Double stranded sperm DNA breaks, measured by comet assay, are associated with unexplained recurrent miscarriage in couples without a female factor

It is known that sperm samples from recurrent pregnancy loss (RPL) couples have an increase in their sperm DNA fragmentation (SDF), but no studies have been performed in order to identify differences between single stranded SDF (ssSDF) and double stranded SDF (dsSDF) in these patients. This could be...

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Autores: Ribas-Maynou, Jordi|||0000-0002-9101-2044, García-Peiró, Agustín|||0000-0002-8492-3165, Fernandez Encinas, Alba|||0000-0003-2701-4739, Amengual Guedan, Maria Jose|||0000-0002-5385-3061, Prada, Elena, Cortés Garmendia, M. Pilar|||0000-0002-5924-0653, Navarro i Ferreté, Joaquima|||0000-0002-9245-2260, Benet i Català, Jordi|||0000-0001-6185-2555
Tipo de documento: artigo
Data de publicação:2012
País:España
Recursos:Universitat Autònoma de Barcelona
Repositório:Dipòsit Digital de Documents de la UAB
Idioma:inglês
OAI Identifier:oai:ddd.uab.cat:108794
Acesso em linha:https://ddd.uab.cat/record/108794
https://dx.doi.org/urn:doi:10.1371/journal.pone.0044679
Access Level:Acceso aberto
Palavra-chave:Avortament espontani
DNA
Abortos de repetición
Recurrent pregnancy loss
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spelling Double stranded sperm DNA breaks, measured by comet assay, are associated with unexplained recurrent miscarriage in couples without a female factorRibas-Maynou, Jordi|||0000-0002-9101-2044García-Peiró, Agustín|||0000-0002-8492-3165Fernandez Encinas, Alba|||0000-0003-2701-4739Amengual Guedan, Maria Jose|||0000-0002-5385-3061Prada, ElenaCortés Garmendia, M. Pilar|||0000-0002-5924-0653Navarro i Ferreté, Joaquima|||0000-0002-9245-2260Benet i Català, Jordi|||0000-0001-6185-2555Avortament espontaniDNAAbortos de repeticiónRecurrent pregnancy lossIt is known that sperm samples from recurrent pregnancy loss (RPL) couples have an increase in their sperm DNA fragmentation (SDF), but no studies have been performed in order to identify differences between single stranded SDF (ssSDF) and double stranded SDF (dsSDF) in these patients. This could be relevant because the type of DNA damage could have different effects. Semen samples were classified attending their clinical status: 25 fertile donors and 20 RPL patients with at least two unexplained first trimester miscarriages. SDF was analysed using alkaline and neutral Comet assay, SCD test and pulsed-field gel electrophoresis (PFGE), and ROC analysis including data from 105 more infertile patients (n = 150) was performed to establish predictive threshold values. SDF for alkaline and neutral Comet, and the SCD test was analysed in these categories of individuals. Data revealed the presence of two subgroups within fertile donors. The values obtained were 21.10±9.13, 23.35±10.45 and 12.31±4.31, respectively, for fertile donors with low values for both ssSDF and dsSDF; 27.86±12.64, 80.69±12.67 and 12.43±5.22, for fertile donors with low ssSDF and high dsSDF; and 33.61±15.50, 84.64±11.28 and 19.28±6.05, for unexplained RPL patients, also showing a low ssSDF and high dsSDF profile. This latter profile was seen in 85% of unexplained RPL and 33% of fertile donors, suggesting that it may be associated to a male risk factor for undergoing RPL. ROC analysis regarding recurrent miscarriage set the cut-off value at 77.50% of dsDNA SDF. PFGE for low ssSDF and high dsSDF profile samples and positive controls treated with DNase, to induce dsDNA breaks, showed a more intense band of about 48 kb, which fits the toroid model of DNA compaction in sperm, pointing out that some nuclease activity may be affecting their sperm DNA in RPL patients. This work identifies a very specific SDF profile related to the paternal risk of having RPL. 22012-01-0120122012-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/108794https://dx.doi.org/urn:doi:10.1371/journal.pone.0044679reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/2.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1087942026-06-06T12:50:31Z
dc.title.none.fl_str_mv Double stranded sperm DNA breaks, measured by comet assay, are associated with unexplained recurrent miscarriage in couples without a female factor
title Double stranded sperm DNA breaks, measured by comet assay, are associated with unexplained recurrent miscarriage in couples without a female factor
spellingShingle Double stranded sperm DNA breaks, measured by comet assay, are associated with unexplained recurrent miscarriage in couples without a female factor
Ribas-Maynou, Jordi|||0000-0002-9101-2044
