The role of Xpert MTB/RIF in diagnosing pulmonary tuberculosis in post-mortem tissues

The extent to which the Xpert MTB/RIF (Gene Xpert) contributes to tuberculosis (TB) diagnosis in samples other than sputum and cerebrospinal fluid remains uncertain. We aimed to assess the role of Xpert MTB/RIF for detecting M. tuberculosis in post-mortem tissues. We conducted a study among 30 compl...

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Autores: García-Basteiro, Alberto L., Ismail, Mamudo Rafik, Carrilho, Carla, Ussene, Esperança, Castillo, Paola, Chitsungo, Dércio, Rodríguez, Cristina, Lovane, Lucilia, Vergara Gómez, Andrea, López Varela, Elisa, Mandomando, Inácio, Lorenzoni, Cesaltina, Ordi i Majà, Jaume, Menéndez, Clara, Bassat Orellana, Quique, Martínez Yoldi, Miguel Julián
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/96870
Acceso en línea:https://hdl.handle.net/2445/96870
Access Level:acceso abierto
Palabra clave:Bacteriologia
Microbiologia mèdica
Malalties infeccioses
Bacteriology
Medical microbiology
Communicable diseases
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spelling The role of Xpert MTB/RIF in diagnosing pulmonary tuberculosis in post-mortem tissuesGarcía-Basteiro, Alberto L.Ismail, Mamudo RafikCarrilho, CarlaUssene, EsperançaCastillo, PaolaChitsungo, DércioRodríguez, CristinaLovane, LuciliaVergara Gómez, AndreaLópez Varela, ElisaMandomando, InácioLorenzoni, CesaltinaOrdi i Majà, JaumeMenéndez, ClaraBassat Orellana, QuiqueMartínez Yoldi, Miguel JuliánBacteriologiaMicrobiologia mèdicaMalalties infecciosesBacteriologyMedical microbiologyCommunicable diseasesThe extent to which the Xpert MTB/RIF (Gene Xpert) contributes to tuberculosis (TB) diagnosis in samples other than sputum and cerebrospinal fluid remains uncertain. We aimed to assess the role of Xpert MTB/RIF for detecting M. tuberculosis in post-mortem tissues. We conducted a study among 30 complete diagnostic autopsies (CDA) performed at the Maputo Central Hospital (Mozambique). Lung tissues were screened for TB in all cases. In addition other tissues were tested when compatible lesions were identified in the histological exam. We used in-house real time PCR and LAMP assays to confirm the presence of M. tuberculosis DNA. The diagnosis of tuberculosis at death was established based on microbiological and histopathological results. Eight out of 30 cases (26.7%) were diagnosed of tuberculosis. Xpert had a sensitivity to detect TB in lung tissue of 87.5% (95% CI 47.3-99.7) and a specificity of 95.7% (95% CI: 78.1-99.9). In-house DNA amplification methods and Xpert showed 93.6% concordance for lung tissue and 100% concordance for brain and liver tissues. The final cause of death was attributable to tuberculosis in four cases. Xpert MTB/RIF may represent a valuable, easy-to perform technique for post-mortem TB diagnosis.Nature Publishing Group2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/96870Articles publicats en revistes (Fonaments Clínics)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1038/srep20703Scientific Reports, 2016, vol. 6, num. 20703http://dx.doi.org/10.1038/srep20703cc by (c) Garcia-Basteiro et al., 2016http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/968702026-05-27T06:46:51Z
dc.title.none.fl_str_mv The role of Xpert MTB/RIF in diagnosing pulmonary tuberculosis in post-mortem tissues
title The role of Xpert MTB/RIF in diagnosing pulmonary tuberculosis in post-mortem tissues
spellingShingle The role of Xpert MTB/RIF in diagnosing pulmonary tuberculosis in post-mortem tissues
García-Basteiro, Alberto L.
