microbial Biotechnology, 2017, vol. 10, num. 6, p. 1775-1780https://doi.org/10.1111/1751-7915.12841cc-by (c) García Aljaro, Cristina et al., 2017https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1919002026-05-27T06:46:51Z
dc.title.none.fl_str_mv Determination of crAssphage in water samples and applicability for tracking human fecal pollution
title Determination of crAssphage in water samples and applicability for tracking human fecal pollution
spellingShingle Determination of crAssphage in water samples and applicability for tracking human fecal pollution
García Aljaro, Cristina
Contaminació de l'aigua
Excrements
Bacteris
Water pollution
Feces
Bacteria
title_short Determination of crAssphage in water samples and applicability for tracking human fecal pollution
title_full Determination of crAssphage in water samples and applicability for tracking human fecal pollution
title_fullStr Determination of crAssphage in water samples and applicability for tracking human fecal pollution
title_full_unstemmed Determination of crAssphage in water samples and applicability for tracking human fecal pollution
title_sort Determination of crAssphage in water samples and applicability for tracking human fecal pollution
dc.creator.none.fl_str_mv García Aljaro, Cristina
Ballesté Pau, Elisenda
Muniesa Pérez, Ma. Teresa
Jofre i Torroella, Joan
author García Aljaro, Cristina
author_facet García Aljaro, Cristina
Ballesté Pau, Elisenda
Muniesa Pérez, Ma. Teresa
Jofre i Torroella, Joan
author_role author
author2 Ballesté Pau, Elisenda
Muniesa Pérez, Ma. Teresa
Jofre i Torroella, Joan
author2_role author
author
author
dc.subject.none.fl_str_mv Contaminació de l'aigua
Excrements
Bacteris
Water pollution
Feces
Bacteria
topic Contaminació de l'aigua
Excrements
Bacteris
Water pollution
Feces
Bacteria
description In recent decades, considerable effort has been devoted to finding microbial source-tracking (MST) markers that are suitable to assess the health risks of faecally polluted waters, with no universal marker reported so far. In this study, the abundance and prevalence of a crAssphage-derived DNA marker in wastewaters of human and animal origins were studied by a new qPCR assay with the ultimate aim of assessing its potential as an MST marker. crAssphage showed up to 106 GC/ml in the sewage samples of human origin, in both the total DNA and the viral DNA fraction. In wastewaters containing animal faecal remains, 39% of the samples were negative for the presence of the crAssphage sequence, while those showing positive results (41% of the samples) were at least 1 log10 unit lower than the samples of human origin. Noteworthy, the log10 values of the ratio (R) crAssphage (GC/ml)/Escherichia coli (CFU/ml) varied significantly depending on the human or animal origin (R > 1.5 for human samples and R < −1.5 for animal wastewater samples. This study opens the way for further research to explore if different specific animal variants of crAssphage exist and whether other zones of the crAssphage genome are better suited to source discrimination.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/191900
url https://hdl.handle.net/2445/191900
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1111/1751-7915.12841
Microbial Biotechnology, 2017, vol. 10, num. 6, p. 1775-1780
https://doi.org/10.1111/1751-7915.12841
dc.rights.none.fl_str_mv cc-by (c) García Aljaro, Cristina et al., 2017
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) García Aljaro, Cristina et al., 2017
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Applied Microbiology International
publisher.none.fl_str_mv Applied Microbiology International
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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