Cytotoxicity evaluation of anatase and rutile TiO2 thin films on CHO-K1 cells in vitro

This article belongs to the Section Biomaterials.-- et al.

Detalles Bibliográficos
Autores: Cervantes, Blanca, Soto, Enrique
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/151106
Acceso en línea:http://hdl.handle.net/10261/151106
Access Level:acceso abierto
Palabra clave:MTT
Sensors
Biocompatibility
Titanium dioxide
Titanium
Cytotoxicity
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spelling Cytotoxicity evaluation of anatase and rutile TiO2 thin films on CHO-K1 cells in vitroCervantes, BlancaSoto, EnriqueMTTSensorsBiocompatibilityTitanium dioxideTitaniumCytotoxicityThis article belongs to the Section Biomaterials.-- et al.Cytotoxicity of titanium dioxide (TiO2) thin films on Chinese hamster ovary (CHO-K1) cells was evaluated after 24, 48 and 72 h of culture. The TiO2 thin films were deposited using direct current magnetron sputtering. These films were post-deposition annealed at different temperatures (300, 500 and 800 °C) toward the anatase to rutile phase transformation. The root-mean-square (RMS) surface roughness of TiO2 films went from 2.8 to 8.08 nm when the annealing temperature was increased from 300 to 800 °C. Field emission scanning electron microscopy (FESEM) results showed that the TiO2 films’ thickness values fell within the nanometer range (290–310 nm). Based on the results of the tetrazolium dye and trypan blue assays, we found that TiO2 thin films showed no cytotoxicity after the aforementioned culture times at which cell viability was greater than 98%. Independently of the annealing temperature of the TiO2 thin films, the number of CHO-K1 cells on the control substrate and on all TiO2 thin films was greater after 48 or 72 h than it was after 24 h; the highest cell survival rate was observed in TiO2 films annealed at 800 °C. These results indicate that TiO2 thin films do not affect mitochondrial function and proliferation of CHO-K1 cells, and back up the use of TiO2 thin films in biomedical science.This work was financed by Secretaría de Educación Pública (SEP) “Programa de Mejoramiento del Posgrado” (PROMEP) posdoctoral grant DSA/103.5/14/5782 to Blanca Cervantes, grant PROMEP-RED “Estudio de dispositivos electrónicos y electromecánicos con aplicación en fisiología y optoelectrónica” and “Programa Institucional de Fortalecimiento al Posgrado” (PIFI) 2013-2014, by Centro Universitario de Vinculación y Transferencia de Tecnología de la Benemérita Universidad Autónoma de Puebla (BUAP) grants DITCo32 and DITCo 2016-9 to Enrique Soto, and by National Council of Science and Technology of México (CONACyT) grant CA Neurociencias 229866.Peer ReviewedMultidisciplinary Digital Publishing InstituteSecretaría de Educación Pública (México)Consejo Superior de Investigaciones Científicas (España)Benemérita Universidad Autónoma de PueblaConsejo Nacional de Ciencia y Tecnología (México)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2017201720162017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/151106reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3390/ma9080619Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1511062026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cytotoxicity evaluation of anatase and rutile TiO2 thin films on CHO-K1 cells in vitro
title Cytotoxicity evaluation of anatase and rutile TiO2 thin films on CHO-K1 cells in vitro
spellingShingle Cytotoxicity evaluation of anatase and rutile TiO2 thin films on CHO-K1 cells in vitro
Cervantes, Blanca
MTT
Sensors
Biocompatibility
Titanium dioxide
Titanium
Cytotoxicity
title_short Cytotoxicity evaluation of anatase and rutile TiO2 thin films on CHO-K1 cells in vitro
title_full Cytotoxicity evaluation of anatase and rutile TiO2 thin films on CHO-K1 cells in vitro
title_fullStr Cytotoxicity evaluation of anatase and rutile TiO2 thin films on CHO-K1 cells in vitro
title_full_unstemmed Cytotoxicity evaluation of anatase and rutile TiO2 thin films on CHO-K1 cells in vitro
title_sort Cytotoxicity evaluation of anatase and rutile TiO2 thin films on CHO-K1 cells in vitro
dc.creator.none.fl_str_mv Cervantes, Blanca
Soto, Enrique
author Cervantes, Blanca
author_facet Cervantes, Blanca
Soto, Enrique
author_role author
author2 Soto, Enrique
author2_role author
dc.contributor.none.fl_str_mv Secretaría de Educación Pública (México)
Consejo Superior de Investigaciones Científicas (España)
Benemérita Universidad Autónoma de Puebla
Consejo Nacional de Ciencia y Tecnología (México)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv MTT
Sensors
Biocompatibility
Titanium dioxide
Titanium
Cytotoxicity
topic MTT
Sensors
Biocompatibility
Titanium dioxide
Titanium
Cytotoxicity
description This article belongs to the Section Biomaterials.-- et al.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/151106
url http://hdl.handle.net/10261/151106
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3390/ma9080619
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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