Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in a Spanish multicentric case-control study

BACKGROUND: Epstein-Barr virus (EBV)-related malignancies harbour distinct serological responses to EBV antigens. We hypothesized that EBV serological patterns can be useful to identify different stages of chronic lymphocytic leukemia. METHODS: Information on 150 cases with chronic lymphocytic leuke...

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Autores: Casabonne, Delphine, Benavente, Yolanda, Robles, Claudia, Costas, Laura, Alonso Sanz, Esther, González Barca, Eva, Tardón García, Adonina, Dierssen Sotos, Trinidad|||0000-0002-6127-0077, Gimeno Vázquez, Eva, Aymerich Gregorio, Marta, Campo Güerri, Elias, Castaño Vinyals, Gemma, Aragonés Sanz, Nuria, Pollán, Marina, Kogevinas, Manolis, Juwana, Hedy, Middeldorp, Jaap, Sanjosé Llongueras, Silvia de
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Universidad de Cantabria (UC)
Repositorio:UCrea Repositorio Abierto de la Universidad de Cantabria
Idioma:inglés
OAI Identifier:oai:repositorio.unican.es:10902/6402
Acceso en línea:http://hdl.handle.net/10902/6402
Access Level:acceso abierto
Palabra clave:Case-control
Chronic lymphocytic leukemia
Epstein-Barr virus
Serology
Smoking
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spelling Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in a Spanish multicentric case-control studyCasabonne, DelphineBenavente, YolandaRobles, ClaudiaCostas, LauraAlonso Sanz, EstherGonzález Barca, EvaTardón García, AdoninaDierssen Sotos, Trinidad|||0000-0002-6127-0077Gimeno Vázquez, EvaAymerich Gregorio, MartaCampo Güerri, EliasCastaño Vinyals, GemmaAragonés Sanz, NuriaPollán, MarinaKogevinas, ManolisJuwana, HedyMiddeldorp, JaapSanjosé Llongueras, Silvia deCase-controlChronic lymphocytic leukemiaEpstein-Barr virusSerologySmokingBACKGROUND: Epstein-Barr virus (EBV)-related malignancies harbour distinct serological responses to EBV antigens. We hypothesized that EBV serological patterns can be useful to identify different stages of chronic lymphocytic leukemia. METHODS: Information on 150 cases with chronic lymphocytic leukemia and 157 frequency-matched (by age, sex and region) population-based controls from a Spanish multicentre case-control study was obtained. EBV immunoglobulin G serostatus was evaluated through a peptide-based ELISA and further by immunoblot analysis to EBV early antigens (EA), nuclear antigen (EBNA1), VCA-p18, VCA-p40 and Zebra. Two independent individuals categorized the serological patterns of the western blot analysis. Patients with very high response and diversity in EBV-specific polypeptides, in particular with clear responses to EA-associated proteins, were categorized as having an abnormal reactive pattern (ab_EBV). Adjusted odds ratios (OR) and 95% confidence interval (CI) were estimated using logistic regression models. RESULTS: Almost all subjects were EBV-IgG positive (>95% of cases and controls) whereas ab_EBV patterns were detected in 23% of cases (N = 34) and 11% of controls (N = 17; OR: 2.44, 95% CI, 1.29 to 4.62; P = 0.006), particularly in intermediate/high risk patients. Although based on small numbers, the association was modified by smoking with a gradual reduction of ab_EBV-related OR for all Rai stages from never smokers to current smokers. CONCLUSIONS: Highly distinct EBV antibody diversity patterns revealed by immunoblot analysis were detected in cases compared to controls, detectable at very early stages of the disease and particularly among non smokers. This study provides further evidence of an abnormal immunological response against EBV in patients with chronic lymphocytic leukemia.BioMed CentralUniversidad de Cantabria20152015-02-09journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articlehttp://hdl.handle.net/10902/6402Infectious Agents and Cancer. 2015 Feb 9;10:5reponame:UCrea Repositorio Abierto de la Universidad de Cantabriainstname:Universidad de Cantabria (UC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttp://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:repositorio.unican.es:10902/64022026-06-02T12:39:31Z
dc.title.none.fl_str_mv Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in a Spanish multicentric case-control study
title Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in a Spanish multicentric case-control study
spellingShingle Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in a Spanish multicentric case-control study
Casabonne, Delphine
Case-control
Chronic lymphocytic leukemia
Epstein-Barr virus
Serology
Smoking
