Dietary Sugars Alter Hepatic Fatty Acid Oxidation via Transcriptional and Post-translational Modifications of Mitochondrial Proteins

Dietary sugars, fructose and glucose, promote hepatic de novo lipogenesis and modify the effects of a high-fat diet (HFD) on the development of insulin resistance. Here, we show that fructose and glucose supplementation of an HFD exert divergent effects on hepatic mitochondrial function and fatty ac...

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Bibliographic Details
Authors: Softic, Samir, Meyer, Jesse G., Wang, Guo-Xiao, Gupta, Manoj K, Batista, Thiago M, Lauritzen, Hans P M M, Fujisaka, Shiho, Serra i Cucurull, Dolors, Herrero Rodríguez, Laura, Willoughby, Jennifer, Fitzgerald, Kevin, Ilkayeva, Olga, Newgard, Christopher B, Gibson, Bradford W, Schilling, Birgit, Cohen, David E, Kahn, C. Ronald
Format: article
Status:Versión aceptada para publicación
Publication Date:2019
Country:España
Institution:Universidad de Barcelona
Repository:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/167638
Online Access:https://hdl.handle.net/2445/167638
Access Level:Open access
Keyword:Malalties del fetge
Obesitat
Fructosa
Glucosa
Liver diseases
Obesity
Fructose
Glucose
Description
Summary:Dietary sugars, fructose and glucose, promote hepatic de novo lipogenesis and modify the effects of a high-fat diet (HFD) on the development of insulin resistance. Here, we show that fructose and glucose supplementation of an HFD exert divergent effects on hepatic mitochondrial function and fatty acid oxidation. This is mediated via three different nodes of regulation, including differential effects on malonyl-CoA levels, effects on mitochondrial size/protein abundance, and acetylation of mitochondrial proteins. HFD- and HFD plus fructose-fed mice have decreased CTP1a activity, the rate-limiting enzyme of fatty acid oxidation, whereas knockdown of fructose metabolism increases CPT1a and its acylcarnitine products. Furthermore, fructose-supplemented HFD leads to increased acetylation of ACADL and CPT1a, which is associated with decreased fat metabolism. In summary, dietary fructose, but not glucose, supplementation of HFD impairs mitochondrial size, function, and protein acetylation, resulting in decreased fatty acid oxidation and development of metabolic dysregulation.

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