CSDE1 intracellular distribution as a biomarker of melanoma prognosis

RNA-binding proteins are emerging as critical modulators of oncogenic cell transformation, malignancy and therapy resistance. We have previously found that the RNA-binding protein Cold Shock Domain containing protein E1 (CSDE1) promotes invasion and metastasis of melanoma, the deadliest form of skin...

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Autores: Indacochea Cusirramos, Alberto, Guitart, Tanit, Boada, Aram, Peg, Vicente, Quer, Ariadna, Laayouni, Hafid, 1968-, Condal, Laura, Espinosa, Pablo, Manzano Mozo, José Luis, Gebauer, Fátima
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Universitat Pompeu Fabra
Repositorio:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/60849
Acceso en línea:http://hdl.handle.net/10230/60849
http://dx.doi.org/10.3390/ijms25042319
Access Level:acceso abierto
Palabra clave:CSDE1
RNA-binding protein
Biomarker
Cytoplasmic–nuclear ratio
Melanoma
Prognosis
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spelling CSDE1 intracellular distribution as a biomarker of melanoma prognosisIndacochea Cusirramos, AlbertoGuitart, TanitBoada, AramPeg, VicenteQuer, AriadnaLaayouni, Hafid, 1968-Condal, LauraEspinosa, PabloManzano Mozo, José LuisGebauer, FátimaCSDE1RNA-binding proteinBiomarkerCytoplasmic–nuclear ratioMelanomaPrognosisRNA-binding proteins are emerging as critical modulators of oncogenic cell transformation, malignancy and therapy resistance. We have previously found that the RNA-binding protein Cold Shock Domain containing protein E1 (CSDE1) promotes invasion and metastasis of melanoma, the deadliest form of skin cancer and also a highly heterogeneous disease in need of predictive biomarkers and druggable targets. Here, we design a monoclonal antibody useful for IHC in the clinical setting and use it to evaluate the prognosis potential of CSDE1 in an exploratory cohort of 149 whole tissue sections including benign nevi and primary tumors and metastasis from melanoma patients. Contrary to expectations for an oncoprotein, we observed a global decrease in CSDE1 levels with increasing malignancy. However, the CSDE1 cytoplasmic/nuclear ratio exhibited a positive correlation with adverse clinical features of primary tumors and emerged as a robust indicator of progression free survival in cutaneous melanoma, highlighting the potential of CSDE1 as a biomarker of prognosis. Our findings provide a novel feature for prognosis assessment and highlight the intricacies of RNA-binding protein dynamics in cancer progression.A.I. and P.E. were supported by PhD4MD fellowships from the CRG and the Emerald program (Marie Skłodowska-Curie grant agreement 101034290), respectively. This work was supported by the following grants to F.G.: PGC2018-099697-B-I00 and PID2021-127948NB-I00 from the Spanish Ministry of Science and Innovation (MCIN) funded by MCIN/ AEI /10.13039/501100011033/ and by ERDF; “la Caixa” Foundation (ID 100010434) under the Grant LCF/PR/HR17/52150016; the Catalan Agency for Research and Universities (SGR-Cat-2021-01215) and intramural funds from the CRG on emergent translational research. We acknowledge the support of the Spanish Ministry of Science and Innovation through the Centro de Excelencia Severo Ochoa (CEX2020-001049-S, MCIN/AEI /10.13039/501100011033) and the Generalitat de Catalunya through the CERCA programme.MDPI202420242024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/60849http://dx.doi.org/10.3390/ijms25042319reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésInt J Mol Sci. 2024 Feb 15;25(4):2319info:eu-repo/grantAgreement/EC/H2020/101034290info:eu-repo/grantAgreement/ES/2PE/PGC2018-099697-B-I00info:eu-repo/grantAgreement/ES/3PE/PID2021-127948NB-I00info:eu-repo/grantAgreement/ES/2PE/CEX2020-001049-S© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/608492026-06-12T07:21:37Z