Bacteriologia
Microbiologia mèdica
Malalties infeccioses
Bacteriology
Medical microbiology
Communicable diseases
title_short The role of Xpert MTB/RIF in diagnosing pulmonary tuberculosis in post-mortem tissues
title_full The role of Xpert MTB/RIF in diagnosing pulmonary tuberculosis in post-mortem tissues
title_fullStr The role of Xpert MTB/RIF in diagnosing pulmonary tuberculosis in post-mortem tissues
title_full_unstemmed The role of Xpert MTB/RIF in diagnosing pulmonary tuberculosis in post-mortem tissues
title_sort The role of Xpert MTB/RIF in diagnosing pulmonary tuberculosis in post-mortem tissues
dc.creator.none.fl_str_mv García-Basteiro, Alberto L.
Ismail, Mamudo Rafik
Carrilho, Carla
Ussene, Esperança
Castillo, Paola
Chitsungo, Dércio
Rodríguez, Cristina
Lovane, Lucilia
Vergara Gómez, Andrea
López Varela, Elisa
Mandomando, Inácio
Lorenzoni, Cesaltina
Ordi i Majà, Jaume
Menéndez, Clara
Bassat Orellana, Quique
Martínez Yoldi, Miguel Julián
author García-Basteiro, Alberto L.
author_facet García-Basteiro, Alberto L.
Ismail, Mamudo Rafik
Carrilho, Carla
Ussene, Esperança
Castillo, Paola
Chitsungo, Dércio
Rodríguez, Cristina
Lovane, Lucilia
Vergara Gómez, Andrea
López Varela, Elisa
Mandomando, Inácio
Lorenzoni, Cesaltina
Ordi i Majà, Jaume
Menéndez, Clara
Bassat Orellana, Quique
Martínez Yoldi, Miguel Julián
author_role author
author2 Ismail, Mamudo Rafik
Carrilho, Carla
Ussene, Esperança
Castillo, Paola
Chitsungo, Dércio
Rodríguez, Cristina
Lovane, Lucilia
Vergara Gómez, Andrea
López Varela, Elisa
Mandomando, Inácio
Lorenzoni, Cesaltina
Ordi i Majà, Jaume
Menéndez, Clara
Bassat Orellana, Quique
Martínez Yoldi, Miguel Julián
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bacteriologia
Microbiologia mèdica
Malalties infeccioses
Bacteriology
Medical microbiology
Communicable diseases
topic Bacteriologia
Microbiologia mèdica
Malalties infeccioses
Bacteriology
Medical microbiology
Communicable diseases
description The extent to which the Xpert MTB/RIF (Gene Xpert) contributes to tuberculosis (TB) diagnosis in samples other than sputum and cerebrospinal fluid remains uncertain. We aimed to assess the role of Xpert MTB/RIF for detecting M. tuberculosis in post-mortem tissues. We conducted a study among 30 complete diagnostic autopsies (CDA) performed at the Maputo Central Hospital (Mozambique). Lung tissues were screened for TB in all cases. In addition other tissues were tested when compatible lesions were identified in the histological exam. We used in-house real time PCR and LAMP assays to confirm the presence of M. tuberculosis DNA. The diagnosis of tuberculosis at death was established based on microbiological and histopathological results. Eight out of 30 cases (26.7%) were diagnosed of tuberculosis. Xpert had a sensitivity to detect TB in lung tissue of 87.5% (95% CI 47.3-99.7) and a specificity of 95.7% (95% CI: 78.1-99.9). In-house DNA amplification methods and Xpert showed 93.6% concordance for lung tissue and 100% concordance for brain and liver tissues. The final cause of death was attributable to tuberculosis in four cases. Xpert MTB/RIF may represent a valuable, easy-to perform technique for post-mortem TB diagnosis.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/96870
url https://hdl.handle.net/2445/96870
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1038/srep20703
Scientific Reports, 2016, vol. 6, num. 20703
http://dx.doi.org/10.1038/srep20703
dc.rights.none.fl_str_mv cc by (c) Garcia-Basteiro et al., 2016
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Garcia-Basteiro et al., 2016
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Articles publicats en revistes (Fonaments Clínics)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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