Persistence of tracer in the application site -a potential confounding factor in nerve regeneration studies

Selective reinnervation of peripheral targets after nerve injury might be assessed by injecting a first tracer in a target before nerve injury to label the original neuronal population, and applying a second tracer after the regeneration period to label the regenerated population. However, altered u...

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Detalles Bibliográficos
Autores: Puigdellívol Sánchez, Anna, Prats Galino, Alberto, Ruano Gil, Domingo, Molander, Carl
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2003
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/56405
Acceso en línea:https://hdl.handle.net/2445/56405
Access Level:acceso abierto
Palabra clave:Nervis perifèrics
Regeneració del sistema nerviós
Experimentació animal
Peripheral nerves
Nervous system regeneration
Animal experimentation
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spelling Persistence of tracer in the application site -a potential confounding factor in nerve regeneration studiesPuigdellívol Sánchez, AnnaPrats Galino, AlbertoRuano Gil, DomingoMolander, CarlNervis perifèricsRegeneració del sistema nerviósExperimentació animalPeripheral nervesNervous system regenerationAnimal experimentationSelective reinnervation of peripheral targets after nerve injury might be assessed by injecting a first tracer in a target before nerve injury to label the original neuronal population, and applying a second tracer after the regeneration period to label the regenerated population. However, altered uptake of tracer, fading, and cell death may interfere with the results. Furthermore, if the first tracer injected remains in the target tissue, available for 're-uptake' by misdirected regenerating axons, which originally innervated another region, then the identification of the original population would be confused. With the aim of studying this problem, the sciatic nerve of adult rats was sectioned and sutured. After 3 days, to allow the distal axon to degenerate avoiding immediate retrograde transport, one of the dyes: Fast Blue (FB), Fluoro-Gold (FG) or Diamidino Yellow (DY), was injected into the tibial branch of the sciatic nerve, or in the skin of one of the denervated digits. Rats survived 2-3 months. The results showed labelled dorsal root ganglion (DRG) cells and motoneurones, indicating that late re-uptake of a first tracer occurs. This phenomenon must be considered when the model of sequential labelling is used for studying the accuracy of peripheral reinnervation.Elsevier B.V.2014201420032014info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion18 p.application/pdfhttps://hdl.handle.net/2445/56405Articles publicats en revistes (Fonaments Clínics)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: http://dx.doi.org/10.1016/S0165-0270(03)00128-6Journal of Neuroscience Methods, 2003, vol. 127, num. 1, p. 105 -110http://dx.doi.org/10.1016/S0165-0270(03)00128-6(c) Elsevier B.V., 2003info:eu-repo/semantics/openAccessoai:recercat.cat:2445/564052026-05-29T05:05:01Z
dc.title.none.fl_str_mv Persistence of tracer in the application site -a potential confounding factor in nerve regeneration studies
title Persistence of tracer in the application site -a potential confounding factor in nerve regeneration studies
spellingShingle Persistence of tracer in the application site -a potential confounding factor in nerve regeneration studies
Puigdellívol Sánchez, Anna
Nervis perifèrics
Regeneració del sistema nerviós
Experimentació animal
Peripheral nerves
Nervous system regeneration
Animal experimentation
title_short Persistence of tracer in the application site -a potential confounding factor in nerve regeneration studies
title_full Persistence of tracer in the application site -a potential confounding factor in nerve regeneration studies
title_fullStr Persistence of tracer in the application site -a potential confounding factor in nerve regeneration studies
title_full_unstemmed Persistence of tracer in the application site -a potential confounding factor in nerve regeneration studies
title_sort Persistence of tracer in the application site -a potential confounding factor in nerve regeneration studies
dc.creator.none.fl_str_mv Puigdellívol Sánchez, Anna
Prats Galino, Alberto
Ruano Gil, Domingo
Molander, Carl
author Puigdellívol Sánchez, Anna
author_facet Puigdellívol Sánchez, Anna
Prats Galino, Alberto
Ruano Gil, Domingo
Molander, Carl
author_role author
author2 Prats Galino, Alberto
Ruano Gil, Domingo
Molander, Carl
author2_role author
author
author
dc.subject.none.fl_str_mv Nervis perifèrics
Regeneració del sistema nerviós
Experimentació animal
Peripheral nerves
Nervous system regeneration
Animal experimentation
topic Nervis perifèrics
Regeneració del sistema nerviós
Experimentació animal
Peripheral nerves
Nervous system regeneration
Animal experimentation
description Selective reinnervation of peripheral targets after nerve injury might be assessed by injecting a first tracer in a target before nerve injury to label the original neuronal population, and applying a second tracer after the regeneration period to label the regenerated population. However, altered uptake of tracer, fading, and cell death may interfere with the results. Furthermore, if the first tracer injected remains in the target tissue, available for 're-uptake' by misdirected regenerating axons, which originally innervated another region, then the identification of the original population would be confused. With the aim of studying this problem, the sciatic nerve of adult rats was sectioned and sutured. After 3 days, to allow the distal axon to degenerate avoiding immediate retrograde transport, one of the dyes: Fast Blue (FB), Fluoro-Gold (FG) or Diamidino Yellow (DY), was injected into the tibial branch of the sciatic nerve, or in the skin of one of the denervated digits. Rats survived 2-3 months. The results showed labelled dorsal root ganglion (DRG) cells and motoneurones, indicating that late re-uptake of a first tracer occurs. This phenomenon must be considered when the model of sequential labelling is used for studying the accuracy of peripheral reinnervation.
publishDate 2003
dc.date.none.fl_str_mv 2003
2014
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/56405
url https://hdl.handle.net/2445/56405
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: http://dx.doi.org/10.1016/S0165-0270(03)00128-6
Journal of Neuroscience Methods, 2003, vol. 127, num. 1, p. 105 -110
http://dx.doi.org/10.1016/S0165-0270(03)00128-6
dc.rights.none.fl_str_mv (c) Elsevier B.V., 2003
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Elsevier B.V., 2003
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 18 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Fonaments Clínics)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.198674

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