Analysis of multiple-herbicide resistant Amaranthus palmeri populations from Spain points to an introduction of the eccDNA from America

The herbicide-resistant invasive weed species Amaranthus palmeri threatens agricultural production and native plant ecology in Spain, as well as in other European countries. Understanding whether herbicide resistance alleles evolve in situ or are introduced via gene flow remains unclear. To address...

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Autores: Manicardi, Alfredo, Mora, Germán, Araujo, André Lucas Simões, Gaines, Todd A., Lozano-Juste, Jorge, Torra Farré, Joel
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10459.1/468474
Acceso en línea:https://doi.org/10.1002/ps.70034
https://hdl.handle.net/10459.1/468474
Access Level:acceso abierto
Palabra clave:ALS-resistance
Extrachromosomal circular DNA
Glyphosate-resistance
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network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Analysis of multiple-herbicide resistant Amaranthus palmeri populations from Spain points to an introduction of the eccDNA from America
title Analysis of multiple-herbicide resistant Amaranthus palmeri populations from Spain points to an introduction of the eccDNA from America
spellingShingle Analysis of multiple-herbicide resistant Amaranthus palmeri populations from Spain points to an introduction of the eccDNA from America
Manicardi, Alfredo
ALS-resistance
Extrachromosomal circular DNA
Glyphosate-resistance
title_short Analysis of multiple-herbicide resistant Amaranthus palmeri populations from Spain points to an introduction of the eccDNA from America
title_full Analysis of multiple-herbicide resistant Amaranthus palmeri populations from Spain points to an introduction of the eccDNA from America
title_fullStr Analysis of multiple-herbicide resistant Amaranthus palmeri populations from Spain points to an introduction of the eccDNA from America
title_full_unstemmed Analysis of multiple-herbicide resistant Amaranthus palmeri populations from Spain points to an introduction of the eccDNA from America
title_sort Analysis of multiple-herbicide resistant Amaranthus palmeri populations from Spain points to an introduction of the eccDNA from America
dc.creator.none.fl_str_mv Manicardi, Alfredo
Mora, Germán
Araujo, André Lucas Simões
Gaines, Todd A.
Lozano-Juste, Jorge
Torra Farré, Joel
author Manicardi, Alfredo
author_facet Manicardi, Alfredo
Mora, Germán
Araujo, André Lucas Simões
Gaines, Todd A.
Lozano-Juste, Jorge
Torra Farré, Joel
author_role author
author2 Mora, Germán
Araujo, André Lucas Simões
Gaines, Todd A.
Lozano-Juste, Jorge
Torra Farré, Joel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ALS-resistance
Extrachromosomal circular DNA
Glyphosate-resistance
topic ALS-resistance
Extrachromosomal circular DNA
Glyphosate-resistance
description The herbicide-resistant invasive weed species Amaranthus palmeri threatens agricultural production and native plant ecology in Spain, as well as in other European countries. Understanding whether herbicide resistance alleles evolve in situ or are introduced via gene flow remains unclear. To address this, we characterized multiple resistance to acetolactate synthase (ALS)-- and 5-enolpyruvylshikimate-3phosphate synthase (EPSPS)-inhibiting herbicides in two Spanish A. palmeri populations at the plant level. Additionally, we analyzed the extra-chromosomal circular DNA (eccDNA) to determine whether glyphosate resistance resulted from local selection pressure or was introduced by gene flow. RESULTS: Both populations exhibit individuals that survived both herbicide MoA, with multiple resistance mechanisms to ALS- and EPSPS-inhibiting herbicides. Eight different ALS allele mutations were identified in resistant plants, including Pro-197-Ile, reported only in one species previously. Glyphosate resistance in the two populations is to the result of gene duplication mediated by eccDNA. Spanish and North American eccDNAs showed complete identity, with no single nucleotide polymorphisms (SNPs) found between the partial analyzed sequences of noncoding regions. CONCLUSION: We confirm for the first time in Europe resistance to ALS and EPSPS inhibitors at both the population and individual levels in two Spanish A. palmeri populations. The absence of SNPs in the eccDNA from Spanish populations compared to the reference American sequence and the presence of target-site mutations in the ALS gene occurred without selective pressure from ALS herbicides, suggests that the origin of resistance traits may have evolved elsewhere and been introduced from the place of origin to Spain. However, it is important to note that the limited number of populations studied and the partial sequencing of eccDNA do not provide definitive confirmation of the exact origins of resistance mechanisms. This work raises concerns about the arrival of this and potentially other new herbicide-resistant A. palmeri populations in Europe posing challenges for management.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://doi.org/10.1002/ps.70034
