Role of the small GTPase Rab27a during Herpes simplex virus infection of oligodendrocytic cells

Background: The morphogenesis of herpes simplex virus type 1 (HSV-1) comprises several events, of which some are not completely understood. It has been shown that HSV-1 glycoproteins accumulate in the trans-Golgi network (TGN) and in TGN-derived vesicles. It is also accepted that HSV-1 acquires its...

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Autores: Bello-Morales Arroyo, Ángeles Raquel, Crespillo, Antonio Jesús, Fraile-Ramos, Alberto, Tabares López, Enrique, Alcina, Antonio, López Guerrero, José Antonio
Tipo de recurso: artículo
Fecha de publicación:2012
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/662847
Acceso en línea:http://hdl.handle.net/10486/662847
https://dx.doi.org/10.1186/1471-2180-12-265
Access Level:acceso abierto
Palabra clave:HSV-1
Morphogenesis
Oligodendrocytes
Rab27a
Tegument
Viral egress
Biología y Biomedicina / Biología
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spelling Role of the small GTPase Rab27a during Herpes simplex virus infection of oligodendrocytic cellsBello-Morales Arroyo, Ángeles RaquelCrespillo, Antonio JesúsFraile-Ramos, AlbertoTabares López, EnriqueAlcina, AntonioLópez Guerrero, José AntonioHSV-1MorphogenesisOligodendrocytesRab27aTegumentViral egressBiología y Biomedicina / BiologíaBackground: The morphogenesis of herpes simplex virus type 1 (HSV-1) comprises several events, of which some are not completely understood. It has been shown that HSV-1 glycoproteins accumulate in the trans-Golgi network (TGN) and in TGN-derived vesicles. It is also accepted that HSV-1 acquires its final morphology through a secondary envelopment by budding into TGN-derived vesicles coated with viral glycoproteins and tegument proteins. Nevertheless, several aspects of this process remain elusive. The small GTPase Rab27a has been implicated in regulated exocytosis, and it seems to play a key role in certain membrane trafficking events. Rab27a also seems to be required for human cytomegalovirus assembly. However, despite the involvement of various Rab GTPases in HSV-1 envelopment, there is, to date, no data reported on the role of Rab27a in HSV-1 infection. Results: Herein, we show that Rab27a colocalized with GHSV-UL46, a tegument-tagged green fluorescent protein-HSV-1, in the TGN. In fact, this small GTPase colocalized with viral glycoproteins gH and gD in that compartment. Functional analysis through Rab27a depletion showed a significant decrease in the number of infected cells and viral production in Rab27a-silenced cells. Conclusions: Altogether, our results indicate that Rab27a plays an important role in HSV-1 infection of oligodendrocytic cellsThe AuthorsDepartamento de Biología MolecularFacultad de Ciencias20122012-11-21research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/662847https://dx.doi.org/10.1186/1471-2180-12-265reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6628472026-06-23T12:46:27Z
dc.title.none.fl_str_mv Role of the small GTPase Rab27a during Herpes simplex virus infection of oligodendrocytic cells
title Role of the small GTPase Rab27a during Herpes simplex virus infection of oligodendrocytic cells
spellingShingle Role of the small GTPase Rab27a during Herpes simplex virus infection of oligodendrocytic cells
Bello-Morales Arroyo, Ángeles Raquel
HSV-1
Morphogenesis
Oligodendrocytes
Rab27a
Tegument
Viral egress
Biología y Biomedicina / Biología
title_short Role of the small GTPase Rab27a during Herpes simplex virus infection of oligodendrocytic cells
title_full Role of the small GTPase Rab27a during Herpes simplex virus infection of oligodendrocytic cells
title_fullStr Role of the small GTPase Rab27a during Herpes simplex virus infection of oligodendrocytic cells
title_full_unstemmed Role of the small GTPase Rab27a during Herpes simplex virus infection of oligodendrocytic cells
title_sort Role of the small GTPase Rab27a during Herpes simplex virus infection of oligodendrocytic cells
dc.creator.none.fl_str_mv Bello-Morales Arroyo, Ángeles Raquel
Crespillo, Antonio Jesús
Fraile-Ramos, Alberto
Tabares López, Enrique
Alcina, Antonio
López Guerrero, José Antonio
author Bello-Morales Arroyo, Ángeles Raquel
author_facet Bello-Morales Arroyo, Ángeles Raquel
Crespillo, Antonio Jesús
Fraile-Ramos, Alberto
Tabares López, Enrique
Alcina, Antonio
López Guerrero, José Antonio
author_role author
author2 Crespillo, Antonio Jesús
Fraile-Ramos, Alberto
Tabares López, Enrique
Alcina, Antonio
López Guerrero, José Antonio
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Biología Molecular
Facultad de Ciencias
dc.subject.none.fl_str_mv HSV-1
Morphogenesis
Oligodendrocytes
Rab27a
Tegument
Viral egress
Biología y Biomedicina / Biología
topic HSV-1
Morphogenesis
Oligodendrocytes
Rab27a
Tegument
Viral egress
Biología y Biomedicina / Biología
description Background: The morphogenesis of herpes simplex virus type 1 (HSV-1) comprises several events, of which some are not completely understood. It has been shown that HSV-1 glycoproteins accumulate in the trans-Golgi network (TGN) and in TGN-derived vesicles. It is also accepted that HSV-1 acquires its final morphology through a secondary envelopment by budding into TGN-derived vesicles coated with viral glycoproteins and tegument proteins. Nevertheless, several aspects of this process remain elusive. The small GTPase Rab27a has been implicated in regulated exocytosis, and it seems to play a key role in certain membrane trafficking events. Rab27a also seems to be required for human cytomegalovirus assembly. However, despite the involvement of various Rab GTPases in HSV-1 envelopment, there is, to date, no data reported on the role of Rab27a in HSV-1 infection. Results: Herein, we show that Rab27a colocalized with GHSV-UL46, a tegument-tagged green fluorescent protein-HSV-1, in the TGN. In fact, this small GTPase colocalized with viral glycoproteins gH and gD in that compartment. Functional analysis through Rab27a depletion showed a significant decrease in the number of infected cells and viral production in Rab27a-silenced cells. Conclusions: Altogether, our results indicate that Rab27a plays an important role in HSV-1 infection of oligodendrocytic cells
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-11-21
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/662847
https://dx.doi.org/10.1186/1471-2180-12-265
url http://hdl.handle.net/10486/662847
https://dx.doi.org/10.1186/1471-2180-12-265
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The Authors
publisher.none.fl_str_mv The Authors
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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