Phenotypic profile of expanded NK cells in chronic lymphoproliferative disorders: a surrogate marker for NK-cell clonality
This is an open-access article distributed under the terms of the Creative Commons Attribution License.
| Autores: | , , , , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/135729 |
| Acceso en línea: | http://hdl.handle.net/10261/135729 |
| Access Level: | acceso abierto |
| Palabra clave: | Natural killer cells Clonality Immunophenotype CLPD-NK NK cells |
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Phenotypic profile of expanded NK cells in chronic lymphoproliferative disorders: a surrogate marker for NK-cell clonalityBárcena, PalomaJara-Acevedo, MariaTabernero, María D.López, AntonioSánchez, Maria LuzGarcía-Montero, AndrésMuñoz-García, NoemíVidriales, Maria BelénPaiva, ArturLécrevisse, QuentinLima, MargaridaLangerak, Anton W.Böttcher, SebastianDongen, J. J. M. vanOrfao, AlbertoAlmeida, JuliaNatural killer cellsClonalityImmunophenotypeCLPD-NKNK cellsThis is an open-access article distributed under the terms of the Creative Commons Attribution License.Currently, the lack of a universal and specific marker of clonality hampers the diagnosis and classification of chronic expansions of natural killer (NK) cells. Here we investigated the utility of flow cytometric detection of aberrant/altered NK-cell phenotypes as a surrogate marker for clonality, in the diagnostic work-up of chronic lymphoproliferative disorders of NK cells (CLPD-NK). For this purpose, a large panel of markers was evaluated by multiparametric flow cytometry on peripheral blood (PB) CD56 NK cells from 60 patients, including 23 subjects with predefined clonal (n = 9) and polyclonal (n = 14) CD56 NK-cell expansions, and 37 with CLPD-NK of undetermined clonality; also, PB samples from 10 healthy adults were included. Clonality was established using the human androgen receptor (HUMARA) assay. Clonal NK cells were found to show decreased expression of CD7, CD11b and CD38, and higher CD2, CD94 and HLADR levels vs. normal NK cells, together with a restricted repertoire of expression of the CD158a, CD158b and CD161 killer-associated receptors. In turn, NK cells from both clonal and polyclonal CLPD-NK showed similar/overlapping phenotypic profiles, except for high and more homogeneous expression of CD94 and HLADR, which was restricted to clonal CLPD-NK. We conclude that the CD94/HLADR phenotypic profile proved to be a useful surrogate marker for NK-cell clonality.This work has been partially supported by the following grants: FIS 02/1244-FEDER, DTS 15/00119-FEDER, RTICC RD06/0020/0035-FEDER and RTICC RD12/0036/0048-FEDER from the Fondo de Investigación Sanitaria, Instituto de Salud Carlos III, Ministerio de Economía y Competitividad, Madrid, Spain; SA103/03 and SA079U14 from the Consejería de Educación, Junta de Castilla y León, Valladolid, Spain. The research activities of the EuroFlow Consortium were supported by the European Commission (grant STREP EU-FP6, LSHB-CT-2006–018708, entitled ‘Flow cytometry for fast and sensitive diagnosis and follow-up of hematological malignancies’).Peer ReviewedImpact JournalsInstituto de Salud Carlos IIIEuropean CommissionJunta de Castilla y LeónMinisterio de Economía y Competitividad (España)Instituto de Salud Carlos IIIRed Temática de Investigación Cooperativa en Cáncer (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2016201620152016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/135729reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.18632/oncotarget.5480Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1357292026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
Phenotypic profile of expanded NK cells in chronic lymphoproliferative disorders: a surrogate marker for NK-cell clonality |
| title |
Phenotypic profile of expanded NK cells in chronic lymphoproliferative disorders: a surrogate marker for NK-cell clonality |
| spellingShingle |
Phenotypic profile of expanded NK cells in chronic lymphoproliferative disorders: a surrogate marker for NK-cell clonality Bárcena, Paloma Natural killer cells Clonality Immunophenotype CLPD-NK NK cells |
| title_short |
Phenotypic profile of expanded NK cells in chronic lymphoproliferative disorders: a surrogate marker for NK-cell clonality |
| title_full |
Phenotypic profile of expanded NK cells in chronic lymphoproliferative disorders: a surrogate marker for NK-cell clonality |
| title_fullStr |
Phenotypic profile of expanded NK cells in chronic lymphoproliferative disorders: a surrogate marker for NK-cell clonality |
| title_full_unstemmed |
Phenotypic profile of expanded NK cells in chronic lymphoproliferative disorders: a surrogate marker for NK-cell clonality |
| title_sort |
Phenotypic profile of expanded NK cells in chronic lymphoproliferative disorders: a surrogate marker for NK-cell clonality |
| dc.creator.none.fl_str_mv |
Bárcena, Paloma Jara-Acevedo, Maria Tabernero, María D. López, Antonio Sánchez, Maria Luz García-Montero, Andrés Muñoz-García, Noemí Vidriales, Maria Belén Paiva, Artur Lécrevisse, Quentin Lima, Margarida Langerak, Anton W. Böttcher, Sebastian Dongen, J. J. M. van Orfao, Alberto Almeida, Julia |
| author |
Bárcena, Paloma |
| author_facet |
Bárcena, Paloma Jara-Acevedo, Maria Tabernero, María D. López, Antonio Sánchez, Maria Luz García-Montero, Andrés Muñoz-García, Noemí Vidriales, Maria Belén Paiva, Artur Lécrevisse, Quentin Lima, Margarida Langerak, Anton W. Böttcher, Sebastian Dongen, J. J. M. van Orfao, Alberto Almeida, Julia |
| author_role |
author |
| author2 |
Jara-Acevedo, Maria Tabernero, María D. López, Antonio Sánchez, Maria Luz García-Montero, Andrés Muñoz-García, Noemí Vidriales, Maria Belén Paiva, Artur Lécrevisse, Quentin Lima, Margarida Langerak, Anton W. Böttcher, Sebastian Dongen, J. J. M. van Orfao, Alberto Almeida, Julia |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Instituto de Salud Carlos III European Commission Junta de Castilla y León Ministerio de Economía y Competitividad (España) Instituto de Salud Carlos III Red Temática de Investigación Cooperativa en Cáncer (España) Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Natural killer cells Clonality Immunophenotype CLPD-NK NK cells |
| topic |
Natural killer cells Clonality Immunophenotype CLPD-NK NK cells |
| description |
This is an open-access article distributed under the terms of the Creative Commons Attribution License. |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2015 2016 2016 2016 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/135729 |
| url |
http://hdl.handle.net/10261/135729 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
http://dx.doi.org/10.18632/oncotarget.5480 Sí |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Impact Journals |
| publisher.none.fl_str_mv |
Impact Journals |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
| reponame_str |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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