Protein Biosynthesis and Carbon Catabolite Repression Are Transcriptionally Upregulated in Saccharomyces cerevisiae by Extracellular Fractions From Several Wine Yeast Species

Non-Saccharomyces yeast species are increasingly used in winemaking in combination with Saccharomyces cerevisiae to modulate sensory attributes or as processing aids. Consequently, there is academic and practical interest in understanding how different yeast species interact with each other in grape...

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Detalles Bibliográficos
Autores: Mejías-Ortiz, Miguel, Morales, Pilar, Juárez-Fernández, Guillermo, González García, Ramón
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/400402
Acceso en línea:http://hdl.handle.net/10261/400402
https://api.elsevier.com/content/abstract/scopus_id/105006589073
Access Level:acceso abierto
Palabra clave:Extracellular vesicles
Microbial communication
Non‐Saccharomyces
Wine
Yeast interactions
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spelling Protein Biosynthesis and Carbon Catabolite Repression Are Transcriptionally Upregulated in Saccharomyces cerevisiae by Extracellular Fractions From Several Wine Yeast SpeciesMejías-Ortiz, MiguelMorales, PilarJuárez-Fernández, GuillermoGonzález García, RamónExtracellular vesiclesMicrobial communicationNon‐SaccharomycesWineYeast interactionsNon-Saccharomyces yeast species are increasingly used in winemaking in combination with Saccharomyces cerevisiae to modulate sensory attributes or as processing aids. Consequently, there is academic and practical interest in understanding how different yeast species interact with each other in grape must. Although interactions will depend on the metabolic capabilities of the strains involved, there are other possible interaction mechanisms between wine yeasts. In this work we used extracellular vesicle (EV)-enriched fractions from different non-Saccharomyces species to challenge S. cerevisiae inoculated in synthetic grape must. The results show that the previously described response to EVs of Metschnikowia pulcherrima was not an isolated phenomenon, but that S. cerevisiae responds in a general way to EVs of other yeast species. Meta-analysis of the results points to protein biosynthesis and carbon catabolite repression as general targets; both being stimulated by the interaction, beyond the acclimatisation to the synthetic juice experienced by the control cells. The intensity of the response showed differences between the four species; while the transcriptional response to M. pulcherrima EVs clearly diverges from that to EVs of the other yeast species, which show greater similarity to each other.This work was supported by the Spanish Government through grant PID2022-136815OB-I00 funded by MCIN/AEI/ 10.13039/501100011033, and grant PRE2020-093420 funded by MCIN/AEI/ 10.13039/501100011033 and by ‘ESF Investing in your future’ (training contract for MM).Peer reviewedJohn Wiley & SonsAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)European CommissionMejías-Ortiz, Miguel [0000-0001-7065-4331]González García, Ramón [0000-0001-7388-1660]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/400402https://api.elsevier.com/content/abstract/scopus_id/105006589073reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI//PID2022-136815OB-I00The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1111/1751-7915.70168https://doi.org/10.1111/1751-7915.70168Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4004022026-05-22T06:33:51Z
dc.title.none.fl_str_mv Protein Biosynthesis and Carbon Catabolite Repression Are Transcriptionally Upregulated in Saccharomyces cerevisiae by Extracellular Fractions From Several Wine Yeast Species
title Protein Biosynthesis and Carbon Catabolite Repression Are Transcriptionally Upregulated in Saccharomyces cerevisiae by Extracellular Fractions From Several Wine Yeast Species
spellingShingle Protein Biosynthesis and Carbon Catabolite Repression Are Transcriptionally Upregulated in Saccharomyces cerevisiae by Extracellular Fractions From Several Wine Yeast Species
Mejías-Ortiz, Miguel
Extracellular vesicles
Microbial communication
Non‐Saccharomyces
Wine
Yeast interactions
title_short Protein Biosynthesis and Carbon Catabolite Repression Are Transcriptionally Upregulated in Saccharomyces cerevisiae by Extracellular Fractions From Several Wine Yeast Species
title_full Protein Biosynthesis and Carbon Catabolite Repression Are Transcriptionally Upregulated in Saccharomyces cerevisiae by Extracellular Fractions From Several Wine Yeast Species
title_fullStr Protein Biosynthesis and Carbon Catabolite Repression Are Transcriptionally Upregulated in Saccharomyces cerevisiae by Extracellular Fractions From Several Wine Yeast Species
title_full_unstemmed Protein Biosynthesis and Carbon Catabolite Repression Are Transcriptionally Upregulated in Saccharomyces cerevisiae by Extracellular Fractions From Several Wine Yeast Species
title_sort Protein Biosynthesis and Carbon Catabolite Repression Are Transcriptionally Upregulated in Saccharomyces cerevisiae by Extracellular Fractions From Several Wine Yeast Species
dc.creator.none.fl_str_mv Mejías-Ortiz, Miguel
Morales, Pilar
Juárez-Fernández, Guillermo
González García, Ramón
author Mejías-Ortiz, Miguel
author_facet Mejías-Ortiz, Miguel
Morales, Pilar
Juárez-Fernández, Guillermo
González García, Ramón
author_role author
author2 Morales, Pilar
Juárez-Fernández, Guillermo
González García, Ramón
author2_role author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
European Commission
Mejías-Ortiz, Miguel [0000-0001-7065-4331]
González García, Ramón [0000-0001-7388-1660]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Extracellular vesicles
Microbial communication
Non‐Saccharomyces
Wine
Yeast interactions
topic Extracellular vesicles
Microbial communication
Non‐Saccharomyces
Wine
Yeast interactions
description Non-Saccharomyces yeast species are increasingly used in winemaking in combination with Saccharomyces cerevisiae to modulate sensory attributes or as processing aids. Consequently, there is academic and practical interest in understanding how different yeast species interact with each other in grape must. Although interactions will depend on the metabolic capabilities of the strains involved, there are other possible interaction mechanisms between wine yeasts. In this work we used extracellular vesicle (EV)-enriched fractions from different non-Saccharomyces species to challenge S. cerevisiae inoculated in synthetic grape must. The results show that the previously described response to EVs of Metschnikowia pulcherrima was not an isolated phenomenon, but that S. cerevisiae responds in a general way to EVs of other yeast species. Meta-analysis of the results points to protein biosynthesis and carbon catabolite repression as general targets; both being stimulated by the interaction, beyond the acclimatisation to the synthetic juice experienced by the control cells. The intensity of the response showed differences between the four species; while the transcriptional response to M. pulcherrima EVs clearly diverges from that to EVs of the other yeast species, which show greater similarity to each other.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/400402
https://api.elsevier.com/content/abstract/scopus_id/105006589073
url http://hdl.handle.net/10261/400402
https://api.elsevier.com/content/abstract/scopus_id/105006589073
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI//PID2022-136815OB-I00
The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1111/1751-7915.70168
https://doi.org/10.1111/1751-7915.70168
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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