https://hdl.handle.net/10459.1/468474
url https://doi.org/10.1002/ps.70034
https://hdl.handle.net/10459.1/468474
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113229RB-C42
Reproducció del document publicat a https://doi.org/10.1002/ps.70034
Pest Management Science, 2025, vol. 81, núm. 10, p. 6807-6819
info:eu-repo/grantAgreement/EC/H2020/801586
dc.rights.none.fl_str_mv cc-by (c) Manicardi et al., 2025
Attribution 4.0 International
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c) Manicardi et al., 2025
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley and Sons Ltd
publisher.none.fl_str_mv John Wiley and Sons Ltd
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Analysis of multiple-herbicide resistant Amaranthus palmeri populations from Spain points to an introduction of the eccDNA from AmericaManicardi, AlfredoMora, GermánAraujo, André Lucas SimõesGaines, Todd A.Lozano-Juste, JorgeTorra Farré, JoelALS-resistanceExtrachromosomal circular DNAGlyphosate-resistanceThe herbicide-resistant invasive weed species Amaranthus palmeri threatens agricultural production and native plant ecology in Spain, as well as in other European countries. Understanding whether herbicide resistance alleles evolve in situ or are introduced via gene flow remains unclear. To address this, we characterized multiple resistance to acetolactate synthase (ALS)-- and 5-enolpyruvylshikimate-3phosphate synthase (EPSPS)-inhibiting herbicides in two Spanish A. palmeri populations at the plant level. Additionally, we analyzed the extra-chromosomal circular DNA (eccDNA) to determine whether glyphosate resistance resulted from local selection pressure or was introduced by gene flow. RESULTS: Both populations exhibit individuals that survived both herbicide MoA, with multiple resistance mechanisms to ALS- and EPSPS-inhibiting herbicides. Eight different ALS allele mutations were identified in resistant plants, including Pro-197-Ile, reported only in one species previously. Glyphosate resistance in the two populations is to the result of gene duplication mediated by eccDNA. Spanish and North American eccDNAs showed complete identity, with no single nucleotide polymorphisms (SNPs) found between the partial analyzed sequences of noncoding regions. CONCLUSION: We confirm for the first time in Europe resistance to ALS and EPSPS inhibitors at both the population and individual levels in two Spanish A. palmeri populations. The absence of SNPs in the eccDNA from Spanish populations compared to the reference American sequence and the presence of target-site mutations in the ALS gene occurred without selective pressure from ALS herbicides, suggests that the origin of resistance traits may have evolved elsewhere and been introduced from the place of origin to Spain. However, it is important to note that the limited number of populations studied and the partial sequencing of eccDNA do not provide definitive confirmation of the exact origins of resistance mechanisms. This work raises concerns about the arrival of this and potentially other new herbicide-resistant A. palmeri populations in Europe posing challenges for management.We would like to acknowledge Maria Casamitjana Escobedo (University of Lleida) for providing technical support for plant growth. Her contributions were important to the completion of this study. This work has been supported by the Spanish State Research Agency, Spain (AEI) and the European Regional Development Fund, EU (ERDF) through the project PID2020\u2010113229RB\u2010C42. Joel Torra acknowledges support from the Spanish Ministry of Science, Innovation, and Universities. This project has received funding from the European Union's H2020 research and innovation program under Marie Sklodowska\u2010Curie grant agreement No 801586. Work at J.L\u2010J.'s group is funded by Spanish MCIN/AEI/PID2021\u2010128826OAI00, RYC2020\u2010029097\u2010I, (CISEJI/2022/26) grants and the AGROALNEXT program supported by MCIN with funding from European Union NextGenerationEU (PRTR\u2010C17.I1) and by Generalitat Valenciana. This work was conducted under the auspices of the research network PalmerNET funded by the Spanish Ministry of Science and Innovation (RED2022\u2010134285\u2010T).John Wiley and Sons Ltd2025info:eu-repo/semantics/articlehttps://doi.org/10.1002/ps.70034https://hdl.handle.net/10459.1/468474reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113229RB-C42Reproducció del document publicat a https://doi.org/10.1002/ps.70034Pest Management Science, 2025, vol. 81, núm. 10, p. 6807-6819info:eu-repo/grantAgreement/EC/H2020/801586cc-by (c) Manicardi et al., 2025Attribution 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:recercat.cat:10459.1/4684742026-05-29T05:05:01Z
score 15,81155

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