title_short Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in a Spanish multicentric case-control study
title_full Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in a Spanish multicentric case-control study
title_fullStr Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in a Spanish multicentric case-control study
title_full_unstemmed Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in a Spanish multicentric case-control study
title_sort Aberrant Epstein-Barr virus antibody patterns and chronic lymphocytic leukemia in a Spanish multicentric case-control study
dc.creator.none.fl_str_mv Casabonne, Delphine
Benavente, Yolanda
Robles, Claudia
Costas, Laura
Alonso Sanz, Esther
González Barca, Eva
Tardón García, Adonina
Dierssen Sotos, Trinidad|||0000-0002-6127-0077
Gimeno Vázquez, Eva
Aymerich Gregorio, Marta
Campo Güerri, Elias
Castaño Vinyals, Gemma
Aragonés Sanz, Nuria
Pollán, Marina
Kogevinas, Manolis
Juwana, Hedy
Middeldorp, Jaap
Sanjosé Llongueras, Silvia de
author Casabonne, Delphine
author_facet Casabonne, Delphine
Benavente, Yolanda
Robles, Claudia
Costas, Laura
Alonso Sanz, Esther
González Barca, Eva
Tardón García, Adonina
Dierssen Sotos, Trinidad|||0000-0002-6127-0077
Gimeno Vázquez, Eva
Aymerich Gregorio, Marta
Campo Güerri, Elias
Castaño Vinyals, Gemma
Aragonés Sanz, Nuria
Pollán, Marina
Kogevinas, Manolis
Juwana, Hedy
Middeldorp, Jaap
Sanjosé Llongueras, Silvia de
author_role author
author2 Benavente, Yolanda
Robles, Claudia
Costas, Laura
Alonso Sanz, Esther
González Barca, Eva
Tardón García, Adonina
Dierssen Sotos, Trinidad|||0000-0002-6127-0077
Gimeno Vázquez, Eva
Aymerich Gregorio, Marta
Campo Güerri, Elias
Castaño Vinyals, Gemma
Aragonés Sanz, Nuria
Pollán, Marina
Kogevinas, Manolis
Juwana, Hedy
Middeldorp, Jaap
Sanjosé Llongueras, Silvia de
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad de Cantabria
dc.subject.none.fl_str_mv Case-control
Chronic lymphocytic leukemia
Epstein-Barr virus
Serology
Smoking
topic Case-control
Chronic lymphocytic leukemia
Epstein-Barr virus
Serology
Smoking
description BACKGROUND: Epstein-Barr virus (EBV)-related malignancies harbour distinct serological responses to EBV antigens. We hypothesized that EBV serological patterns can be useful to identify different stages of chronic lymphocytic leukemia. METHODS: Information on 150 cases with chronic lymphocytic leukemia and 157 frequency-matched (by age, sex and region) population-based controls from a Spanish multicentre case-control study was obtained. EBV immunoglobulin G serostatus was evaluated through a peptide-based ELISA and further by immunoblot analysis to EBV early antigens (EA), nuclear antigen (EBNA1), VCA-p18, VCA-p40 and Zebra. Two independent individuals categorized the serological patterns of the western blot analysis. Patients with very high response and diversity in EBV-specific polypeptides, in particular with clear responses to EA-associated proteins, were categorized as having an abnormal reactive pattern (ab_EBV). Adjusted odds ratios (OR) and 95% confidence interval (CI) were estimated using logistic regression models. RESULTS: Almost all subjects were EBV-IgG positive (>95% of cases and controls) whereas ab_EBV patterns were detected in 23% of cases (N = 34) and 11% of controls (N = 17; OR: 2.44, 95% CI, 1.29 to 4.62; P = 0.006), particularly in intermediate/high risk patients. Although based on small numbers, the association was modified by smoking with a gradual reduction of ab_EBV-related OR for all Rai stages from never smokers to current smokers. CONCLUSIONS: Highly distinct EBV antibody diversity patterns revealed by immunoblot analysis were detected in cases compared to controls, detectable at very early stages of the disease and particularly among non smokers. This study provides further evidence of an abnormal immunological response against EBV in patients with chronic lymphocytic leukemia.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-02-09
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10902/6402
url http://hdl.handle.net/10902/6402
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
http://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Infectious Agents and Cancer. 2015 Feb 9;10:5
reponame:UCrea Repositorio Abierto de la Universidad de Cantabria
instname:Universidad de Cantabria (UC)
instname_str Universidad de Cantabria (UC)
reponame_str UCrea Repositorio Abierto de la Universidad de Cantabria
collection UCrea Repositorio Abierto de la Universidad de Cantabria
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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