Chromosome instability in mouse embryonic stem cells

Embryonic Stem Cells (ESCs) are expected to show a stable euploid karyotype, but in the last decade (sub)chromosomal aberrations have been systematically described in these cell lines when maintained in vitro. Culture conditions and long-term culture have been traditionally proposed as possible fact...

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Autores: Gaztelumendi, Nerea|||0000-0002-3837-2956, Nogués, Carme|||0000-0002-6361-8559
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:182999
Acceso en línea:https://ddd.uab.cat/record/182999
https://dx.doi.org/urn:doi:10.1038/srep05324
Access Level:acceso abierto
Palabra clave:Chromosomes
Embryonic stem cells
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spelling Chromosome instability in mouse embryonic stem cellsGaztelumendi, Nerea|||0000-0002-3837-2956Nogués, Carme|||0000-0002-6361-8559ChromosomesEmbryonic stem cellsEmbryonic Stem Cells (ESCs) are expected to show a stable euploid karyotype, but in the last decade (sub)chromosomal aberrations have been systematically described in these cell lines when maintained in vitro. Culture conditions and long-term culture have been traditionally proposed as possible factors involved in the acquisition of chromosomal abnormalities. Thus, we analyzed the chromosome constitution, the undifferentiated state and the functional pluripotency of three different mouse ESCs grown under the same culture conditions. Two cell lines were unstable from early passages, whereas the third one retained its chromosome integrity after long-term culture despite using enzymatic methods for cell disaggregation. Trisomy 8 and 11 were clonally selected in both unstable cell lines, which also showed a higher growth rate than our normal cell line and suffered morphological changes in colony shape with increasing passage number. Regardless of the length of culture or the chromosome instability, all cell lines preserved their differentiation potential. These results confirm that double trisomy 8 and 11 confers a growth advantage to the abnormal cells, but not at the expense of cell differentiation. The presence of chromosome instability, widely related to tumor development and cancer disease, highlights the risk of using pluripotent cells in regenerative medicine. 22017-01-0120172017-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/182999https://dx.doi.org/urn:doi:10.1038/srep05324reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 TEC2011-29140-C03-03open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.https://creativecommons.org/licenses/by-nc-nd/3.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1829992026-06-06T12:50:31Z
dc.title.none.fl_str_mv Chromosome instability in mouse embryonic stem cells
title Chromosome instability in mouse embryonic stem cells
spellingShingle Chromosome instability in mouse embryonic stem cells
Gaztelumendi, Nerea|||0000-0002-3837-2956
Chromosomes
Embryonic stem cells
title_short Chromosome instability in mouse embryonic stem cells
title_full Chromosome instability in mouse embryonic stem cells
title_fullStr Chromosome instability in mouse embryonic stem cells
title_full_unstemmed Chromosome instability in mouse embryonic stem cells
title_sort Chromosome instability in mouse embryonic stem cells
dc.creator.none.fl_str_mv Gaztelumendi, Nerea|||0000-0002-3837-2956
Nogués, Carme|||0000-0002-6361-8559
author Gaztelumendi, Nerea|||0000-0002-3837-2956
author_facet Gaztelumendi, Nerea|||0000-0002-3837-2956
Nogués, Carme|||0000-0002-6361-8559
author_role author
author2 Nogués, Carme|||0000-0002-6361-8559
author2_role author
dc.subject.none.fl_str_mv Chromosomes
Embryonic stem cells
topic Chromosomes
Embryonic stem cells
description Embryonic Stem Cells (ESCs) are expected to show a stable euploid karyotype, but in the last decade (sub)chromosomal aberrations have been systematically described in these cell lines when maintained in vitro. Culture conditions and long-term culture have been traditionally proposed as possible factors involved in the acquisition of chromosomal abnormalities. Thus, we analyzed the chromosome constitution, the undifferentiated state and the functional pluripotency of three different mouse ESCs grown under the same culture conditions. Two cell lines were unstable from early passages, whereas the third one retained its chromosome integrity after long-term culture despite using enzymatic methods for cell disaggregation. Trisomy 8 and 11 were clonally selected in both unstable cell lines, which also showed a higher growth rate than our normal cell line and suffered morphological changes in colony shape with increasing passage number. Regardless of the length of culture or the chromosome instability, all cell lines preserved their differentiation potential. These results confirm that double trisomy 8 and 11 confers a growth advantage to the abnormal cells, but not at the expense of cell differentiation. The presence of chromosome instability, widely related to tumor development and cancer disease, highlights the risk of using pluripotent cells in regenerative medicine.
publishDate 2017
dc.date.none.fl_str_mv 2
2017-01-01
2017
2017-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/182999
https://dx.doi.org/urn:doi:10.1038/srep05324
url https://ddd.uab.cat/record/182999
https://dx.doi.org/urn:doi:10.1038/srep05324
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 TEC2011-29140-C03-03
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/3.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/3.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
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