dc.title.none.fl_str_mv CSDE1 intracellular distribution as a biomarker of melanoma prognosis
title CSDE1 intracellular distribution as a biomarker of melanoma prognosis
spellingShingle CSDE1 intracellular distribution as a biomarker of melanoma prognosis
Indacochea Cusirramos, Alberto
CSDE1
RNA-binding protein
Biomarker
Cytoplasmic–nuclear ratio
Melanoma
Prognosis
title_short CSDE1 intracellular distribution as a biomarker of melanoma prognosis
title_full CSDE1 intracellular distribution as a biomarker of melanoma prognosis
title_fullStr CSDE1 intracellular distribution as a biomarker of melanoma prognosis
title_full_unstemmed CSDE1 intracellular distribution as a biomarker of melanoma prognosis
title_sort CSDE1 intracellular distribution as a biomarker of melanoma prognosis
dc.creator.none.fl_str_mv Indacochea Cusirramos, Alberto
Guitart, Tanit
Boada, Aram
Peg, Vicente
Quer, Ariadna
Laayouni, Hafid, 1968-
Condal, Laura
Espinosa, Pablo
Manzano Mozo, José Luis
Gebauer, Fátima
author Indacochea Cusirramos, Alberto
author_facet Indacochea Cusirramos, Alberto
Guitart, Tanit
Boada, Aram
Peg, Vicente
Quer, Ariadna
Laayouni, Hafid, 1968-
Condal, Laura
Espinosa, Pablo
Manzano Mozo, José Luis
Gebauer, Fátima
author_role author
author2 Guitart, Tanit
Boada, Aram
Peg, Vicente
Quer, Ariadna
Laayouni, Hafid, 1968-
Condal, Laura
Espinosa, Pablo
Manzano Mozo, José Luis
Gebauer, Fátima
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CSDE1
RNA-binding protein
Biomarker
Cytoplasmic–nuclear ratio
Melanoma
Prognosis
topic CSDE1
RNA-binding protein
Biomarker
Cytoplasmic–nuclear ratio
Melanoma
Prognosis
description RNA-binding proteins are emerging as critical modulators of oncogenic cell transformation, malignancy and therapy resistance. We have previously found that the RNA-binding protein Cold Shock Domain containing protein E1 (CSDE1) promotes invasion and metastasis of melanoma, the deadliest form of skin cancer and also a highly heterogeneous disease in need of predictive biomarkers and druggable targets. Here, we design a monoclonal antibody useful for IHC in the clinical setting and use it to evaluate the prognosis potential of CSDE1 in an exploratory cohort of 149 whole tissue sections including benign nevi and primary tumors and metastasis from melanoma patients. Contrary to expectations for an oncoprotein, we observed a global decrease in CSDE1 levels with increasing malignancy. However, the CSDE1 cytoplasmic/nuclear ratio exhibited a positive correlation with adverse clinical features of primary tumors and emerged as a robust indicator of progression free survival in cutaneous melanoma, highlighting the potential of CSDE1 as a biomarker of prognosis. Our findings provide a novel feature for prognosis assessment and highlight the intricacies of RNA-binding protein dynamics in cancer progression.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/60849
http://dx.doi.org/10.3390/ijms25042319
url http://hdl.handle.net/10230/60849
http://dx.doi.org/10.3390/ijms25042319
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Int J Mol Sci. 2024 Feb 15;25(4):2319
info:eu-repo/grantAgreement/EC/H2020/101034290
info:eu-repo/grantAgreement/ES/2PE/PGC2018-099697-B-I00
info:eu-repo/grantAgreement/ES/3PE/PID2021-127948NB-I00
info:eu-repo/grantAgreement/ES/2PE/CEX2020-001049-S
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
collection Repositorio Digital de la UPF
repository.name.fl_str_mv
repository.mail.fl_str_mv
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