Avortament espontani
DNA
Abortos de repetición
Recurrent pregnancy loss
title_short Double stranded sperm DNA breaks, measured by comet assay, are associated with unexplained recurrent miscarriage in couples without a female factor
title_full Double stranded sperm DNA breaks, measured by comet assay, are associated with unexplained recurrent miscarriage in couples without a female factor
title_fullStr Double stranded sperm DNA breaks, measured by comet assay, are associated with unexplained recurrent miscarriage in couples without a female factor
title_full_unstemmed Double stranded sperm DNA breaks, measured by comet assay, are associated with unexplained recurrent miscarriage in couples without a female factor
title_sort Double stranded sperm DNA breaks, measured by comet assay, are associated with unexplained recurrent miscarriage in couples without a female factor
dc.creator.none.fl_str_mv Ribas-Maynou, Jordi|||0000-0002-9101-2044
García-Peiró, Agustín|||0000-0002-8492-3165
Fernandez Encinas, Alba|||0000-0003-2701-4739
Amengual Guedan, Maria Jose|||0000-0002-5385-3061
Prada, Elena
Cortés Garmendia, M. Pilar|||0000-0002-5924-0653
Navarro i Ferreté, Joaquima|||0000-0002-9245-2260
Benet i Català, Jordi|||0000-0001-6185-2555
author Ribas-Maynou, Jordi|||0000-0002-9101-2044
author_facet Ribas-Maynou, Jordi|||0000-0002-9101-2044
García-Peiró, Agustín|||0000-0002-8492-3165
Fernandez Encinas, Alba|||0000-0003-2701-4739
Amengual Guedan, Maria Jose|||0000-0002-5385-3061
Prada, Elena
Cortés Garmendia, M. Pilar|||0000-0002-5924-0653
Navarro i Ferreté, Joaquima|||0000-0002-9245-2260
Benet i Català, Jordi|||0000-0001-6185-2555
author_role author
author2 García-Peiró, Agustín|||0000-0002-8492-3165
Fernandez Encinas, Alba|||0000-0003-2701-4739
Amengual Guedan, Maria Jose|||0000-0002-5385-3061
Prada, Elena
Cortés Garmendia, M. Pilar|||0000-0002-5924-0653
Navarro i Ferreté, Joaquima|||0000-0002-9245-2260
Benet i Català, Jordi|||0000-0001-6185-2555
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Avortament espontani
DNA
Abortos de repetición
Recurrent pregnancy loss
topic Avortament espontani
DNA
Abortos de repetición
Recurrent pregnancy loss
description It is known that sperm samples from recurrent pregnancy loss (RPL) couples have an increase in their sperm DNA fragmentation (SDF), but no studies have been performed in order to identify differences between single stranded SDF (ssSDF) and double stranded SDF (dsSDF) in these patients. This could be relevant because the type of DNA damage could have different effects. Semen samples were classified attending their clinical status: 25 fertile donors and 20 RPL patients with at least two unexplained first trimester miscarriages. SDF was analysed using alkaline and neutral Comet assay, SCD test and pulsed-field gel electrophoresis (PFGE), and ROC analysis including data from 105 more infertile patients (n = 150) was performed to establish predictive threshold values. SDF for alkaline and neutral Comet, and the SCD test was analysed in these categories of individuals. Data revealed the presence of two subgroups within fertile donors. The values obtained were 21.10±9.13, 23.35±10.45 and 12.31±4.31, respectively, for fertile donors with low values for both ssSDF and dsSDF; 27.86±12.64, 80.69±12.67 and 12.43±5.22, for fertile donors with low ssSDF and high dsSDF; and 33.61±15.50, 84.64±11.28 and 19.28±6.05, for unexplained RPL patients, also showing a low ssSDF and high dsSDF profile. This latter profile was seen in 85% of unexplained RPL and 33% of fertile donors, suggesting that it may be associated to a male risk factor for undergoing RPL. ROC analysis regarding recurrent miscarriage set the cut-off value at 77.50% of dsDNA SDF. PFGE for low ssSDF and high dsSDF profile samples and positive controls treated with DNase, to induce dsDNA breaks, showed a more intense band of about 48 kb, which fits the toroid model of DNA compaction in sperm, pointing out that some nuclease activity may be affecting their sperm DNA in RPL patients. This work identifies a very specific SDF profile related to the paternal risk of having RPL.
publishDate 2012
dc.date.none.fl_str_mv 2
2012-01-01
2012
2012-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/108794
https://dx.doi.org/urn:doi:10.1371/journal.pone.0044679
url https://ddd.uab.cat/record/108794
https://dx.doi.org/urn:doi:10.1371/journal.pone.0044679
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/2.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/2.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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